Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration

Sterile filtration is considered as a final step in processing pharmaceutical grade plasmid DNA. During the development of the filtration process, fundamental understanding on the mechanism of fouling is critical to improve filtration operations. The mechanism of fouling of pQR150 (20 kb) and pGEc4...

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Main Authors:	Salim, Affaro Affandy, Eli, Keshavarz-Moore, Henk, Versteeg
Format:	Article
Language:	English
Published:	ELSEVIER 2013
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/29820/ http://irep.iium.edu.my/29820/ http://irep.iium.edu.my/29820/ http://irep.iium.edu.my/29820/1/A.Affandy-2013.pdf

id	iium-29820
recordtype	eprints
spelling	iium-298202013-05-23T03:16:47Z http://irep.iium.edu.my/29820/ Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration Salim, Affaro Affandy Eli, Keshavarz-Moore Henk, Versteeg TP155 Chemical engineering TP248.13 Biotechnology Sterile filtration is considered as a final step in processing pharmaceutical grade plasmid DNA. During the development of the filtration process, fundamental understanding on the mechanism of fouling is critical to improve filtration operations. The mechanism of fouling of pQR150 (20 kb) and pGEc47 (56 kb) plasmids DNA during constant pressure filtration inside 0.22 µm PVDF membrane is experimentally investigated. The decline of filtrate flux as function of time is analysed using the framework of classical and combined blocking models. The results for both plasmids indicate a transition between fouling mechanisms. Initially, during the early part of the filtration, the intermediate blocking model provided the best fit of the experimental results suggesting that fouling of the membrane was mainly caused by deposition of particles onto its surface. Afterwards, the result trends were best captured by the standard blocking model indicating that internal fouling of the membrane was the dominant fouling mechanism. A study of the transmission of both plasmids shows a significant reduction of plasmid transmission which coincides with the transition of the fouling mechanism from intermediate to standard blocking. The study highlights how the fouling behaviour of large plasmid DNA during sterile filtration is determined by the complex interplay between the flexibility of the molecules and the internal structure of the membrane. ELSEVIER 2013-06-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29820/1/A.Affandy-2013.pdf Salim, Affaro Affandy and Eli, Keshavarz-Moore and Henk, Versteeg (2013) Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration. Journal of Membrane Science, 437. pp. 150-159. ISSN 0376-7388 http://dx.doi.org/10.1016/j.memsci.2013.02.055 10.1016/j.memsci.2013.02.055
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Salim, Affaro Affandy Eli, Keshavarz-Moore Henk, Versteeg Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
description	Sterile filtration is considered as a final step in processing pharmaceutical grade plasmid DNA. During the development of the filtration process, fundamental understanding on the mechanism of fouling is critical to improve filtration operations. The mechanism of fouling of pQR150 (20 kb) and pGEc47 (56 kb) plasmids DNA during constant pressure filtration inside 0.22 µm PVDF membrane is experimentally investigated. The decline of filtrate flux as function of time is analysed using the framework of classical and combined blocking models. The results for both plasmids indicate a transition between fouling mechanisms. Initially, during the early part of the filtration, the intermediate blocking model provided the best fit of the experimental results suggesting that fouling of the membrane was mainly caused by deposition of particles onto its surface. Afterwards, the result trends were best captured by the standard blocking model indicating that internal fouling of the membrane was the dominant fouling mechanism. A study of the transmission of both plasmids shows a significant reduction of plasmid transmission which coincides with the transition of the fouling mechanism from intermediate to standard blocking. The study highlights how the fouling behaviour of large plasmid DNA during sterile filtration is determined by the complex interplay between the flexibility of the molecules and the internal structure of the membrane.
format	Article
author	Salim, Affaro Affandy Eli, Keshavarz-Moore Henk, Versteeg
author_facet	Salim, Affaro Affandy Eli, Keshavarz-Moore Henk, Versteeg
author_sort	Salim, Affaro Affandy
title	Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
title_short	Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
title_full	Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
title_fullStr	Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
title_full_unstemmed	Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
title_sort	application of filtration blocking models to describe fouling and transmission of large plasmids dna in sterile filtration
publisher	ELSEVIER
publishDate	2013
url	http://irep.iium.edu.my/29820/ http://irep.iium.edu.my/29820/ http://irep.iium.edu.my/29820/ http://irep.iium.edu.my/29820/1/A.Affandy-2013.pdf
first_indexed	2023-09-18T20:43:48Z
last_indexed	2023-09-18T20:43:48Z
_version_	1777409540801691648

Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration

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