Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)

Palm oil mill effluent (POME) constitutes 60% of the wastes generated in typical palm oil mill and its environmental impact has been identified to be harmful to aquatic lives. This project examined the potential of degrading POME with microorganisms that are indigenous to POME. Thus, two microorgani...

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Main Authors:	Alade, Abass O., Jameel, Ahmad Tariq, Muyibi, Suleyman Aremu, Abdul Karim, Mohamed Ismail, Alam, Md. Zahangir
Format:	Conference or Workshop Item
Language:	English English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://irep.iium.edu.my/32987/ http://irep.iium.edu.my/32987/ http://irep.iium.edu.my/32987/1/Assessment_of_extracellular_activities_of_novel_microorganisms_for_biodegradation_of_POME_%28POSTER_-2012%29.pdf http://irep.iium.edu.my/32987/3/ECB_2012-POME-Abass.pdf

id	iium-32987
recordtype	eprints
spelling	iium-329872016-06-05T08:25:08Z http://irep.iium.edu.my/32987/ Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME) Alade, Abass O. Jameel, Ahmad Tariq Muyibi, Suleyman Aremu Abdul Karim, Mohamed Ismail Alam, Md. Zahangir TP Chemical technology Palm oil mill effluent (POME) constitutes 60% of the wastes generated in typical palm oil mill and its environmental impact has been identified to be harmful to aquatic lives. This project examined the potential of degrading POME with microorganisms that are indigenous to POME. Thus, two microorganisms were isolated from POME using serial dilution (10−1 to 10−10) procedure on solid plates containing Potato Dextrose Agar (PDA), Sabouround Dextrose Agar (SDA) and Malt Extract Agar (MEA), respectively. The two microorganisms (TRQ1 and TRQ2) that consistently appeared on the three media plates were quantified and subjected to extracellular enzymatic activities such as amylolytic, gelatinolytic, cellulolytic and lipolytic. The two isolates, TRQ1 and TRQ2, showed negative response to amylolytic test and this confirmed that they are not fungi; however they are Gelatinolytic and Cellulolytic. TRQ1 showed low lipolytic activity while TRQ2 did not show any. The diameter covered by TRQ1 and TRQ2 on gelatin media were 8.5 and 8.0cm respectively, while the spread on cellulose media were 3.5 and 3.25 cm, respectively, on the seventh day. Furthermore, TRQ1 covered a diameter of 1.65cm while TRQ2 retained its initial diameter. These results show that TRQ1 and TRQ2 contain gelatinolytic and cellulolytic enzymes and can be utilized for the degradation of cellulosic substrates present in POME, in particular. The cheap and wide availability of the material (POME) used as source for the production of these microorganisms indicates their economic importance for industrial applications and environmental sustainability, particularly in converting waste to wealth. Keywords: Biodegradation; Cellulolytic; Gelatinolytic; Lipolytic; Microorganisms; POME 2012-09-15 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/32987/1/Assessment_of_extracellular_activities_of_novel_microorganisms_for_biodegradation_of_POME_%28POSTER_-2012%29.pdf application/pdf en http://irep.iium.edu.my/32987/3/ECB_2012-POME-Abass.pdf Alade, Abass O. and Jameel, Ahmad Tariq and Muyibi, Suleyman Aremu and Abdul Karim, Mohamed Ismail and Alam, Md. Zahangir (2012) Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME). In: IBS 2012, 23-26 September 2012, Istanbul, Turkey. http://dx.doi.org/10.1016/j.nbt.2012.08.280
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Alade, Abass O. Jameel, Ahmad Tariq Muyibi, Suleyman Aremu Abdul Karim, Mohamed Ismail Alam, Md. Zahangir Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)
description	Palm oil mill effluent (POME) constitutes 60% of the wastes generated in typical palm oil mill and its environmental impact has been identified to be harmful to aquatic lives. This project examined the potential of degrading POME with microorganisms that are indigenous to POME. Thus, two microorganisms were isolated from POME using serial dilution (10−1 to 10−10) procedure on solid plates containing Potato Dextrose Agar (PDA), Sabouround Dextrose Agar (SDA) and Malt Extract Agar (MEA), respectively. The two microorganisms (TRQ1 and TRQ2) that consistently appeared on the three media plates were quantified and subjected to extracellular enzymatic activities such as amylolytic, gelatinolytic, cellulolytic and lipolytic. The two isolates, TRQ1 and TRQ2, showed negative response to amylolytic test and this confirmed that they are not fungi; however they are Gelatinolytic and Cellulolytic. TRQ1 showed low lipolytic activity while TRQ2 did not show any. The diameter covered by TRQ1 and TRQ2 on gelatin media were 8.5 and 8.0cm respectively, while the spread on cellulose media were 3.5 and 3.25 cm, respectively, on the seventh day. Furthermore, TRQ1 covered a diameter of 1.65cm while TRQ2 retained its initial diameter. These results show that TRQ1 and TRQ2 contain gelatinolytic and cellulolytic enzymes and can be utilized for the degradation of cellulosic substrates present in POME, in particular. The cheap and wide availability of the material (POME) used as source for the production of these microorganisms indicates their economic importance for industrial applications and environmental sustainability, particularly in converting waste to wealth. Keywords: Biodegradation; Cellulolytic; Gelatinolytic; Lipolytic; Microorganisms; POME
format	Conference or Workshop Item
author	Alade, Abass O. Jameel, Ahmad Tariq Muyibi, Suleyman Aremu Abdul Karim, Mohamed Ismail Alam, Md. Zahangir
author_facet	Alade, Abass O. Jameel, Ahmad Tariq Muyibi, Suleyman Aremu Abdul Karim, Mohamed Ismail Alam, Md. Zahangir
author_sort	Alade, Abass O.
title	Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)
title_short	Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)
title_full	Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)
title_fullStr	Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)
title_full_unstemmed	Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)
title_sort	assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (pome)
publishDate	2012
url	http://irep.iium.edu.my/32987/ http://irep.iium.edu.my/32987/ http://irep.iium.edu.my/32987/1/Assessment_of_extracellular_activities_of_novel_microorganisms_for_biodegradation_of_POME_%28POSTER_-2012%29.pdf http://irep.iium.edu.my/32987/3/ECB_2012-POME-Abass.pdf
first_indexed	2023-09-18T20:47:39Z
last_indexed	2023-09-18T20:47:39Z
_version_	1777409782718660608

Assessment of extracellular activities of novel microorganisms for biodegradation of palm oil mill effluent (POME)

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