In-vitro antibacterial activity of crude defatted Moringa Oleifera seed extract: kill time study
Earlier research conducted on moringa oleifera seed extract not only reveals its excellent coagulant property; it also being proven to possesses antibacterial activity against microorganism. An antibacterial agent 4(α-L- rhamnosyloxy) benzyl isothiocyanate, a plant synthesized derivatives of benz...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
INSI Publications
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/32221/ http://irep.iium.edu.my/32221/ http://irep.iium.edu.my/32221/1/Munirat-149-153_2%28AJBAS%29.pdf |
| Summary: | Earlier research conducted on moringa oleifera seed extract not only reveals its excellent
coagulant property; it also being proven to possesses antibacterial activity against microorganism. An
antibacterial agent 4(α-L- rhamnosyloxy) benzyl isothiocyanate, a plant synthesized derivatives of
benzyl isothiocyanates has been identified as the active antibacterial agent present in the seed. In this
study, the mode of antibacterial action was determined using the kill-time study on e.coil and
pseudosomonas aeruginosa bacterial strains. The minimum inhibitory concentration (MIC) assay was
done using macrodilution broth. MIC values were varied from 0.0125mg/ml to 0.1mg/ml. Kill-time
assay was carried out based on standard procedure. The growth of the microbes was monitored for
every 30 minutes by viability counting on agar plate. Log reduction of viable cells counts ranged from
0 to 3.6 log10 for e.coli and 1log10 to 4log10 for p.aeruginosa. The results of the kill-time study
revealed that the extract was bactericidal against e.coli and p.aeruginosa within 30 minutes of contact
resulting in about 90% elimination of the strains. Most of the microbes were killed at high MIC value
of 0.1 mg/ml, which might suggest that the seed extract exhibits concentration-dependent killing that
revealed that it can be used as a natural disinfectant for water treatment.
Key words: benzyl isothiocyanates; Moringa oleifera; kill-time; e.coli; p. aeruginosa |
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