Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia

Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia

Background: The adaptive power of Staphylococcus aureus to antibiotics lead to the emergence of methicillin-resistant S. aureus (MRSA) in the early 1960s. The cause of resistance to methicillin is the acquisition of the mecA gene, situated on a mobile genetic element called the staphylococcal casset...

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Main Authors: Mustafa, M. I, Alarosi, N. A., Mohammad Amjad, Nasser
Format: Article
Language:English
Published: Elsevier 2012
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/29251/
http://irep.iium.edu.my/29251/
http://irep.iium.edu.my/29251/
http://irep.iium.edu.my/29251/1/1-s2.0-S1201971212007461-main.pdf
id iium-29251
recordtype eprints
spelling iium-292512013-10-04T07:48:49Z http://irep.iium.edu.my/29251/ Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia Mustafa, M. I Alarosi, N. A. Mohammad Amjad, Nasser R Medicine (General) Background: The adaptive power of Staphylococcus aureus to antibiotics lead to the emergence of methicillin-resistant S. aureus (MRSA) in the early 1960s. The cause of resistance to methicillin is the acquisition of the mecA gene, situated on a mobile genetic element called the staphylococcal cassette chromosome mec (SCCmec). Methods: One of the most important techniques used to investigate the molecular epidemiology of S. aureus is SCCmec typing. This technique has been used to study the evolution of the MRSA and to study their subsequent worldwide dissemination. Twenty-eight MRSA isolates were subjected toSCCmec typing by duplex real time PCR. The MRSAs were isolated by the Bacteriology Laboratory in Hospital Tengku Ampuan Afzan (HTAA), Kuantan, Pahang, Malaysia from inpatients admitted during the period from 1stApril to 30th September, 2010. The MRSA isolates were re-identified by known bacteriological methods and the minimal inhibitory concentration (MIC) of oxacillin was determined by E-test. The antibiotic susceptibility was tested, by disc diffusion method to 7 different antibiotics. Results: Four major sites were listed (Fig 3): skin and soft tissue infections 14 (50%), ENT infections 7 (25%), respiratory tract infections 1 (3.57%), blood stream infections 5 (17.85%), and other body fluids 1 (3.57%).Resistance to oxacillin was 100%, with an MIC >4 μg/mL. Resistance to other antibacterial drugs was erythromycin 82.1%, gentamicin 75%, tetracycline 78.6%, and trimethoprim-sulfamethoxazole 78.6%. None of the isolates was resistant to vancomycin or chloramphenicol. 78.5% (22/28) were shown to be of SCCmec-type III and 21.5% (6/28) were of type IV. Conclusion: The results confirm observations in several other neighboring Far Eastern countries and corroborates the epidemicity of these two SCCmec types in Kuantan, Malaysia. Elsevier 2012-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29251/1/1-s2.0-S1201971212007461-main.pdf Mustafa, M. I and Alarosi, N. A. and Mohammad Amjad, Nasser (2012) Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia. International Journal of Infectious Diseases, 16 (supp 1). e426. ISSN 1201-9712 http://www.sciencedirect.com/science/article/pii/S1201971212007461 10.1016/j.ijid.2012.05.594
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Mustafa, M. I
Alarosi, N. A.
Mohammad Amjad, Nasser
Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia
description Background: The adaptive power of Staphylococcus aureus to antibiotics lead to the emergence of methicillin-resistant S. aureus (MRSA) in the early 1960s. The cause of resistance to methicillin is the acquisition of the mecA gene, situated on a mobile genetic element called the staphylococcal cassette chromosome mec (SCCmec). Methods: One of the most important techniques used to investigate the molecular epidemiology of S. aureus is SCCmec typing. This technique has been used to study the evolution of the MRSA and to study their subsequent worldwide dissemination. Twenty-eight MRSA isolates were subjected toSCCmec typing by duplex real time PCR. The MRSAs were isolated by the Bacteriology Laboratory in Hospital Tengku Ampuan Afzan (HTAA), Kuantan, Pahang, Malaysia from inpatients admitted during the period from 1stApril to 30th September, 2010. The MRSA isolates were re-identified by known bacteriological methods and the minimal inhibitory concentration (MIC) of oxacillin was determined by E-test. The antibiotic susceptibility was tested, by disc diffusion method to 7 different antibiotics. Results: Four major sites were listed (Fig 3): skin and soft tissue infections 14 (50%), ENT infections 7 (25%), respiratory tract infections 1 (3.57%), blood stream infections 5 (17.85%), and other body fluids 1 (3.57%).Resistance to oxacillin was 100%, with an MIC >4 μg/mL. Resistance to other antibacterial drugs was erythromycin 82.1%, gentamicin 75%, tetracycline 78.6%, and trimethoprim-sulfamethoxazole 78.6%. None of the isolates was resistant to vancomycin or chloramphenicol. 78.5% (22/28) were shown to be of SCCmec-type III and 21.5% (6/28) were of type IV. Conclusion: The results confirm observations in several other neighboring Far Eastern countries and corroborates the epidemicity of these two SCCmec types in Kuantan, Malaysia.
format Article
author Mustafa, M. I
Alarosi, N. A.
Mohammad Amjad, Nasser
author_facet Mustafa, M. I
Alarosi, N. A.
Mohammad Amjad, Nasser
author_sort Mustafa, M. I
title Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia
title_short Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia
title_full Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia
title_fullStr Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia
title_full_unstemmed Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia
title_sort staphylococcal cassette chroromosome mec (sccmec) typing of methicillin-resistant staphylococcus aureus (mrsa) isolates from patients attending tengku ampuan afzan hospital (htaa) in kuantan, pahang, malaysia
publisher Elsevier
publishDate 2012
url http://irep.iium.edu.my/29251/
http://irep.iium.edu.my/29251/
http://irep.iium.edu.my/29251/
http://irep.iium.edu.my/29251/1/1-s2.0-S1201971212007461-main.pdf
first_indexed 2023-09-18T20:42:55Z
last_indexed 2023-09-18T20:42:55Z
_version_ 1777409484977602560

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