Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree

Lignin is an important constitute of the wood and thus the organism like fungi and insects growing on wood produce some extracellular lignolytic enzymes. In fungi these enzymes include different oxidases and peroxidases. Lignin peroxidases, manganese peroxidases, versatile peroxidases and laccases h...

Full description

Bibliographic Details
Main Authors:	Moin, Syed Faraz, Alfarra, Helmi Yousif, Omar, Muhammad Nor
Format:	Article
Language:	English
Published:	Oriental Scientific Publishing Company 2012
Subjects:	QK Botany
Online Access:	http://irep.iium.edu.my/31598/ http://irep.iium.edu.my/31598/ http://irep.iium.edu.my/31598/1/BBRA_2012.pdf

id	iium-31598
recordtype	eprints
spelling	iium-315982015-12-11T13:29:14Z http://irep.iium.edu.my/31598/ Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree Moin, Syed Faraz Alfarra, Helmi Yousif Omar, Muhammad Nor QK Botany Lignin is an important constitute of the wood and thus the organism like fungi and insects growing on wood produce some extracellular lignolytic enzymes. In fungi these enzymes include different oxidases and peroxidases. Lignin peroxidases, manganese peroxidases, versatile peroxidases and laccases have been characterised from fungi and their roles in wood decay has been established. Here we report ABTS-oxidases from an unidentified fungus isolated from tree bark sample from Kuantan, Malaysia. After the liquid media cultivation, the crude extracellular protein content was extracted and analysed by polyacrylamide gel electrophoresis. SDS-PAGE, native PAGE and zymography were carried out. Azo dyes, brilliant green, trypan blue, direct red 80 and eriochrome black T were used for decolourisation activity of the isolated fungus on solid medium. The results suggest that the extracellular proteins contain laccase enzyme activity. Out of the four azo dyes that were used for dye decolourisation assay, at least three were decolourised to different extents by the fungus. Oriental Scientific Publishing Company 2012-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31598/1/BBRA_2012.pdf Moin, Syed Faraz and Alfarra, Helmi Yousif and Omar, Muhammad Nor (2012) Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree. Biosciences Biotechnology Research Asia, 9 (2). pp. 641-646. ISSN 0973-1245 http://www.biotech-asia.org/abstract/Syed-Faraz-Moin-Helmi-Yousuf-Alfarra-and-Muhammad-Nor-Omar/Azo-dyes-decolourisation-by-ABTS-oxidases-Laccases-from-a-fungus-from-tropical-tree/1044.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QK Botany
spellingShingle	QK Botany Moin, Syed Faraz Alfarra, Helmi Yousif Omar, Muhammad Nor Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree
description	Lignin is an important constitute of the wood and thus the organism like fungi and insects growing on wood produce some extracellular lignolytic enzymes. In fungi these enzymes include different oxidases and peroxidases. Lignin peroxidases, manganese peroxidases, versatile peroxidases and laccases have been characterised from fungi and their roles in wood decay has been established. Here we report ABTS-oxidases from an unidentified fungus isolated from tree bark sample from Kuantan, Malaysia. After the liquid media cultivation, the crude extracellular protein content was extracted and analysed by polyacrylamide gel electrophoresis. SDS-PAGE, native PAGE and zymography were carried out. Azo dyes, brilliant green, trypan blue, direct red 80 and eriochrome black T were used for decolourisation activity of the isolated fungus on solid medium. The results suggest that the extracellular proteins contain laccase enzyme activity. Out of the four azo dyes that were used for dye decolourisation assay, at least three were decolourised to different extents by the fungus.
format	Article
author	Moin, Syed Faraz Alfarra, Helmi Yousif Omar, Muhammad Nor
author_facet	Moin, Syed Faraz Alfarra, Helmi Yousif Omar, Muhammad Nor
author_sort	Moin, Syed Faraz
title	Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree
title_short	Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree
title_full	Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree
title_fullStr	Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree
title_full_unstemmed	Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree
title_sort	azo dyes decolourisation by abts-oxidases (laccases) from a fungus from tropical tree
publisher	Oriental Scientific Publishing Company
publishDate	2012
url	http://irep.iium.edu.my/31598/ http://irep.iium.edu.my/31598/ http://irep.iium.edu.my/31598/1/BBRA_2012.pdf
first_indexed	2023-09-18T20:45:46Z
last_indexed	2023-09-18T20:45:46Z
_version_	1777409663737790464

Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree

Similar Items