Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats

In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n = 6) were used and admi...

Full description

Bibliographic Details
Main Authors:	Yahya, Farhana, Mamat, Siti Syariah, Kamarolzaman, Mohammad Fauzi Fahmi, Seyedan, Atefeh Alsadat, Jakius, Krystal Fredoline, Mahmood, Nur Diyana, Shahril, Mohamad Syahmi, Suhaili, Z, Mohtarrudin, Nurhafiza, Darnis, Deny Susanti, Somchit, MN, Teh, L. K., Salleh, M. Z., Zakaria, Zainul Amiruddin
Format:	Article
Language:	English
Published:	Hindawi Publishing Corporation 2013
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/30576/ http://irep.iium.edu.my/30576/ http://irep.iium.edu.my/30576/ http://irep.iium.edu.my/30576/1/Evidence-Based_Complementary_and_Alternative_Medicine.pdf

id	iium-30576
recordtype	eprints
spelling	iium-305762013-08-05T06:29:42Z http://irep.iium.edu.my/30576/ Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyedan, Atefeh Alsadat Jakius, Krystal Fredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Z Mohtarrudin, Nurhafiza Darnis, Deny Susanti Somchit, MN Teh, L. K. Salleh, M. Z. Zakaria, Zainul Amiruddin R Medicine (General) In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n = 6) were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease (P < 0.05) in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30576/1/Evidence-Based_Complementary_and_Alternative_Medicine.pdf Yahya, Farhana and Mamat, Siti Syariah and Kamarolzaman, Mohammad Fauzi Fahmi and Seyedan, Atefeh Alsadat and Jakius, Krystal Fredoline and Mahmood, Nur Diyana and Shahril, Mohamad Syahmi and Suhaili, Z and Mohtarrudin, Nurhafiza and Darnis, Deny Susanti and Somchit, MN and Teh, L. K. and Salleh, M. Z. and Zakaria, Zainul Amiruddin (2013) Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats. Evidence-Based Complementary and Alternative Medicine, 2013. ISSN 1741-427X http://www.hindawi.com/journals/ecam/2013/636580/ 10.1155/2013/636580
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyedan, Atefeh Alsadat Jakius, Krystal Fredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Z Mohtarrudin, Nurhafiza Darnis, Deny Susanti Somchit, MN Teh, L. K. Salleh, M. Z. Zakaria, Zainul Amiruddin Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
description	In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n = 6) were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease (P < 0.05) in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation.
format	Article
author	Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyedan, Atefeh Alsadat Jakius, Krystal Fredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Z Mohtarrudin, Nurhafiza Darnis, Deny Susanti Somchit, MN Teh, L. K. Salleh, M. Z. Zakaria, Zainul Amiruddin
author_facet	Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyedan, Atefeh Alsadat Jakius, Krystal Fredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Z Mohtarrudin, Nurhafiza Darnis, Deny Susanti Somchit, MN Teh, L. K. Salleh, M. Z. Zakaria, Zainul Amiruddin
author_sort	Yahya, Farhana
title	Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
title_short	Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
title_full	Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
title_fullStr	Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
title_full_unstemmed	Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
title_sort	hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
publisher	Hindawi Publishing Corporation
publishDate	2013
url	http://irep.iium.edu.my/30576/ http://irep.iium.edu.my/30576/ http://irep.iium.edu.my/30576/ http://irep.iium.edu.my/30576/1/Evidence-Based_Complementary_and_Alternative_Medicine.pdf
first_indexed	2023-09-18T20:44:46Z
last_indexed	2023-09-18T20:44:46Z
_version_	1777409601121026048

Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats

Similar Items