Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells

Type 2 diabetes is a metabolic disorder characterized by insulin resistance, insulin action and caused by multifactorial etiology, including environmental factors, particularly diet and genetic components. Recently, most research on diabetes has focus on adipocyte which is used as a model for testin...

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Main Authors:	Bakhtiar, M. Taher, Mohamed Amiroudine, Mohamed Zaffar Ali, Darnis, Deny Susanti, Ichwan, Solachuddin J. A., Kaderi, Mohd Arifin
Format:	Conference or Workshop Item
Language:	English English
Published:	2013
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/29514/ http://irep.iium.edu.my/29514/ http://irep.iium.edu.my/29514/1/KOP.docx http://irep.iium.edu.my/29514/3/1368_Taher.pdf

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recordtype	eprints
spelling	iium-295142015-09-18T00:17:44Z http://irep.iium.edu.my/29514/ Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin RS Pharmacy and materia medica Type 2 diabetes is a metabolic disorder characterized by insulin resistance, insulin action and caused by multifactorial etiology, including environmental factors, particularly diet and genetic components. Recently, most research on diabetes has focus on adipocyte which is used as a model for testing of insulin sensitivity and novel antidiabetic drugs. In this study, we investigated the effects of cycloartane triterpenoid on the adipocyte differentiation and glucose uptake in 3T3-L1 cells together with its underlying gene expression. The cells were treated for triglyceride accumulation with different concentration of the compound using Oil Red O staining assay. After 8-days, morphological changes and increase lipid accumulation were observed in these cells (p<0.05). Indeed, the intracellular lipid accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. Analysis of insulin-induced 2-deoxy-D-[3H] glucose uptake activities shows that cycloartane triterpenoids significantly (p<0.01) improved the glucose uptake with increasing the concentration of the compounds as compared to the basal. Further evaluation with the quantitative real time polymerase chain reaction (qRT-PCR) shows that mature 3T3-L1 cells treated with cycloart-24-en-3β-ol enhance pparγ and glut4 gene expression. As a result, it demonstrated that cycloartane triterpenoids enhance pparγ and glut4 gene expression. Taken together, these results suggest that cycloart-24-en-3β-ol derived from Garcinia malaccencis could improve insulin sensitivity through the activation of pparγ as a ligand and glut4. 2013 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/29514/1/KOP.docx application/pdf en http://irep.iium.edu.my/29514/3/1368_Taher.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Darnis, Deny Susanti and Ichwan, Solachuddin J. A. and Kaderi, Mohd Arifin (2013) Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells. In: IIUM Research, Invention and Innovation Exhibition 2013 (IRIIE 2013), 19-20 Feb 2013, Gombak, Kuala Lumpur. http://www.iium.edu.my/irie/13/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells
description	Type 2 diabetes is a metabolic disorder characterized by insulin resistance, insulin action and caused by multifactorial etiology, including environmental factors, particularly diet and genetic components. Recently, most research on diabetes has focus on adipocyte which is used as a model for testing of insulin sensitivity and novel antidiabetic drugs. In this study, we investigated the effects of cycloartane triterpenoid on the adipocyte differentiation and glucose uptake in 3T3-L1 cells together with its underlying gene expression. The cells were treated for triglyceride accumulation with different concentration of the compound using Oil Red O staining assay. After 8-days, morphological changes and increase lipid accumulation were observed in these cells (p<0.05). Indeed, the intracellular lipid accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. Analysis of insulin-induced 2-deoxy-D-[3H] glucose uptake activities shows that cycloartane triterpenoids significantly (p<0.01) improved the glucose uptake with increasing the concentration of the compounds as compared to the basal. Further evaluation with the quantitative real time polymerase chain reaction (qRT-PCR) shows that mature 3T3-L1 cells treated with cycloart-24-en-3β-ol enhance pparγ and glut4 gene expression. As a result, it demonstrated that cycloartane triterpenoids enhance pparγ and glut4 gene expression. Taken together, these results suggest that cycloart-24-en-3β-ol derived from Garcinia malaccencis could improve insulin sensitivity through the activation of pparγ as a ligand and glut4.
format	Conference or Workshop Item
author	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin
author_facet	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin
author_sort	Bakhtiar, M. Taher
title	Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells
title_short	Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells
title_full	Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells
title_fullStr	Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells
title_full_unstemmed	Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells
title_sort	insulin mimicking activities of cycloartane triterpenoid in 3t3-l1 cells
publishDate	2013
url	http://irep.iium.edu.my/29514/ http://irep.iium.edu.my/29514/ http://irep.iium.edu.my/29514/1/KOP.docx http://irep.iium.edu.my/29514/3/1368_Taher.pdf
first_indexed	2023-09-18T20:43:20Z
last_indexed	2023-09-18T20:43:20Z
_version_	1777409510867992576

Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells

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