LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study

A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using gli...

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Main Authors:	Sengupta, Pinaki, Bhaumik, Uttam, Ghosh, Animesh, Sarkar, Amlan Kanti, Chatterjee, Bappaditya, Bose, Anirbandeep, Pal, Tapan Kumar
Format:	Article
Language:	English
Published:	Vieweg Verlag 2009
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/31908/ http://irep.iium.edu.my/31908/ http://irep.iium.edu.my/31908/ http://irep.iium.edu.my/31908/1/Paper6.pdf

id	iium-31908
recordtype	eprints
spelling	iium-319082013-09-20T03:10:46Z http://irep.iium.edu.my/31908/ LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar R Medicine (General) A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using glipizide as an internal standard. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. Inter-batch and intra-batch coefficient of variation across four validation runs for the quality control samples was less than 7%. The accuracy determined at quality control levels was within 92.81–105.13%. The method was applied to a bioequivalence study. Vieweg Verlag 2009-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31908/1/Paper6.pdf Sengupta, Pinaki and Bhaumik, Uttam and Ghosh, Animesh and Sarkar, Amlan Kanti and Chatterjee, Bappaditya and Bose, Anirbandeep and Pal, Tapan Kumar (2009) LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study. Chromatographia, 69 (11/12). pp. 1243-1250. ISSN 1612-1112 (O), 0009-5893 (P) http://link.springer.com/article/10.1365%2Fs10337-009-1056-5 10.1365/s10337-009-1056-5
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
description	A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the Food and Drug Administration guidelines for the simultaneous quantitation of antidiabetic drugs metformin, glimiperide and pioglitazone in human plasma using glipizide as an internal standard. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. Inter-batch and intra-batch coefficient of variation across four validation runs for the quality control samples was less than 7%. The accuracy determined at quality control levels was within 92.81–105.13%. The method was applied to a bioequivalence study.
format	Article
author	Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar
author_facet	Sengupta, Pinaki Bhaumik, Uttam Ghosh, Animesh Sarkar, Amlan Kanti Chatterjee, Bappaditya Bose, Anirbandeep Pal, Tapan Kumar
author_sort	Sengupta, Pinaki
title	LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
title_short	LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
title_full	LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
title_fullStr	LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
title_full_unstemmed	LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
title_sort	lc-ms-ms development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study
publisher	Vieweg Verlag
publishDate	2009
url	http://irep.iium.edu.my/31908/ http://irep.iium.edu.my/31908/ http://irep.iium.edu.my/31908/ http://irep.iium.edu.my/31908/1/Paper6.pdf
first_indexed	2023-09-18T20:46:03Z
last_indexed	2023-09-18T20:46:03Z
_version_	1777409682101501952

LC-MS-MS development and validation for simultaneous quantitation of metformin, glimepiride and pioglitazone in human plasma and its application to a bioequivalence study

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