Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes

The increasing incidence of obesity has opened the gap to search for the adipogenic differentiation-inhibitory compounds from natural products. This has raised our concern to evaluate the effects of Garcinia species which is well-known for its phytochemical contents such as flavonoids, phenolic acid...

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Main Authors:	Bakhtiar, M. Taher, Mohamed Amiroudine, Mohamed Zaffar Ali, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari, Kaderi, Mohd Arifin
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/29513/ http://irep.iium.edu.my/29513/ http://irep.iium.edu.my/29513/1/1366_Taher.pdf

id	iium-29513
recordtype	eprints
spelling	iium-295132014-12-09T01:00:39Z http://irep.iium.edu.my/29513/ Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin RS Pharmacy and materia medica The increasing incidence of obesity has opened the gap to search for the adipogenic differentiation-inhibitory compounds from natural products. This has raised our concern to evaluate the effects of Garcinia species which is well-known for its phytochemical contents such as flavonoids, phenolic acids and xanthones. Despite its highest medical properties, there was no reported data for obesity prevention. In this study, we used α-mangostin, the major xanthone compounds in Garcinia malaccensis Hk.f (locally known as “manggis burung”). Firstly, we elucidated the inhibitory effect of the compounds on lipid accumulation of 3T3-L1 preadipocytes by using Oil red O staining. α-mangostin dose-dependently reduced the triglyceride accumulation on 3T3-L1 cells. All compounds showed high lipid inhibition activity at 50 μg/mL concentration (P < 0.05) compared to MDI treated cells. Besides, insulin-induced 2-deoxy-D-[3H] glucose uptake activities were significantly improved with increasing the concentration of the test compounds. Moreover, the data was significant (P > 0.05) with MDI and metformin which were used as the positive control for the assay. In addition, analysis by using the adipolysis kit shows that α-mangostin increases the amount of free fatty acid (FFA) release from the cells into the medium. Further evaluation with the quantitative real time polymerase chain reaction (qRT-PCR) shows that α-mangostin reduced the expression of pparγ genes during adipocyte differentiation. At the same time, induction of glucose uptake and free fatty acid release by α-mangostin was accompanied by the increased mRNA expression of glut4 and leptin genes. As a result, we demonstrated that α-mangostin reduce lipid accumulation with decreased pparγ expression as well as stimulate the glucose uptake and free fatty acid release from the cells via glut4 and leptin expression. In conclusion, these results indicated that α-mangostin derived from Garcinia malaccencis may be a candidate for preventing metabolic disorders such as obesity. 2013 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/29513/1/1366_Taher.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Kaderi, Mohd Arifin (2013) Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes. In: IIUM Research, Innovation and Invention Exhibition 2010 (IRIIE 2010), 26 - 27 January 2010, IIUM, Kuala Lumpur. http://www.iium.edu.my/irie/13/s13/author/list_p.php?s=name
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
description	The increasing incidence of obesity has opened the gap to search for the adipogenic differentiation-inhibitory compounds from natural products. This has raised our concern to evaluate the effects of Garcinia species which is well-known for its phytochemical contents such as flavonoids, phenolic acids and xanthones. Despite its highest medical properties, there was no reported data for obesity prevention. In this study, we used α-mangostin, the major xanthone compounds in Garcinia malaccensis Hk.f (locally known as “manggis burung”). Firstly, we elucidated the inhibitory effect of the compounds on lipid accumulation of 3T3-L1 preadipocytes by using Oil red O staining. α-mangostin dose-dependently reduced the triglyceride accumulation on 3T3-L1 cells. All compounds showed high lipid inhibition activity at 50 μg/mL concentration (P < 0.05) compared to MDI treated cells. Besides, insulin-induced 2-deoxy-D-[3H] glucose uptake activities were significantly improved with increasing the concentration of the test compounds. Moreover, the data was significant (P > 0.05) with MDI and metformin which were used as the positive control for the assay. In addition, analysis by using the adipolysis kit shows that α-mangostin increases the amount of free fatty acid (FFA) release from the cells into the medium. Further evaluation with the quantitative real time polymerase chain reaction (qRT-PCR) shows that α-mangostin reduced the expression of pparγ genes during adipocyte differentiation. At the same time, induction of glucose uptake and free fatty acid release by α-mangostin was accompanied by the increased mRNA expression of glut4 and leptin genes. As a result, we demonstrated that α-mangostin reduce lipid accumulation with decreased pparγ expression as well as stimulate the glucose uptake and free fatty acid release from the cells via glut4 and leptin expression. In conclusion, these results indicated that α-mangostin derived from Garcinia malaccencis may be a candidate for preventing metabolic disorders such as obesity.
format	Conference or Workshop Item
author	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin
author_facet	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin
author_sort	Bakhtiar, M. Taher
title	Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
title_short	Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
title_full	Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
title_fullStr	Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
title_full_unstemmed	Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
title_sort	antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes
publishDate	2013
url	http://irep.iium.edu.my/29513/ http://irep.iium.edu.my/29513/ http://irep.iium.edu.my/29513/1/1366_Taher.pdf
first_indexed	2023-09-18T20:43:20Z
last_indexed	2023-09-18T20:43:20Z
_version_	1777409510716997632

Antiobesity effects of α-mangostin through glut4 and leptin expression together with pparγ activation in adipocytes

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