Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers

Due to accelerated energy consumption, enhanced function of mitochondria in tumour cells compared to normal cells is prerequisite for tumour development. Leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) regulates the expression of all mitochondrial DNA-encoded mRNAs, thus play...

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Main Authors:	Tian, Tian, Ikeda, Jun-Ichiro, Wang, Yi, Mamat, Suhana, Luo, Wenjuan, Aozasa, Katsuyuki, Morii, Eiichi
Format:	Article
Language:	English
Published:	Elsevier Inc. 2012
Subjects:	QM Human anatomy
Online Access:	http://irep.iium.edu.my/29244/ http://irep.iium.edu.my/29244/ http://irep.iium.edu.my/29244/ http://irep.iium.edu.my/29244/1/Role_of_leucine-rich_pentatricopeptide_repeat.pdf

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recordtype	eprints
spelling	iium-292442013-07-18T06:57:09Z http://irep.iium.edu.my/29244/ Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers Tian, Tian Ikeda, Jun-Ichiro Wang, Yi Mamat, Suhana Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi QM Human anatomy Due to accelerated energy consumption, enhanced function of mitochondria in tumour cells compared to normal cells is prerequisite for tumour development. Leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) regulates the expression of all mitochondrial DNA-encoded mRNAs, thus plays an important role in the mitochondrial function. LRPPRC is abundantly expressed in the side population of lung adenocarcinoma cell lines, where cancer stem cells are enriched. However, the role of LRPPRC in tumour development remained to be clarified in detail. Here, the expression of LRPPRC was examined in various types of tumours, such as lung adenocarcinoma, oesophageal squamous cell carcinoma, stomach, colon, mammary and endometrial adenocarcinoma, and lymphoma. Immunohistochemistry revealed that all kinds of examined tumours abundantly expressed LRPPRC. In contrast, surrounding non-neoplastic cells hardly expressed LRPPRC. The knocked-down expression of LRPPRC in lung adenocarcinoma cells did not affect amount of side population and activity of aldehyde dehydrogenase 1, known to be highly expressed in cancer stem cells of the lung. However, the knocked-down expression of LRPPRC reduced the abilities for anti-apoptosis, invasion and in vitro colony formation in lung adenocarcinoma, as well as Hodgkin lymphoma cells. Double staining of LRPPRC with active caspase-3 in clinical samples of lung adenocarcinoma revealed that apoptotic cells were hardly observed in LRPPRC-expressing tumours. These findings indicate that LRPPRC played an important role in tumourigenesis through the resistance to apoptosis and high invasive activity. Elsevier Inc. 2012-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29244/1/Role_of_leucine-rich_pentatricopeptide_repeat.pdf Tian, Tian and Ikeda, Jun-Ichiro and Wang, Yi and Mamat, Suhana and Luo, Wenjuan and Aozasa, Katsuyuki and Morii, Eiichi (2012) Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers. European Journal of Cancer, 48 (15). pp. 2462-2473. ISSN 1879-0852 (O), 0959-8049 (P) http://www.sciencedirect.com/science/article/pii/S0959804912000597# 10.1016/j.ejca.2012.01.018
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QM Human anatomy
spellingShingle	QM Human anatomy Tian, Tian Ikeda, Jun-Ichiro Wang, Yi Mamat, Suhana Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers
description	Due to accelerated energy consumption, enhanced function of mitochondria in tumour cells compared to normal cells is prerequisite for tumour development. Leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) regulates the expression of all mitochondrial DNA-encoded mRNAs, thus plays an important role in the mitochondrial function. LRPPRC is abundantly expressed in the side population of lung adenocarcinoma cell lines, where cancer stem cells are enriched. However, the role of LRPPRC in tumour development remained to be clarified in detail. Here, the expression of LRPPRC was examined in various types of tumours, such as lung adenocarcinoma, oesophageal squamous cell carcinoma, stomach, colon, mammary and endometrial adenocarcinoma, and lymphoma. Immunohistochemistry revealed that all kinds of examined tumours abundantly expressed LRPPRC. In contrast, surrounding non-neoplastic cells hardly expressed LRPPRC. The knocked-down expression of LRPPRC in lung adenocarcinoma cells did not affect amount of side population and activity of aldehyde dehydrogenase 1, known to be highly expressed in cancer stem cells of the lung. However, the knocked-down expression of LRPPRC reduced the abilities for anti-apoptosis, invasion and in vitro colony formation in lung adenocarcinoma, as well as Hodgkin lymphoma cells. Double staining of LRPPRC with active caspase-3 in clinical samples of lung adenocarcinoma revealed that apoptotic cells were hardly observed in LRPPRC-expressing tumours. These findings indicate that LRPPRC played an important role in tumourigenesis through the resistance to apoptosis and high invasive activity.
format	Article
author	Tian, Tian Ikeda, Jun-Ichiro Wang, Yi Mamat, Suhana Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi
author_facet	Tian, Tian Ikeda, Jun-Ichiro Wang, Yi Mamat, Suhana Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi
author_sort	Tian, Tian
title	Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers
title_short	Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers
title_full	Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers
title_fullStr	Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers
title_full_unstemmed	Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers
title_sort	role of leucine-rich pentatricopeptide repeat motif-containing protein (lrpprc) for anti-apoptosis and tumourigenesis in cancers
publisher	Elsevier Inc.
publishDate	2012
url	http://irep.iium.edu.my/29244/ http://irep.iium.edu.my/29244/ http://irep.iium.edu.my/29244/ http://irep.iium.edu.my/29244/1/Role_of_leucine-rich_pentatricopeptide_repeat.pdf
first_indexed	2023-09-18T20:42:54Z
last_indexed	2023-09-18T20:42:54Z
_version_	1777409484259328000

Role of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) for anti-apoptosis and tumourigenesis in cancers

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