Kinetic studies on recombinant stem bromelain

Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carb...

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Main Authors:	Bala, Muntari, Mel, Maizirwan, Jami, Mohammed Saedi, Amid, Azura, Salleh, Hamzah Mohd.
Format:	Article
Language:	English
Published:	SciRes. 2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/1/Kinetic_study_of_recombinant_stem_bromelain.pdf

id	iium-32114
recordtype	eprints
spelling	iium-321142013-10-02T04:41:27Z http://irep.iium.edu.my/32114/ Kinetic studies on recombinant stem bromelain Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd. TP248.13 Biotechnology Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carbobenzoxy-L-arginyl-L-ar-ginine-p-nitroanilide (ZAANA), N-α-carbobenzo-xy-L-phenylalanyl-L-valyl-L-arginine-p-nitroanili-de (ZPVANA) and L-pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA). Hydrolytic activities of recBM at various pH and temperature conditions were compared to that of commercial bromelain (cBM). Both enzymes demonstrated high activities at 45o C and pH 5 - 8 for recBM and pH 6 - 8 for cBM. recBM showed marginally lower Kmand slightly higher kcat/Kmfor ZAANA, ZANPE and ZPVANA in comparison to cBM.trans Epoxysuccinyl-L-leucylamido {4- guanidino}butane (E-64) severely affected recBM and cBM hydrolysis of the synthetic substrates by competitive inhibition with Kivalues of 3.6 - 5.1 μM and 5.5 - 6.9 μM for recBM and cBM, respectively. The evaluated properties of recBM including temperature and pH optima, substrate specificity and sensitivity to inhibitors or activators, satisfy the requisites required for food industries. SciRes. 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32114/1/Kinetic_study_of_recombinant_stem_bromelain.pdf Bala, Muntari and Mel, Maizirwan and Jami, Mohammed Saedi and Amid, Azura and Salleh, Hamzah Mohd. (2013) Kinetic studies on recombinant stem bromelain. Advances in Enzyme Research, 1 (3). pp. 52-60. ISSN 2328-4846 http://www.scirp.org/journal/aer/ DOI: 10.4236/aer.2013.13006
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd. Kinetic studies on recombinant stem bromelain
description	Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carbobenzoxy-L-arginyl-L-ar-ginine-p-nitroanilide (ZAANA), N-α-carbobenzo-xy-L-phenylalanyl-L-valyl-L-arginine-p-nitroanili-de (ZPVANA) and L-pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA). Hydrolytic activities of recBM at various pH and temperature conditions were compared to that of commercial bromelain (cBM). Both enzymes demonstrated high activities at 45o C and pH 5 - 8 for recBM and pH 6 - 8 for cBM. recBM showed marginally lower Kmand slightly higher kcat/Kmfor ZAANA, ZANPE and ZPVANA in comparison to cBM.trans Epoxysuccinyl-L-leucylamido {4- guanidino}butane (E-64) severely affected recBM and cBM hydrolysis of the synthetic substrates by competitive inhibition with Kivalues of 3.6 - 5.1 μM and 5.5 - 6.9 μM for recBM and cBM, respectively. The evaluated properties of recBM including temperature and pH optima, substrate specificity and sensitivity to inhibitors or activators, satisfy the requisites required for food industries.
format	Article
author	Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd.
author_facet	Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd.
author_sort	Bala, Muntari
title	Kinetic studies on recombinant stem bromelain
title_short	Kinetic studies on recombinant stem bromelain
title_full	Kinetic studies on recombinant stem bromelain
title_fullStr	Kinetic studies on recombinant stem bromelain
title_full_unstemmed	Kinetic studies on recombinant stem bromelain
title_sort	kinetic studies on recombinant stem bromelain
publisher	SciRes.
publishDate	2013
url	http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/1/Kinetic_study_of_recombinant_stem_bromelain.pdf
first_indexed	2023-09-18T20:46:20Z
last_indexed	2023-09-18T20:46:20Z
_version_	1777409700075143168

Kinetic studies on recombinant stem bromelain

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