Interaction between phosphoprotein and nucleocapsid of newcastle disease virus

The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role...

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Main Authors:	Ahmad Raus, Raha, Tan, Wen Siang, Ali, Abdul Manaf, Yusoff, Khatijah
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	Q Science (General) QR355 Virology
Online Access:	http://irep.iium.edu.my/29281/ http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf

id	iium-29281
recordtype	eprints
spelling	iium-292812013-06-25T11:17:26Z http://irep.iium.edu.my/29281/ Interaction between phosphoprotein and nucleocapsid of newcastle disease virus Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah Q Science (General) QR355 Virology The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role in genomic RNA synthesis by binding to the ribonucleoprotein template which composed of genomic RNA in complex with the NP. In this study, the P region of Newcastle disease virus (NDV) that binds to assembled NP (NPNC) was mapped. A total of 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes containing [35S]-methionine. These radiolabelled mutants were mixed with the purified full-length NP and determined their interactions in immunoprecipitation assay and protein binding assay. Immunoprecipitation assay showed that the region of P that binds to NPNC is located within the internal region of C-terminal half of P, from amino acids 243 to 279. Protein binding assay also showed a similar interactive domain except that the interactive region is slightly bigger (amino acids 224-279). Nevertheless, both assays showed that the Nterminal half and immediate C-terminal end of P is not involved in the binding of P to NPNC. 2009 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf Ahmad Raus, Raha and Tan, Wen Siang and Ali, Abdul Manaf and Yusoff, Khatijah (2009) Interaction between phosphoprotein and nucleocapsid of newcastle disease virus. In: Malaysian International Conference on Trends in Bioprocess Engineering., 12 - 13 December 2009, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QR355 Virology
spellingShingle	Q Science (General) QR355 Virology Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
description	The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role in genomic RNA synthesis by binding to the ribonucleoprotein template which composed of genomic RNA in complex with the NP. In this study, the P region of Newcastle disease virus (NDV) that binds to assembled NP (NPNC) was mapped. A total of 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes containing [35S]-methionine. These radiolabelled mutants were mixed with the purified full-length NP and determined their interactions in immunoprecipitation assay and protein binding assay. Immunoprecipitation assay showed that the region of P that binds to NPNC is located within the internal region of C-terminal half of P, from amino acids 243 to 279. Protein binding assay also showed a similar interactive domain except that the interactive region is slightly bigger (amino acids 224-279). Nevertheless, both assays showed that the Nterminal half and immediate C-terminal end of P is not involved in the binding of P to NPNC.
format	Conference or Workshop Item
author	Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah
author_facet	Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah
author_sort	Ahmad Raus, Raha
title	Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
title_short	Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
title_full	Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
title_fullStr	Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
title_full_unstemmed	Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
title_sort	interaction between phosphoprotein and nucleocapsid of newcastle disease virus
publishDate	2009
url	http://irep.iium.edu.my/29281/ http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf
first_indexed	2023-09-18T20:42:57Z
last_indexed	2023-09-18T20:42:57Z
_version_	1777409487536128000

Interaction between phosphoprotein and nucleocapsid of newcastle disease virus

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