Cultivable marine bacterial isolates from a sponge Hyattella cribriformis

Cultivable marine bacteria associated with the sponge Hyattella cribriformis (Hyatt, 1877) was studied through 16S rRNA gene sequencing. These marine bacterial colonies produced on Zobell Marine agar plates were distinguished based on phenotypic characters viz., colony morphology and pigmentations...

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Main Authors:	C., Prasanna Kumar, John, Akbar, Khan, Ajmal, Lyla, Nair, Yunus, Kamaruzzaman, Khan Chowdhury, Ahmed Jalal
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2013
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/29804/ http://irep.iium.edu.my/29804/ http://irep.iium.edu.my/29804/ http://irep.iium.edu.my/29804/1/Paper_2.pdf

id	iium-29804
recordtype	eprints
spelling	iium-298042017-12-08T02:37:07Z http://irep.iium.edu.my/29804/ Cultivable marine bacterial isolates from a sponge Hyattella cribriformis C., Prasanna Kumar John, Akbar Khan, Ajmal Lyla, Nair Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Q Science (General) Cultivable marine bacteria associated with the sponge Hyattella cribriformis (Hyatt, 1877) was studied through 16S rRNA gene sequencing. These marine bacterial colonies produced on Zobell Marine agar plates were distinguished based on phenotypic characters viz., colony morphology and pigmentations of the individual colonies. The extracted DNA from the individual bacterial isolates was PCR amplified using universal 16S primers and subjected to DNA sequencing. BLAST (Basic Local Alignment Tool) analysis finds that Vibrio diazotrophicus, Bacillus subtilis, B. firmus, Thalassomonas agarivorans, Oleiphilus messinensis, Planococcus maritimus and Brevundimonas vesicularis are the culturable marine bacteria associated with the sponge Hyattella cribriformis. The phylogram constructed clearly delineated the bacterial isolates into its corresponding phylum. The cultivable bacterial density of Hyattella cribriformis was found to be 65x10-5 CFU mL-1. Submitted sequences in NCBI were assigned with accession numbers (FJ834327, FJ834328, FJ834329, FJ845392, FJ845393, FJ845394 and FJ845395). It could be concluded that, application of 16S rRNA sequencing approach would yield novel insights into the diversity of bacteria associated with sponges and hence would help in exploring the commercial importance and their industrial applications. Asian Network for Scientific Information 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29804/1/Paper_2.pdf C., Prasanna Kumar and John, Akbar and Khan, Ajmal and Lyla, Nair and Yunus, Kamaruzzaman and Khan Chowdhury, Ahmed Jalal (2013) Cultivable marine bacterial isolates from a sponge Hyattella cribriformis. Journal of Biological Sciences, 13 (1). pp. 26-32. ISSN 17273048 (P), 1812-5719 (O) http://scialert.net/abstract/?doi=jbs.2013.26.32 10.3923/jbs.2013.26.32
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) C., Prasanna Kumar John, Akbar Khan, Ajmal Lyla, Nair Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Cultivable marine bacterial isolates from a sponge Hyattella cribriformis
description	Cultivable marine bacteria associated with the sponge Hyattella cribriformis (Hyatt, 1877) was studied through 16S rRNA gene sequencing. These marine bacterial colonies produced on Zobell Marine agar plates were distinguished based on phenotypic characters viz., colony morphology and pigmentations of the individual colonies. The extracted DNA from the individual bacterial isolates was PCR amplified using universal 16S primers and subjected to DNA sequencing. BLAST (Basic Local Alignment Tool) analysis finds that Vibrio diazotrophicus, Bacillus subtilis, B. firmus, Thalassomonas agarivorans, Oleiphilus messinensis, Planococcus maritimus and Brevundimonas vesicularis are the culturable marine bacteria associated with the sponge Hyattella cribriformis. The phylogram constructed clearly delineated the bacterial isolates into its corresponding phylum. The cultivable bacterial density of Hyattella cribriformis was found to be 65x10-5 CFU mL-1. Submitted sequences in NCBI were assigned with accession numbers (FJ834327, FJ834328, FJ834329, FJ845392, FJ845393, FJ845394 and FJ845395). It could be concluded that, application of 16S rRNA sequencing approach would yield novel insights into the diversity of bacteria associated with sponges and hence would help in exploring the commercial importance and their industrial applications.
format	Article
author	C., Prasanna Kumar John, Akbar Khan, Ajmal Lyla, Nair Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal
author_facet	C., Prasanna Kumar John, Akbar Khan, Ajmal Lyla, Nair Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal
author_sort	C., Prasanna Kumar
title	Cultivable marine bacterial isolates from a sponge Hyattella cribriformis
title_short	Cultivable marine bacterial isolates from a sponge Hyattella cribriformis
title_full	Cultivable marine bacterial isolates from a sponge Hyattella cribriformis
title_fullStr	Cultivable marine bacterial isolates from a sponge Hyattella cribriformis
title_full_unstemmed	Cultivable marine bacterial isolates from a sponge Hyattella cribriformis
title_sort	cultivable marine bacterial isolates from a sponge hyattella cribriformis
publisher	Asian Network for Scientific Information
publishDate	2013
url	http://irep.iium.edu.my/29804/ http://irep.iium.edu.my/29804/ http://irep.iium.edu.my/29804/ http://irep.iium.edu.my/29804/1/Paper_2.pdf
first_indexed	2023-09-18T20:43:47Z
last_indexed	2023-09-18T20:43:47Z
_version_	1777409539176398848

Cultivable marine bacterial isolates from a sponge Hyattella cribriformis

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