Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration

In dairy product sector, butter is one of the potential sources of fat soluble vitamins, namely vitamin A, D, E, K; consequently, butter is taken into account as high valuable price from other dairy products. This fact has attracted unscrupulous market players to blind butter with other animal fats...

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Main Authors:	Ahmad Fadzlillah, Nurrulhidayah, Rohman, Abdul, Ismail, Amin, Mustafa, Shuhaimi, Khatib, Alfi
Format:	Article
Language:	English
Published:	Japan Oil Chemists' Society 2013
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/32030/ http://irep.iium.edu.my/32030/ http://irep.iium.edu.my/32030/1/Nurrulhidayah_et_al.%2C_JOC_2013.pdf

id	iium-32030
recordtype	eprints
spelling	iium-320302020-02-18T02:51:14Z http://irep.iium.edu.my/32030/ Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration Ahmad Fadzlillah, Nurrulhidayah Rohman, Abdul Ismail, Amin Mustafa, Shuhaimi Khatib, Alfi QD Chemistry In dairy product sector, butter is one of the potential sources of fat soluble vitamins, namely vitamin A, D, E, K; consequently, butter is taken into account as high valuable price from other dairy products. This fact has attracted unscrupulous market players to blind butter with other animal fats to gain economic profit. Animal fats like mutton fat (MF) are potential to be mixed with butter due to the similarity in terms of fatty acid composition. This study focused on the application of FTIR-ATR spectroscopy in conjunction with chemometrics for classification and quantification of MF as adulterant in butter. The FTIR spectral region of 3910-710 cm–1 was used for classification between butter and butter blended with MF at various concentrations with the aid of discriminant analysis (DA). DA is able to classify butter and adulterated butter without any mistakenly grouped. For quantitative analysis, partial least square (PLS) regression was used to develop a calibration model at the frequency regions of 3910-710 cm–1. The equation obtained for the relationship between actual value of MF and FTIR predicted values of MF in PLS calibration model was y = 0.998x + 1.033, with the values of coefficient of determination (R2) and root mean square error of calibration are 0.998 and 0.046% (v/v), respectively. The PLS calibration model was subsequently used for the prediction of independent samples containing butter in the binary mixtures with MF. Using 9 principal components, root mean square error of prediction (RMSEP) is 1.68% (v/v). The results showed that FTIR spectroscopy can be used for the classification and quantification of MF in butter formulation for verification purposes. Japan Oil Chemists' Society 2013-08-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32030/1/Nurrulhidayah_et_al.%2C_JOC_2013.pdf Ahmad Fadzlillah, Nurrulhidayah and Rohman, Abdul and Ismail, Amin and Mustafa, Shuhaimi and Khatib, Alfi (2013) Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration. Journal of Oleo Science, 62 (8). pp. 1-8. ISSN 1347-3352 (O), 1345-8957 (P) https://www.jstage.jst.go.jp/article/jos/62/8/62_555/_article
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmad Fadzlillah, Nurrulhidayah Rohman, Abdul Ismail, Amin Mustafa, Shuhaimi Khatib, Alfi Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
description	In dairy product sector, butter is one of the potential sources of fat soluble vitamins, namely vitamin A, D, E, K; consequently, butter is taken into account as high valuable price from other dairy products. This fact has attracted unscrupulous market players to blind butter with other animal fats to gain economic profit. Animal fats like mutton fat (MF) are potential to be mixed with butter due to the similarity in terms of fatty acid composition. This study focused on the application of FTIR-ATR spectroscopy in conjunction with chemometrics for classification and quantification of MF as adulterant in butter. The FTIR spectral region of 3910-710 cm–1 was used for classification between butter and butter blended with MF at various concentrations with the aid of discriminant analysis (DA). DA is able to classify butter and adulterated butter without any mistakenly grouped. For quantitative analysis, partial least square (PLS) regression was used to develop a calibration model at the frequency regions of 3910-710 cm–1. The equation obtained for the relationship between actual value of MF and FTIR predicted values of MF in PLS calibration model was y = 0.998x + 1.033, with the values of coefficient of determination (R2) and root mean square error of calibration are 0.998 and 0.046% (v/v), respectively. The PLS calibration model was subsequently used for the prediction of independent samples containing butter in the binary mixtures with MF. Using 9 principal components, root mean square error of prediction (RMSEP) is 1.68% (v/v). The results showed that FTIR spectroscopy can be used for the classification and quantification of MF in butter formulation for verification purposes.
format	Article
author	Ahmad Fadzlillah, Nurrulhidayah Rohman, Abdul Ismail, Amin Mustafa, Shuhaimi Khatib, Alfi
author_facet	Ahmad Fadzlillah, Nurrulhidayah Rohman, Abdul Ismail, Amin Mustafa, Shuhaimi Khatib, Alfi
author_sort	Ahmad Fadzlillah, Nurrulhidayah
title	Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
title_short	Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
title_full	Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
title_fullStr	Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
title_full_unstemmed	Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
title_sort	application of ftir-atr spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration
publisher	Japan Oil Chemists' Society
publishDate	2013
url	http://irep.iium.edu.my/32030/ http://irep.iium.edu.my/32030/ http://irep.iium.edu.my/32030/1/Nurrulhidayah_et_al.%2C_JOC_2013.pdf
first_indexed	2023-09-18T20:46:13Z
last_indexed	2023-09-18T20:46:13Z
_version_	1777409692039905280

Application of FTIR-ATR spectroscopy coupled with multivariate analysis for rapid estimation of butter adulteration

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