Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma

Object. Several genes have been shown to carry mutations in human malignant gliomas, including the phosphatase and tensin homolog (PTEN) deleted on chromosome 10 and p16 tumor suppressor genes. Alterations of this gene located on chromosome 10 q23 and 9p21, respectively, may contribute to gliomage...

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Main Authors:	Abdullah, Jafri Malin, Zainuddin, Norafiza, Sulong, Sarina, Isa, Mohd. Nizam, Jaafar, Hasnan
Format:	Article
Language:	English
Published:	Journal of Neurosurgery Publishing Group 2003
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/30769/ http://irep.iium.edu.my/30769/ http://irep.iium.edu.my/30769/1/Ja_Neurosurg_Focus.pdf

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recordtype	eprints
spelling	iium-307692013-07-19T01:06:44Z http://irep.iium.edu.my/30769/ Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma Abdullah, Jafri Malin Zainuddin, Norafiza Sulong, Sarina Isa, Mohd. Nizam Jaafar, Hasnan RC0254 Neoplasms. Tumors. Oncology (including Cancer) Object. Several genes have been shown to carry mutations in human malignant gliomas, including the phosphatase and tensin homolog (PTEN) deleted on chromosome 10 and p16 tumor suppressor genes. Alterations of this gene located on chromosome 10 q23 and 9p21, respectively, may contribute to gliomagenesis. In this study, the authors analyzed 20 cases of malignant gliomas obtained in patients living on the east coast of Malaysia to investigate the possibilities of involvement of the PTEN and p16 genes. Methods. Samples of DNA were amplified by polymerase chain reaction (PCR), analyzed by single-stranded conformation polymorphism (SSCP), and subsequently by sequencing. Two cases of glioblastoma multiforme, three cases of anaplastic astrocytoma, one case of anaplastic pleomorphic xanthoastrocytoma, and one case of anaplastic ependymoma showed SSCP band shifts in PTEN mutational analyses. The DNA sequencing analyses of these samples revealed missense and nonsense mutations, with cluster of mutations in the region 5� to the core phosphatase motif of exon 5 and the 5�-end of exon 6. No abnormal migration shifts were detected in the glioma samples analyzed for point mutations of the p16 gene. Homozygous deletions of p16 were also not detected in all samples. Conclusions. These findings indicate that mutations of the PTEN genes were likely to contribute to the tumorigenesis and morphological transformations of gliomas. In addition, the alterations of the p16 gene might not play a major role in tumorigenesis of malignant gliomas in Malaysian patients. Journal of Neurosurgery Publishing Group 2003-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30769/1/Ja_Neurosurg_Focus.pdf Abdullah, Jafri Malin and Zainuddin, Norafiza and Sulong, Sarina and Isa, Mohd. Nizam and Jaafar, Hasnan (2003) Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma. Neurosurgical Focus, 14 (4). pp. 1-7. ISSN 1092-0684 http://thejns.org/page/about
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Abdullah, Jafri Malin Zainuddin, Norafiza Sulong, Sarina Isa, Mohd. Nizam Jaafar, Hasnan Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
description	Object. Several genes have been shown to carry mutations in human malignant gliomas, including the phosphatase and tensin homolog (PTEN) deleted on chromosome 10 and p16 tumor suppressor genes. Alterations of this gene located on chromosome 10 q23 and 9p21, respectively, may contribute to gliomagenesis. In this study, the authors analyzed 20 cases of malignant gliomas obtained in patients living on the east coast of Malaysia to investigate the possibilities of involvement of the PTEN and p16 genes. Methods. Samples of DNA were amplified by polymerase chain reaction (PCR), analyzed by single-stranded conformation polymorphism (SSCP), and subsequently by sequencing. Two cases of glioblastoma multiforme, three cases of anaplastic astrocytoma, one case of anaplastic pleomorphic xanthoastrocytoma, and one case of anaplastic ependymoma showed SSCP band shifts in PTEN mutational analyses. The DNA sequencing analyses of these samples revealed missense and nonsense mutations, with cluster of mutations in the region 5� to the core phosphatase motif of exon 5 and the 5�-end of exon 6. No abnormal migration shifts were detected in the glioma samples analyzed for point mutations of the p16 gene. Homozygous deletions of p16 were also not detected in all samples. Conclusions. These findings indicate that mutations of the PTEN genes were likely to contribute to the tumorigenesis and morphological transformations of gliomas. In addition, the alterations of the p16 gene might not play a major role in tumorigenesis of malignant gliomas in Malaysian patients.
format	Article
author	Abdullah, Jafri Malin Zainuddin, Norafiza Sulong, Sarina Isa, Mohd. Nizam Jaafar, Hasnan
author_facet	Abdullah, Jafri Malin Zainuddin, Norafiza Sulong, Sarina Isa, Mohd. Nizam Jaafar, Hasnan
author_sort	Abdullah, Jafri Malin
title	Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
title_short	Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
title_full	Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
title_fullStr	Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
title_full_unstemmed	Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
title_sort	molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma
publisher	Journal of Neurosurgery Publishing Group
publishDate	2003
url	http://irep.iium.edu.my/30769/ http://irep.iium.edu.my/30769/ http://irep.iium.edu.my/30769/1/Ja_Neurosurg_Focus.pdf
first_indexed	2023-09-18T20:45:00Z
last_indexed	2023-09-18T20:45:00Z
_version_	1777409616189063168

Molecular genetic analysis of phosphatase and tensin homolog and p16 tumor suppressor genes in patients with malignant glioma

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