Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29281/ http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf |
| Summary: | The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA
replication and transcription. In genome replication, the P binds to the unassembled
nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic
RNA. P also plays a supplemental role in genomic RNA synthesis by binding to the
ribonucleoprotein template which composed of genomic RNA in complex with the NP.
In this study, the P region of Newcastle disease virus (NDV) that binds to assembled NP
(NPNC) was mapped. A total of 18 N- and C-terminally truncated P mutants were
synthesized by in vitro translation in rabbit reticulocytes containing [35S]-methionine.
These radiolabelled mutants were mixed with the purified full-length NP and determined
their interactions in immunoprecipitation assay and protein binding assay.
Immunoprecipitation assay showed that the region of P that binds to NPNC is located
within the internal region of C-terminal half of P, from amino acids 243 to 279. Protein
binding assay also showed a similar interactive domain except that the interactive region
is slightly bigger (amino acids 224-279). Nevertheless, both assays showed that the Nterminal
half and immediate C-terminal end of P is not involved in the binding of P to
NPNC. |
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