Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity

Purpose: To investigate the combined effect of Carica papaya extract and mineral elements wound healing. Methods: Phosphate-buffered saline (PBS) and water extracts of unripe Carica papaya. papaya were analyzed for mineral element composition (Ca, calcium; Mg, magnesium; Na, sodium; Fe, iron; Mn, ma...

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Main Authors:	Abdulrazaq, Nafiu Bidemi, Akram, Haris, Bero, Dinie Najwa, Yusoff, Mohammed, Abd Malik, Izzati, Rahman, Mohammad Tariqur
Format:	Article
Language:	English
Published:	Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2013
Subjects:	QM Human anatomy
Online Access:	http://irep.iium.edu.my/29725/ http://irep.iium.edu.my/29725/ http://irep.iium.edu.my/29725/ http://irep.iium.edu.my/29725/1/Trop_J_Pharm_Res_2013_12.pdf

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spelling	iium-297252014-09-05T08:33:05Z http://irep.iium.edu.my/29725/ Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity Abdulrazaq, Nafiu Bidemi Akram, Haris Bero, Dinie Najwa Yusoff, Mohammed Abd Malik, Izzati Rahman, Mohammad Tariqur QM Human anatomy Purpose: To investigate the combined effect of Carica papaya extract and mineral elements wound healing. Methods: Phosphate-buffered saline (PBS) and water extracts of unripe Carica papaya. papaya were analyzed for mineral element composition (Ca, calcium; Mg, magnesium; Na, sodium; Fe, iron; Mn, manganese; Zn, zinc; Ag, silver; and Se, selenium) using a microwave-assisted digestion procedure. Atomic absorption spectrophotometry was used for the analysis of the elements. Phytochemical analysis was conducted using standard protocols. Mice weighing 25 - 30 g were anesthetized, shaved and inflicted with wound at the dorsal region using a biopsy punch. The mice were treated topically twice daily with or without 5 mg/ml PBS or water extracts containing 100 or 200 μM Zn2+ or 1.0 or 0.5 μg/g Se2+. The standard (positive control) group was treated with solcoseryl and the negative control with deionised H2O. Wound area was monitored with a camera and evaluated by software. Results: PBS extract formulated with Se2+. (0.5 or 1.0 mg/g) took approximately 9 days for complete wound healing while negative control took 14 days and other treatment groups least 10 days. PBS extract contained 9 % protein but no tannin while water extract contained 0.58 % protein and tannins. Calcium (137.98 mg/100 g extract) constituted the most abundant macro-element while Se (32.14 mg/100 g extract) was the most abundant microelement in C. papaya pulp. Conclusion: Addition of selenium to C. papaya extracts augments the wound healing activity of the extracts. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2013-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29725/1/Trop_J_Pharm_Res_2013_12.pdf Abdulrazaq, Nafiu Bidemi and Akram, Haris and Bero, Dinie Najwa and Yusoff, Mohammed and Abd Malik, Izzati and Rahman, Mohammad Tariqur (2013) Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity. Tropical Journal of Pharmaceutical Research, 12 (1). pp. 77-84. ISSN 1596-9827 (O), 1596-5996 (P) http://www.ajol.info/index.php/tjpr/article/view/86181 10.4314/tjpr.v12i1.13
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QM Human anatomy
spellingShingle	QM Human anatomy Abdulrazaq, Nafiu Bidemi Akram, Haris Bero, Dinie Najwa Yusoff, Mohammed Abd Malik, Izzati Rahman, Mohammad Tariqur Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
description	Purpose: To investigate the combined effect of Carica papaya extract and mineral elements wound healing. Methods: Phosphate-buffered saline (PBS) and water extracts of unripe Carica papaya. papaya were analyzed for mineral element composition (Ca, calcium; Mg, magnesium; Na, sodium; Fe, iron; Mn, manganese; Zn, zinc; Ag, silver; and Se, selenium) using a microwave-assisted digestion procedure. Atomic absorption spectrophotometry was used for the analysis of the elements. Phytochemical analysis was conducted using standard protocols. Mice weighing 25 - 30 g were anesthetized, shaved and inflicted with wound at the dorsal region using a biopsy punch. The mice were treated topically twice daily with or without 5 mg/ml PBS or water extracts containing 100 or 200 μM Zn2+ or 1.0 or 0.5 μg/g Se2+. The standard (positive control) group was treated with solcoseryl and the negative control with deionised H2O. Wound area was monitored with a camera and evaluated by software. Results: PBS extract formulated with Se2+. (0.5 or 1.0 mg/g) took approximately 9 days for complete wound healing while negative control took 14 days and other treatment groups least 10 days. PBS extract contained 9 % protein but no tannin while water extract contained 0.58 % protein and tannins. Calcium (137.98 mg/100 g extract) constituted the most abundant macro-element while Se (32.14 mg/100 g extract) was the most abundant microelement in C. papaya pulp. Conclusion: Addition of selenium to C. papaya extracts augments the wound healing activity of the extracts.
format	Article
author	Abdulrazaq, Nafiu Bidemi Akram, Haris Bero, Dinie Najwa Yusoff, Mohammed Abd Malik, Izzati Rahman, Mohammad Tariqur
author_facet	Abdulrazaq, Nafiu Bidemi Akram, Haris Bero, Dinie Najwa Yusoff, Mohammed Abd Malik, Izzati Rahman, Mohammad Tariqur
author_sort	Abdulrazaq, Nafiu Bidemi
title	Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
title_short	Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
title_full	Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
title_fullStr	Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
title_full_unstemmed	Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
title_sort	addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity
publisher	Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
publishDate	2013
url	http://irep.iium.edu.my/29725/ http://irep.iium.edu.my/29725/ http://irep.iium.edu.my/29725/ http://irep.iium.edu.my/29725/1/Trop_J_Pharm_Res_2013_12.pdf
first_indexed	2023-09-18T20:43:41Z
last_indexed	2023-09-18T20:43:41Z
_version_	1777409533032792064

Addition of selenium to carica papaya linn pulp extract enhances dermal wound healing activity

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