Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice

Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migra...

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Main Authors:	Abdalrahim , FA Aisha, Zhari I, Ismail, Khalid , M Abu-Saleh, Siddiqui, Mohammad Jamshed Ahmad, Ghafar, Gheniya, Shah, Amin Malik
Format:	Article
Language:	English
Published:	BioMed Central 2013
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf

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recordtype	eprints
spelling	iium-307912013-08-03T07:08:05Z http://irep.iium.edu.my/30791/ Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik QD Chemistry Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice. Results Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml). S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death. Conclusion Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid. BioMed Central 2013-07-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf Abdalrahim , FA Aisha and Zhari I, Ismail and Khalid , M Abu-Saleh and Siddiqui, Mohammad Jamshed Ahmad and Ghafar, Gheniya and Shah, Amin Malik (2013) Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice. BMC Complementary and Alternative Medicine (13). p. 168. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/13/168
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
description	Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice. Results Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml). S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death. Conclusion Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid.
format	Article
author	Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik
author_facet	Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik
author_sort	Abdalrahim , FA Aisha
title	Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_short	Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_full	Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_fullStr	Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_full_unstemmed	Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_sort	syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
publisher	BioMed Central
publishDate	2013
url	http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf
first_indexed	2023-09-18T20:45:01Z
last_indexed	2023-09-18T20:45:01Z
_version_	1777409617354031104

Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice

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