Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant a...

Full description

Bibliographic Details
Main Authors:	Mel, Maizirwan, Arifin, Mohd Azmir, Sohif, Hajar Naemah, Syed Hassan, Sharifah
Format:	Article
Language:	English
Published:	Malaysian Medical Associations 2010
Subjects:	QR355 Virology
Online Access:	http://irep.iium.edu.my/3291/ http://irep.iium.edu.my/3291/ http://irep.iium.edu.my/3291/1/Paper_MJM_Azmir.pdf

id	iium-3291
recordtype	eprints
spelling	iium-32912011-09-22T04:15:32Z http://irep.iium.edu.my/3291/ Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hassan, Sharifah QR355 Virology The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied. Malaysian Medical Associations 2010-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3291/1/Paper_MJM_Azmir.pdf Mel, Maizirwan and Arifin, Mohd Azmir and Sohif, Hajar Naemah and Syed Hassan, Sharifah (2010) Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor. Medical Journal of Malaysia, 65. pp. 19-21. ISSN 0300-5283 http://www.e-mjm.org/2010/Sup_tissue_engineering_content.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR355 Virology
spellingShingle	QR355 Virology Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hassan, Sharifah Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
description	The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied.
format	Article
author	Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hassan, Sharifah
author_facet	Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hassan, Sharifah
author_sort	Mel, Maizirwan
title	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_short	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_full	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_fullStr	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_full_unstemmed	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_sort	optimization of process conditions for high cell density proliferation of df-1 cells in bioreactor
publisher	Malaysian Medical Associations
publishDate	2010
url	http://irep.iium.edu.my/3291/ http://irep.iium.edu.my/3291/ http://irep.iium.edu.my/3291/1/Paper_MJM_Azmir.pdf
first_indexed	2023-09-18T20:11:04Z
last_indexed	2023-09-18T20:11:04Z
_version_	1777407480962220032

Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

Similar Items