Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes

Moringa oleifera seed extracts has been found to possesses antibacterial properties. In this research, the antibacterial property of the defatted seed extract (after oil removal) was investigated against two gram-negative bacterial strains (Escherichia coli, Pseudosomonas aeruginosa) and two gram-...

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Main Authors:	Idris Oseni, Munirat, Muyibi, Suleyman Aremu, Abdulkarim, Mohd Ismail, Jamal, Parveen, Hamzah, Mohd. Salleh, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	Progress Publishing Company 2013
Subjects:	TD Environmental technology. Sanitary engineering
Online Access:	http://irep.iium.edu.my/32231/ http://irep.iium.edu.my/32231/ http://irep.iium.edu.my/32231/ http://irep.iium.edu.my/32231/1/Munirat_2013%2821A-16%29%5B1%5D_%28IJAR%29.pdf

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spelling	iium-322312013-10-08T01:49:56Z http://irep.iium.edu.my/32231/ Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes Idris Oseni, Munirat Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail Jamal, Parveen Hamzah, Mohd. Salleh Jami, Mohammed Saedi TD Environmental technology. Sanitary engineering Moringa oleifera seed extracts has been found to possesses antibacterial properties. In this research, the antibacterial property of the defatted seed extract (after oil removal) was investigated against two gram-negative bacterial strains (Escherichia coli, Pseudosomonas aeruginosa) and two gram-positive bacterial strains (Bacillus subtilis, Staphylococcus aureus). Water and 1 Molar salt solution was used to prepare the defatted seed extracts. Susceptibility tests were carried out using the agar well diffusion method. The effect of increase in concentration of the seed extract was investigated and the Minimum Inhibitory Concentration (MIC) as well as Minimum Bactericidal Concentration (MBC) was also carried out. The results obtained showed that the diameter inhibition zone ranges from 13mm to 30mm, increase in the seed extracts showed a corresponding increase in the inhibition of the bacterial strains. The results also revealed that salt extract f the seed showed higher increase in the antibacterial activity of the seed extract against the bacterial strains when compared with the water seed extract. MIC values of defatted water seed extract obtained were 0.39mg/ml, 0.39mg/ml, 0.78mg/ml, and 0.78mg/ml for Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus respectively and MIC obtained for the salt solution seed extract is 0.78mg/ml, 0.78mg/ml, 0.78mg/ml, 0.78mg/ml for Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus respectively. The mode of attack of the seed extracts show that it is strongly bactericidal against Escherichia coli, Pseudosomonas aeruginosa and bacteriostatic against Bacillus subtilis, and Staphylococcus aureus Key words: Moringa oleifera; antibacterial property; MIC; MBC Progress Publishing Company 2013-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32231/1/Munirat_2013%2821A-16%29%5B1%5D_%28IJAR%29.pdf Idris Oseni, Munirat and Muyibi, Suleyman Aremu and Abdulkarim, Mohd Ismail and Jamal, Parveen and Hamzah, Mohd. Salleh and Jami, Mohammed Saedi (2013) Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes. International Journal of Academic Research, 5 (1). pp. 101-106. ISSN 2075-4124 http://www.ijar.lit.az/en.php?go=january2013 DOI: dx.doi.org/10.7813/2075-4124.2013/5-1/A.16
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TD Environmental technology. Sanitary engineering
spellingShingle	TD Environmental technology. Sanitary engineering Idris Oseni, Munirat Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail Jamal, Parveen Hamzah, Mohd. Salleh Jami, Mohammed Saedi Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes
description	Moringa oleifera seed extracts has been found to possesses antibacterial properties. In this research, the antibacterial property of the defatted seed extract (after oil removal) was investigated against two gram-negative bacterial strains (Escherichia coli, Pseudosomonas aeruginosa) and two gram-positive bacterial strains (Bacillus subtilis, Staphylococcus aureus). Water and 1 Molar salt solution was used to prepare the defatted seed extracts. Susceptibility tests were carried out using the agar well diffusion method. The effect of increase in concentration of the seed extract was investigated and the Minimum Inhibitory Concentration (MIC) as well as Minimum Bactericidal Concentration (MBC) was also carried out. The results obtained showed that the diameter inhibition zone ranges from 13mm to 30mm, increase in the seed extracts showed a corresponding increase in the inhibition of the bacterial strains. The results also revealed that salt extract f the seed showed higher increase in the antibacterial activity of the seed extract against the bacterial strains when compared with the water seed extract. MIC values of defatted water seed extract obtained were 0.39mg/ml, 0.39mg/ml, 0.78mg/ml, and 0.78mg/ml for Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus respectively and MIC obtained for the salt solution seed extract is 0.78mg/ml, 0.78mg/ml, 0.78mg/ml, 0.78mg/ml for Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus respectively. The mode of attack of the seed extracts show that it is strongly bactericidal against Escherichia coli, Pseudosomonas aeruginosa and bacteriostatic against Bacillus subtilis, and Staphylococcus aureus Key words: Moringa oleifera; antibacterial property; MIC; MBC
format	Article
author	Idris Oseni, Munirat Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail Jamal, Parveen Hamzah, Mohd. Salleh Jami, Mohammed Saedi
author_facet	Idris Oseni, Munirat Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail Jamal, Parveen Hamzah, Mohd. Salleh Jami, Mohammed Saedi
author_sort	Idris Oseni, Munirat
title	Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes
title_short	Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes
title_full	Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes
title_fullStr	Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes
title_full_unstemmed	Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes
title_sort	investigating antibacterial activity of defatted moringa oleifera seed extract on selected gram-negative and gram-positive microbes
publisher	Progress Publishing Company
publishDate	2013
url	http://irep.iium.edu.my/32231/ http://irep.iium.edu.my/32231/ http://irep.iium.edu.my/32231/ http://irep.iium.edu.my/32231/1/Munirat_2013%2821A-16%29%5B1%5D_%28IJAR%29.pdf
first_indexed	2023-09-18T20:46:31Z
last_indexed	2023-09-18T20:46:31Z
_version_	1777409711791931392

Investigating antibacterial activity of defatted Moringa Oleifera seed extract on selected gram-negative and gram-positive microbes

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