Purification of antibacterial compounds from spathiphyllum cannifolium leaf

Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants screened. In the present study, purification of antibacterial compou...

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Main Authors:	Ahmad Raus, Raha, Abdullah, Erlina, Jamal, Parveen, Jimat, Dzun Noraini
Format:	Article
Language:	English
Published:	2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/29936/ http://irep.iium.edu.my/29936/1/paper_Der_Pharma_Chemica.pdf

id	iium-29936
recordtype	eprints
spelling	iium-299362016-12-08T01:28:28Z http://irep.iium.edu.my/29936/ Purification of antibacterial compounds from spathiphyllum cannifolium leaf Ahmad Raus, Raha Abdullah, Erlina Jamal, Parveen Jimat, Dzun Noraini TP248.13 Biotechnology Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants screened. In the present study, purification of antibacterial compound of the plant was conducted. For purification of antibacterial compound, open column chromatography was conducted and ethyl acetate, hexane and ethanol are used as the mobile phase. The results showed that the elution of column with 80% ethyl acetate in ethanol provide fractions with the highest antibacterial activity. The fractions were analyzed with TLC plates and revealed a single spot under UV light at 0.85 Rf value. Further purification of the single spot using HPLC showed two isolated compounds at retention time of 5.53 minutes and 8.26 minutes were obtained. The compounds were suspected as flavanoids as they can be detected at wavelength 360 nm and 400 nm. 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29936/1/paper_Der_Pharma_Chemica.pdf Ahmad Raus, Raha and Abdullah, Erlina and Jamal, Parveen and Jimat, Dzun Noraini (2013) Purification of antibacterial compounds from spathiphyllum cannifolium leaf. Der Pharma Chemica, 5 (1). pp. 350-357. ISSN 0975-413X
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ahmad Raus, Raha Abdullah, Erlina Jamal, Parveen Jimat, Dzun Noraini Purification of antibacterial compounds from spathiphyllum cannifolium leaf
description	Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants screened. In the present study, purification of antibacterial compound of the plant was conducted. For purification of antibacterial compound, open column chromatography was conducted and ethyl acetate, hexane and ethanol are used as the mobile phase. The results showed that the elution of column with 80% ethyl acetate in ethanol provide fractions with the highest antibacterial activity. The fractions were analyzed with TLC plates and revealed a single spot under UV light at 0.85 Rf value. Further purification of the single spot using HPLC showed two isolated compounds at retention time of 5.53 minutes and 8.26 minutes were obtained. The compounds were suspected as flavanoids as they can be detected at wavelength 360 nm and 400 nm.
format	Article
author	Ahmad Raus, Raha Abdullah, Erlina Jamal, Parveen Jimat, Dzun Noraini
author_facet	Ahmad Raus, Raha Abdullah, Erlina Jamal, Parveen Jimat, Dzun Noraini
author_sort	Ahmad Raus, Raha
title	Purification of antibacterial compounds from spathiphyllum cannifolium leaf
title_short	Purification of antibacterial compounds from spathiphyllum cannifolium leaf
title_full	Purification of antibacterial compounds from spathiphyllum cannifolium leaf
title_fullStr	Purification of antibacterial compounds from spathiphyllum cannifolium leaf
title_full_unstemmed	Purification of antibacterial compounds from spathiphyllum cannifolium leaf
title_sort	purification of antibacterial compounds from spathiphyllum cannifolium leaf
publishDate	2013
url	http://irep.iium.edu.my/29936/ http://irep.iium.edu.my/29936/1/paper_Der_Pharma_Chemica.pdf
first_indexed	2023-09-18T20:43:56Z
last_indexed	2023-09-18T20:43:56Z
_version_	1777409549360168960

Purification of antibacterial compounds from spathiphyllum cannifolium leaf

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