Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp

The potential of cassava starch and its leaves for lactic acid production was investigated using fungal Rhizopus sp. in fermentation process. The minerals content such as Zinc, Ferum and Magnesium and cyanide content in cassava leaves were investigated. The results showed that cassava leaves contain...

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Main Authors:	Yusuf, Nurlisa, Azmi, Azlin Suhaida, Jimat, Dzun Noraini
Format:	Conference or Workshop Item
Language:	English English English English English
Published:	2012
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/32124/ http://irep.iium.edu.my/32124/2/IBS_2012-azlin-presentation_slides.pdf http://irep.iium.edu.my/32124/1/IBS_program.pdf http://irep.iium.edu.my/32124/3/Abstract_IBS_2012.pdf http://irep.iium.edu.my/32124/9/IBS_2012_acceptance_letter.pdf http://irep.iium.edu.my/32124/10/IBS_2012_cover_page_program_book.pdf

id	iium-32124
recordtype	eprints
spelling	iium-321242013-09-26T06:18:13Z http://irep.iium.edu.my/32124/ Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp Yusuf, Nurlisa Azmi, Azlin Suhaida Jimat, Dzun Noraini TP248.13 Biotechnology The potential of cassava starch and its leaves for lactic acid production was investigated using fungal Rhizopus sp. in fermentation process. The minerals content such as Zinc, Ferum and Magnesium and cyanide content in cassava leaves were investigated. The results showed that cassava leaves contain 0.12 mg/L Zn, 0.3 mg/L Fe and 2.64 mg/L Mg and no cyanogenic glycoside in leaf after dried in oven at 80ºC for 24 hours. For the optimization of hydrolysis process, three factors such as acid and starch concentrations and temperature were investigated. The maximum result of a hydrolysis process was 26.58 g/L glucose concentration obtained from Run 2 which was utilizing 25 g/L of starch and 0.20 M of HNO3 at 160ºC. Next, the fermentation process was conducted in shake flask to determine the best condition for lactic acid production. There were three different conditions affecting the concentration of lactic acid production such as inoculum dilution, temperature and agitation. The maximum lactic acid concentration of 24.61 g/L were determined from Run 2 at temperature of 30ºC using 25 ml inoculum dilution and agitated at 200 rpm. Besides, the maximum ethanol concentration was 13.49 g/L at Run 4 which was conducted at the same temperature as Run 2 but at different agitation speed (250 rpm) at pH 7. Design Expert software v6.0.8 was used to design the experiment for reducing the run of the experiment during hydrolysis and fermentation analysis using Taguchi's method by selecting the significant parameters. All results have been analyzed by High Performance Liquid Chromatography (HPLC) using the refractive index detector. 2012-09-16 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/32124/2/IBS_2012-azlin-presentation_slides.pdf application/pdf en http://irep.iium.edu.my/32124/1/IBS_program.pdf application/pdf en http://irep.iium.edu.my/32124/3/Abstract_IBS_2012.pdf application/pdf en http://irep.iium.edu.my/32124/9/IBS_2012_acceptance_letter.pdf application/pdf en http://irep.iium.edu.my/32124/10/IBS_2012_cover_page_program_book.pdf Yusuf, Nurlisa and Azmi, Azlin Suhaida and Jimat, Dzun Noraini (2012) Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp. In: 15th International Biotechnology Symposyiom & Exhibition (IBS 2012), 16-21 Sept 2012, Daegu, Republic of Korea. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Yusuf, Nurlisa Azmi, Azlin Suhaida Jimat, Dzun Noraini Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp
description	The potential of cassava starch and its leaves for lactic acid production was investigated using fungal Rhizopus sp. in fermentation process. The minerals content such as Zinc, Ferum and Magnesium and cyanide content in cassava leaves were investigated. The results showed that cassava leaves contain 0.12 mg/L Zn, 0.3 mg/L Fe and 2.64 mg/L Mg and no cyanogenic glycoside in leaf after dried in oven at 80ºC for 24 hours. For the optimization of hydrolysis process, three factors such as acid and starch concentrations and temperature were investigated. The maximum result of a hydrolysis process was 26.58 g/L glucose concentration obtained from Run 2 which was utilizing 25 g/L of starch and 0.20 M of HNO3 at 160ºC. Next, the fermentation process was conducted in shake flask to determine the best condition for lactic acid production. There were three different conditions affecting the concentration of lactic acid production such as inoculum dilution, temperature and agitation. The maximum lactic acid concentration of 24.61 g/L were determined from Run 2 at temperature of 30ºC using 25 ml inoculum dilution and agitated at 200 rpm. Besides, the maximum ethanol concentration was 13.49 g/L at Run 4 which was conducted at the same temperature as Run 2 but at different agitation speed (250 rpm) at pH 7. Design Expert software v6.0.8 was used to design the experiment for reducing the run of the experiment during hydrolysis and fermentation analysis using Taguchi's method by selecting the significant parameters. All results have been analyzed by High Performance Liquid Chromatography (HPLC) using the refractive index detector.
format	Conference or Workshop Item
author	Yusuf, Nurlisa Azmi, Azlin Suhaida Jimat, Dzun Noraini
author_facet	Yusuf, Nurlisa Azmi, Azlin Suhaida Jimat, Dzun Noraini
author_sort	Yusuf, Nurlisa
title	Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp
title_short	Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp
title_full	Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp
title_fullStr	Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp
title_full_unstemmed	Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp
title_sort	production of lactic acid from fermentation of pretreated cassava starch and leaves using rhizopus sp
publishDate	2012
url	http://irep.iium.edu.my/32124/ http://irep.iium.edu.my/32124/2/IBS_2012-azlin-presentation_slides.pdf http://irep.iium.edu.my/32124/1/IBS_program.pdf http://irep.iium.edu.my/32124/3/Abstract_IBS_2012.pdf http://irep.iium.edu.my/32124/9/IBS_2012_acceptance_letter.pdf http://irep.iium.edu.my/32124/10/IBS_2012_cover_page_program_book.pdf
first_indexed	2023-09-18T20:46:21Z
last_indexed	2023-09-18T20:46:21Z
_version_	1777409701100650496

Production of lactic acid from fermentation of pretreated cassava starch and leaves using Rhizopus sp

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