Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes

Owing to the current worsening situation of the increasing burden of diabetes around the world including Malaysia it is worthwhile to discover non-pharmacological prevention or treatment for it. In order to have a useful explanation of the efficacy of herbs or nutrients for diabetes; it is desirable...

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Main Authors:	Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher, Arief Ichwan, Solachuddin Jauhari, Azahari, Nuraniza
Format:	Article
Language:	English
Published:	Elsevier 2013
Subjects:	RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/32905/ http://irep.iium.edu.my/32905/ http://irep.iium.edu.my/32905/ http://irep.iium.edu.my/32905/1/Procedia_Behaviour_Sci.pdf

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recordtype	eprints
spelling	iium-329052019-09-03T02:21:17Z http://irep.iium.edu.my/32905/ Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Azahari, Nuraniza RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica Owing to the current worsening situation of the increasing burden of diabetes around the world including Malaysia it is worthwhile to discover non-pharmacological prevention or treatment for it. In order to have a useful explanation of the efficacy of herbs or nutrients for diabetes; it is desirable to know the effect on the balance between the adipogenesis, adipolysis and glucose uptake in the adipose tissues. Therefore, four herbs namely Orthosiphon stamineus (Cat whisker) (OS), Peronema canescens (Sungkai) (PC), Momordica charantia (Bitter gourd/bitter melon) (MC) and Pithecellobium jiringa (Jering) (PJ) were screened for their antidiabetic properties in in vitro model 3T3-L1 adipocytes. Water extracts of these herbs were prepared and evaluated for their effects on cell proliferation, adipogenesis, adipolysis and glucose uptake in 3T3-L1 preadipocytes cells. The aforementioned extracts promoted cell proliferation at a dose of 0.25mg/ml which showed more than 90% viability after 48 hours of treatment. The result of this study indicates that OS extracts significantly (P<0.001) increased adipogenesis whereas PC, MC and PJ extracts were not effective compared to control. The extracts from all four plants caused increased lipolysis compared to control. The Extract from OS and PJ significantly (P<0.05) stimulated glucose uptake in the cells whereas PC, MC were not effective. When the glucose consumption was compared to control it was significantly (P<0.001) increased for all extracts in the medium. The present study provides some important baseline data on the biochemical aspects of the effect induced by the herbs and suggestive of possessing antidiabetic properties which can be exploited for diabetes prevention and associated metabolic dysfunctions. Elsevier 2013-10-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32905/1/Procedia_Behaviour_Sci.pdf Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher and Arief Ichwan, Solachuddin Jauhari and Azahari, Nuraniza (2013) Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes. Procedia - Social and Behavioral Sciences, 91. pp. 357-375. ISSN 1877-0428 http://www.sciencedirect.com/science/article/pii/S1877042813025640 10.1016/j.sbspro.2013.08.433
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica
spellingShingle	RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Azahari, Nuraniza Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes
description	Owing to the current worsening situation of the increasing burden of diabetes around the world including Malaysia it is worthwhile to discover non-pharmacological prevention or treatment for it. In order to have a useful explanation of the efficacy of herbs or nutrients for diabetes; it is desirable to know the effect on the balance between the adipogenesis, adipolysis and glucose uptake in the adipose tissues. Therefore, four herbs namely Orthosiphon stamineus (Cat whisker) (OS), Peronema canescens (Sungkai) (PC), Momordica charantia (Bitter gourd/bitter melon) (MC) and Pithecellobium jiringa (Jering) (PJ) were screened for their antidiabetic properties in in vitro model 3T3-L1 adipocytes. Water extracts of these herbs were prepared and evaluated for their effects on cell proliferation, adipogenesis, adipolysis and glucose uptake in 3T3-L1 preadipocytes cells. The aforementioned extracts promoted cell proliferation at a dose of 0.25mg/ml which showed more than 90% viability after 48 hours of treatment. The result of this study indicates that OS extracts significantly (P<0.001) increased adipogenesis whereas PC, MC and PJ extracts were not effective compared to control. The extracts from all four plants caused increased lipolysis compared to control. The Extract from OS and PJ significantly (P<0.05) stimulated glucose uptake in the cells whereas PC, MC were not effective. When the glucose consumption was compared to control it was significantly (P<0.001) increased for all extracts in the medium. The present study provides some important baseline data on the biochemical aspects of the effect induced by the herbs and suggestive of possessing antidiabetic properties which can be exploited for diabetes prevention and associated metabolic dysfunctions.
format	Article
author	Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Azahari, Nuraniza
author_facet	Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Azahari, Nuraniza
author_sort	Khattak, Muhammad Muzaffar Ali Khan
title	Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes
title_short	Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes
title_full	Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes
title_fullStr	Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes
title_full_unstemmed	Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes
title_sort	selected herbal extracts improve diabetes associated factors in 3t3-l1 adipocytes
publisher	Elsevier
publishDate	2013
url	http://irep.iium.edu.my/32905/ http://irep.iium.edu.my/32905/ http://irep.iium.edu.my/32905/ http://irep.iium.edu.my/32905/1/Procedia_Behaviour_Sci.pdf
first_indexed	2023-09-18T20:47:32Z
last_indexed	2023-09-18T20:47:32Z
_version_	1777409774938226688

Selected herbal extracts improve diabetes associated factors in 3T3-L1 adipocytes

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