Efficient performance of locally processed serum in vero cell culture

Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA softwa...

Full description

Bibliographic Details
Main Authors:	Ahmad Nor, Yusilawati, Jaafar, Nuhu Jaafar, Mel, Maizirwan
Format:	Article
Language:	English
Published:	David Publishing 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3305/ http://irep.iium.edu.my/3305/ http://irep.iium.edu.my/3305/1/Paper_Yusilawati_JMSE.pdf

id	iium-3305
recordtype	eprints
spelling	iium-33052013-12-13T01:47:23Z http://irep.iium.edu.my/3305/ Efficient performance of locally processed serum in vero cell culture Ahmad Nor, Yusilawati Jaafar, Nuhu Jaafar Mel, Maizirwan TP248.13 Biotechnology Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500 mL whole blood from disease free cattle using 50 mL anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30 min at 56 °C. Vero cells were cultured in 25 cm2 Tflasks with an initial cell seeding of 4.2 × 105 cells/mL. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 × 105 cells/mL) compared to commercial bovine serum (8.5 × 105 cell/mL and 6.78 × 105 cell/mL) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture. David Publishing 2011-04-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3305/1/Paper_Yusilawati_JMSE.pdf Ahmad Nor, Yusilawati and Jaafar, Nuhu Jaafar and Mel, Maizirwan (2011) Efficient performance of locally processed serum in vero cell culture. Journal of Materials Science and Engineering, 5 (4). pp. 439-446. ISSN 1934-8959 http://www.davidpublishing.com/index.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ahmad Nor, Yusilawati Jaafar, Nuhu Jaafar Mel, Maizirwan Efficient performance of locally processed serum in vero cell culture
description	Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500 mL whole blood from disease free cattle using 50 mL anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30 min at 56 °C. Vero cells were cultured in 25 cm2 Tflasks with an initial cell seeding of 4.2 × 105 cells/mL. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 × 105 cells/mL) compared to commercial bovine serum (8.5 × 105 cell/mL and 6.78 × 105 cell/mL) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture.
format	Article
author	Ahmad Nor, Yusilawati Jaafar, Nuhu Jaafar Mel, Maizirwan
author_facet	Ahmad Nor, Yusilawati Jaafar, Nuhu Jaafar Mel, Maizirwan
author_sort	Ahmad Nor, Yusilawati
title	Efficient performance of locally processed serum in vero cell culture
title_short	Efficient performance of locally processed serum in vero cell culture
title_full	Efficient performance of locally processed serum in vero cell culture
title_fullStr	Efficient performance of locally processed serum in vero cell culture
title_full_unstemmed	Efficient performance of locally processed serum in vero cell culture
title_sort	efficient performance of locally processed serum in vero cell culture
publisher	David Publishing
publishDate	2011
url	http://irep.iium.edu.my/3305/ http://irep.iium.edu.my/3305/ http://irep.iium.edu.my/3305/1/Paper_Yusilawati_JMSE.pdf
first_indexed	2023-09-18T20:11:05Z
last_indexed	2023-09-18T20:11:05Z
_version_	1777407482263502848

Efficient performance of locally processed serum in vero cell culture

Similar Items