Metallothionein Biosynthesis in Human RBC Precursors
The in vitro biosynthesis of metallothionein (MT) has been investigated in RBC precursors from human cord blood in order to support the hypothesis for the nucleated precursor origin of MT in human red blood cells (RBC). Human RBC precursors are obtained by (i) separating glycophorin A+ (gly A+)...
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S. Karger AG
2000
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| Online Access: | http://irep.iium.edu.my/29684/ http://irep.iium.edu.my/29684/ http://irep.iium.edu.my/29684/1/Cel_Physio_Biochem.pdf |
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iium-296842013-08-01T04:55:19Z http://irep.iium.edu.my/29684/ Metallothionein Biosynthesis in Human RBC Precursors Rahman, Mohammad Tariqur Vandingenen, Anick De Ley, Marc QM Human anatomy The in vitro biosynthesis of metallothionein (MT) has been investigated in RBC precursors from human cord blood in order to support the hypothesis for the nucleated precursor origin of MT in human red blood cells (RBC). Human RBC precursors are obtained by (i) separating glycophorin A+ (gly A+) cells using a magnetic cell sorting (MACS) technique and by (ii) ex vivo expansion of precursors BFU-E (burst forming unit-erythroid) on methylcellulose semi-solid culture media from mononuclear cells of cord blood. Biosynthesis of MT is detected at the protein level, by immuno-histochemical staining using a mouse monoclonal antibody (E9) in ex vivo expanded RBC precursors obtained from BFU-E. Expression of MT is also detected at the mRNA level by MT specific reverse transcriptase polymerase chain reaction (RT-PCR) both in ex vivo expanded precursors from BFU-E and in MACS separated gly A+ cells. In addition, the expression of the fetal form of MT, MT-0 (also known as MT-1H) at the mRNA level in glycophorin A+ cells, is also confirmed by cDNA sequencing. With these observations, to our knowledge, MT biosynthesis in human erythroid precursors is reported for the first time. Moreover, the current findings of MT-0 expression at the mRNA level in gly A+ RBC precursors of hCB has added one more member in the list of cells/organs like fetal liver, human monocytes, non-neoplastic tissues of adenocarcinoma etc., in which the expression of the human fetal form of MT, i.e. MT-0, has also been reported. S. Karger AG 2000 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29684/1/Cel_Physio_Biochem.pdf Rahman, Mohammad Tariqur and Vandingenen, Anick and De Ley, Marc (2000) Metallothionein Biosynthesis in Human RBC Precursors. Cellular Physiology and Biochemistry, 10 (4). pp. 237-242. ISSN 1015-8987 http://www.karger.com/Article/Abstract/16355 |
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Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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Online Access |
| language |
English |
| topic |
QM Human anatomy |
| spellingShingle |
QM Human anatomy Rahman, Mohammad Tariqur Vandingenen, Anick De Ley, Marc Metallothionein Biosynthesis in Human RBC Precursors |
| description |
The in vitro biosynthesis of metallothionein (MT) has
been investigated in RBC precursors from human cord
blood in order to support the hypothesis for the
nucleated precursor origin of MT in human red blood
cells (RBC). Human RBC precursors are obtained by
(i) separating glycophorin A+ (gly A+) cells using a
magnetic cell sorting (MACS) technique and by (ii) ex
vivo expansion of precursors BFU-E (burst forming
unit-erythroid) on methylcellulose semi-solid culture
media from mononuclear cells of cord blood.
Biosynthesis of MT is detected at the protein level, by
immuno-histochemical staining using a mouse
monoclonal antibody (E9) in ex vivo expanded RBC
precursors obtained from BFU-E. Expression of MT is
also detected at the mRNA level by MT specific reverse
transcriptase polymerase chain reaction (RT-PCR)
both in ex vivo expanded precursors from BFU-E and
in MACS separated gly A+ cells. In addition, the expression of the fetal form of MT, MT-0 (also known
as MT-1H) at the mRNA level in glycophorin A+ cells,
is also confirmed by cDNA sequencing. With these
observations, to our knowledge, MT biosynthesis in
human erythroid precursors is reported for the first time.
Moreover, the current findings of MT-0 expression at
the mRNA level in gly A+ RBC precursors of hCB has
added one more member in the list of cells/organs like
fetal liver, human monocytes, non-neoplastic tissues
of adenocarcinoma etc., in which the expression of the
human fetal form of MT, i.e. MT-0, has also been
reported. |
| format |
Article |
| author |
Rahman, Mohammad Tariqur Vandingenen, Anick De Ley, Marc |
| author_facet |
Rahman, Mohammad Tariqur Vandingenen, Anick De Ley, Marc |
| author_sort |
Rahman, Mohammad Tariqur |
| title |
Metallothionein Biosynthesis in Human RBC
Precursors |
| title_short |
Metallothionein Biosynthesis in Human RBC
Precursors |
| title_full |
Metallothionein Biosynthesis in Human RBC
Precursors |
| title_fullStr |
Metallothionein Biosynthesis in Human RBC
Precursors |
| title_full_unstemmed |
Metallothionein Biosynthesis in Human RBC
Precursors |
| title_sort |
metallothionein biosynthesis in human rbc
precursors |
| publisher |
S. Karger AG |
| publishDate |
2000 |
| url |
http://irep.iium.edu.my/29684/ http://irep.iium.edu.my/29684/ http://irep.iium.edu.my/29684/1/Cel_Physio_Biochem.pdf |
| first_indexed |
2023-09-18T20:43:36Z |
| last_indexed |
2023-09-18T20:43:36Z |
| _version_ |
1777409528146427904 |