Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)

The antioxidant activity and the total phenolic content, as well as the influence of petroleum ether, chloroform and methanol extracts from the leaves of Gynotroches axillaris, on micro- organisms were studied. The total phenolic contents were evaluated by using Folin-Ciocalteu reagent and the obtai...

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Main Authors:	Abed, Salam Ahmed, Mohd Sirat, Hasnah, Bakhtiar, M. Taher
Format:	Article
Language:	English
Published:	University of Mainz 2013
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/31208/ http://irep.iium.edu.my/31208/ http://irep.iium.edu.my/31208/1/EXCLI_Sirat_13052013_proof.pdf

id	iium-31208
recordtype	eprints
spelling	iium-312082014-12-08T09:47:42Z http://irep.iium.edu.my/31208/ Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae) Abed, Salam Ahmed Mohd Sirat, Hasnah Bakhtiar, M. Taher RS Pharmacy and materia medica The antioxidant activity and the total phenolic content, as well as the influence of petroleum ether, chloroform and methanol extracts from the leaves of Gynotroches axillaris, on micro- organisms were studied. The total phenolic contents were evaluated by using Folin-Ciocalteu reagent and the obtained values ranged from 70.0 to 620 mg GAE/g. The efficiency of antiox- idation, which was identified through the scavenging of free radical DPPH, exhibited that the highest IC50 was in the methanolic extract (44.7 μg/mL) as compared to the standard ascorbic acid (25.83 μg/mL) and to standard BHT (17.2 μg/mL). In vitro antimicrobial activity of ex- tracts was tested against Gram-negative bacteria, Gram-positive bacteria and fungi. Methanol extract showed activity in the range (225-900 μg/mL) with both types, while petroleum ether and chloroform extracts were only active with Bacillus subtilis. The three extracts strongly inhibited all fungi with activity 225-450 μg/mL. The toxicity test against brine shrimps indi- cated that all extracts were non-toxic with LC50 value more than 1000 μg/mL. The finding of this study supports the safety of these extracts to be used in medical treatments. University of Mainz 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31208/1/EXCLI_Sirat_13052013_proof.pdf Abed, Salam Ahmed and Mohd Sirat, Hasnah and Bakhtiar, M. Taher (2013) Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae). Excli Journal, 12. pp. 404-4012. ISSN 1611-2156 http://www.excli.de
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Abed, Salam Ahmed Mohd Sirat, Hasnah Bakhtiar, M. Taher Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)
description	The antioxidant activity and the total phenolic content, as well as the influence of petroleum ether, chloroform and methanol extracts from the leaves of Gynotroches axillaris, on micro- organisms were studied. The total phenolic contents were evaluated by using Folin-Ciocalteu reagent and the obtained values ranged from 70.0 to 620 mg GAE/g. The efficiency of antiox- idation, which was identified through the scavenging of free radical DPPH, exhibited that the highest IC50 was in the methanolic extract (44.7 μg/mL) as compared to the standard ascorbic acid (25.83 μg/mL) and to standard BHT (17.2 μg/mL). In vitro antimicrobial activity of ex- tracts was tested against Gram-negative bacteria, Gram-positive bacteria and fungi. Methanol extract showed activity in the range (225-900 μg/mL) with both types, while petroleum ether and chloroform extracts were only active with Bacillus subtilis. The three extracts strongly inhibited all fungi with activity 225-450 μg/mL. The toxicity test against brine shrimps indi- cated that all extracts were non-toxic with LC50 value more than 1000 μg/mL. The finding of this study supports the safety of these extracts to be used in medical treatments.
format	Article
author	Abed, Salam Ahmed Mohd Sirat, Hasnah Bakhtiar, M. Taher
author_facet	Abed, Salam Ahmed Mohd Sirat, Hasnah Bakhtiar, M. Taher
author_sort	Abed, Salam Ahmed
title	Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)
title_short	Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)
title_full	Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)
title_fullStr	Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)
title_full_unstemmed	Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)
title_sort	total phenolic, antioxidant, antimicrobial, activities and toxicity study of gynotroches axillaris blume (rhizophoraceae)
publisher	University of Mainz
publishDate	2013
url	http://irep.iium.edu.my/31208/ http://irep.iium.edu.my/31208/ http://irep.iium.edu.my/31208/1/EXCLI_Sirat_13052013_proof.pdf
first_indexed	2023-09-18T20:45:27Z
last_indexed	2023-09-18T20:45:27Z
_version_	1777409644133613568

Total phenolic, antioxidant, antimicrobial, activities and toxicity study of Gynotroches Axillaris Blume (Rhizophoraceae)

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