Purification and cytotoxicity assay of tomato (Lycopersicon Esculentum) leaves methanol extract as potential anti-cancer agent
This research studied the cytotoxicity effect of tomato leaves methanol extract on cancer cells to address potential therapeutic in MCF-7 breast cancer cell lines and its toxicity towards Vero cells. The extraction was done in a shake flask by 82% methanol, 1:10 (w/v), agitated at 22 °C with 110...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/30512/ http://irep.iium.edu.my/30512/ http://irep.iium.edu.my/30512/1/Purification_and_Cytotoxicity_Assay.pdf |
| Summary: | This research studied the cytotoxicity effect of tomato
leaves methanol extract on cancer cells to address potential
therapeutic in MCF-7 breast cancer cell lines and its toxicity
towards Vero cells. The extraction was done in a shake flask
by 82% methanol, 1:10 (w/v), agitated at 22 °C with 110 rpm
within 24 hours. Later, purification process was started by thin
layer chromatography (TLC) subjected to determine the best
mobile phase for compound separation and collection by means
of column chromatography. Next, the effect of purified sample
towards MCF-7 breast cancer cell lines and Vero cells were
observed using in vitro cytotoxicity assay to indicate its active
fractions and its half maximal inhibitory concentration (IC50).
Purified sample gave a rational effect towards MCF-7 breast
cancer cells with IC50 value of 5.85 μg/ml compared to Taxol
with IC50 value of 0.039 μg/ml. The purified sample can also
be judged to be harmless as it has IC50 value of 765.6 μg/ml in
Vero cells treatment while Taxol gave IC50 value of 0.045 μg/
ml. |
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