Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”

The objective of the present study was to fi nd the normal values of the cytology and cytochemistry of the Pulmonary Alveolar Macrophages (PAMs) and through the identifi cation of universal normal values. Bronchoalveolar lavage (BAL) fl uid was performed by tracheal cannulation to seventy healthy adu...

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Main Authors: Salman, Mohammad O., Abd, Ali H., Al-Ani, Imad Matloub Dally, Al-Salihi, Anam R.
Format: Article
Language:English
Published: Department of Anatomy, National University of Singapore 2013
Subjects:
Online Access:http://irep.iium.edu.my/30487/
http://irep.iium.edu.my/30487/
http://irep.iium.edu.my/30487/1/Cytological_and_Cytochemical__Studies_on_the_Pulmonary_Alveolar_Macrophages.pdf
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spelling iium-304872015-12-18T02:41:19Z http://irep.iium.edu.my/30487/ Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus” Salman, Mohammad O. Abd, Ali H. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. QM Human anatomy RB Pathology The objective of the present study was to fi nd the normal values of the cytology and cytochemistry of the Pulmonary Alveolar Macrophages (PAMs) and through the identifi cation of universal normal values. Bronchoalveolar lavage (BAL) fl uid was performed by tracheal cannulation to seventy healthy adult male rats. The percentages of differential BAL cells count were 85-90% PAMs, 5-6% lymphocytes, 3-4% granulocytes and 3-4% epithelial cells. The diameter of PAMs was ranging between (14-35μ). The mean of PAMs area was 57μ2±1.03. The mean of nucleus area was 17.6μ2±1.3. The ratio of nucleus areas to cytoplasm area was1:2. The Final Reaction Product of the ANAE was homogenous brown reddish color. It was homogenous in distribution although the distribution was regular within the area of cytoplasm and nucleus of PAMs while other BAL cells showed negative reaction. 95% of total PAMs were positive in reaction. The mean of optical density of fi nal reaction product of esteratic activity in PAMs was 1.782±0.004. Department of Anatomy, National University of Singapore 2013-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30487/1/Cytological_and_Cytochemical__Studies_on_the_Pulmonary_Alveolar_Macrophages.pdf Salman, Mohammad O. and Abd, Ali H. and Al-Ani, Imad Matloub Dally and Al-Salihi, Anam R. (2013) Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”. Annals of Microscopy, 13. pp. 28-35. ISSN 0219-2209 http://www.microscopy.org.sg/journal/vol_13/Annals%20of%20Microscopy%20Vol%2013-Web.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QM Human anatomy
RB Pathology
spellingShingle QM Human anatomy
RB Pathology
Salman, Mohammad O.
Abd, Ali H.
Al-Ani, Imad Matloub Dally
Al-Salihi, Anam R.
Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”
description The objective of the present study was to fi nd the normal values of the cytology and cytochemistry of the Pulmonary Alveolar Macrophages (PAMs) and through the identifi cation of universal normal values. Bronchoalveolar lavage (BAL) fl uid was performed by tracheal cannulation to seventy healthy adult male rats. The percentages of differential BAL cells count were 85-90% PAMs, 5-6% lymphocytes, 3-4% granulocytes and 3-4% epithelial cells. The diameter of PAMs was ranging between (14-35μ). The mean of PAMs area was 57μ2±1.03. The mean of nucleus area was 17.6μ2±1.3. The ratio of nucleus areas to cytoplasm area was1:2. The Final Reaction Product of the ANAE was homogenous brown reddish color. It was homogenous in distribution although the distribution was regular within the area of cytoplasm and nucleus of PAMs while other BAL cells showed negative reaction. 95% of total PAMs were positive in reaction. The mean of optical density of fi nal reaction product of esteratic activity in PAMs was 1.782±0.004.
format Article
author Salman, Mohammad O.
Abd, Ali H.
Al-Ani, Imad Matloub Dally
Al-Salihi, Anam R.
author_facet Salman, Mohammad O.
Abd, Ali H.
Al-Ani, Imad Matloub Dally
Al-Salihi, Anam R.
author_sort Salman, Mohammad O.
title Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”
title_short Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”
title_full Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”
title_fullStr Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”
title_full_unstemmed Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”
title_sort cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “rattus rattus norvegicus albinus”
publisher Department of Anatomy, National University of Singapore
publishDate 2013
url http://irep.iium.edu.my/30487/
http://irep.iium.edu.my/30487/
http://irep.iium.edu.my/30487/1/Cytological_and_Cytochemical__Studies_on_the_Pulmonary_Alveolar_Macrophages.pdf
first_indexed 2023-09-18T20:44:38Z
last_indexed 2023-09-18T20:44:38Z
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