Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet

Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet

Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a high-methionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the...

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Main Authors: Ku Zaifah, Norsidah, Asmadi, Ahmad Yusof, Azizi, Ayob, Othman, Faizah, Kamisah, Yusof
Format: Article
Language:English
Published: Springer Netherlands 2013
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/29200/
http://irep.iium.edu.my/29200/
http://irep.iium.edu.my/29200/
http://irep.iium.edu.my/29200/1/K.-Z._Norsidah_et_al_2013.pdf
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spelling iium-292002015-04-07T03:15:05Z http://irep.iium.edu.my/29200/ Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet Ku Zaifah, Norsidah Asmadi, Ahmad Yusof Azizi, Ayob Othman, Faizah Kamisah, Yusof R Medicine (General) Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a high-methionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the control. Groups 2-6 were fed 1 % methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 and 150 mg/kg diet) was added into the diet of groups 3, 4, 5 and 6. The rats were then killed. Palm TRF at 150 mg/kg and folate supplementation prevented the increase in plasma total homocysteine (4.14 ± 0.33 and 4.30 ± 0.26 vs 5.49 ± 0.25 mmol/L, p < 0.05) induced by a high-methionine diet. The increased heart thiobarbituric acid reactive substance in rats fed with high-methionine diet was also prevented by the supplementations of palm TRF (60 and 150 mg/kg) and folate. The high-methionine group had a lower glutathione peroxidase activity (49 ± 3 vs 69 ± 4 pmol/mg protein/min) than the control group. This reduction was reversed by palm TRF at 60 and 150 mg/kg diet (p < 0.05), but not by folate. Catalase and superoxide dismutase activities were unaffected by both methionine and vitamin supplementations. In conclusion, palm TRF was comparable to folate in reducing high-methionine diet-induced hyperhomocysteinemia and oxidative stress in the rats' hearts. However, palm TRF was more effective than folate in preserving the heart glutathione peroxidase enzyme activity. Springer Netherlands 2013-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29200/1/K.-Z._Norsidah_et_al_2013.pdf Ku Zaifah, Norsidah and Asmadi, Ahmad Yusof and Azizi, Ayob and Othman, Faizah and Kamisah, Yusof (2013) Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet. Journal of Physiology and Biochemistry, 69 (3). pp. 441-449. ISSN 1138-7548 (In Press) http://link.springer.com/article/10.1007%2Fs13105-012-0226-3 10.1007/s13105-012-0226-3
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Ku Zaifah, Norsidah
Asmadi, Ahmad Yusof
Azizi, Ayob
Othman, Faizah
Kamisah, Yusof
Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
description Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a high-methionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the control. Groups 2-6 were fed 1 % methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 and 150 mg/kg diet) was added into the diet of groups 3, 4, 5 and 6. The rats were then killed. Palm TRF at 150 mg/kg and folate supplementation prevented the increase in plasma total homocysteine (4.14 ± 0.33 and 4.30 ± 0.26 vs 5.49 ± 0.25 mmol/L, p < 0.05) induced by a high-methionine diet. The increased heart thiobarbituric acid reactive substance in rats fed with high-methionine diet was also prevented by the supplementations of palm TRF (60 and 150 mg/kg) and folate. The high-methionine group had a lower glutathione peroxidase activity (49 ± 3 vs 69 ± 4 pmol/mg protein/min) than the control group. This reduction was reversed by palm TRF at 60 and 150 mg/kg diet (p < 0.05), but not by folate. Catalase and superoxide dismutase activities were unaffected by both methionine and vitamin supplementations. In conclusion, palm TRF was comparable to folate in reducing high-methionine diet-induced hyperhomocysteinemia and oxidative stress in the rats' hearts. However, palm TRF was more effective than folate in preserving the heart glutathione peroxidase enzyme activity.
format Article
author Ku Zaifah, Norsidah
Asmadi, Ahmad Yusof
Azizi, Ayob
Othman, Faizah
Kamisah, Yusof
author_facet Ku Zaifah, Norsidah
Asmadi, Ahmad Yusof
Azizi, Ayob
Othman, Faizah
Kamisah, Yusof
author_sort Ku Zaifah, Norsidah
title Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
title_short Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
title_full Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
title_fullStr Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
title_full_unstemmed Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
title_sort palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
publisher Springer Netherlands
publishDate 2013
url http://irep.iium.edu.my/29200/
http://irep.iium.edu.my/29200/
http://irep.iium.edu.my/29200/
http://irep.iium.edu.my/29200/1/K.-Z._Norsidah_et_al_2013.pdf
first_indexed 2023-09-18T20:42:51Z
last_indexed 2023-09-18T20:42:51Z
_version_ 1777409480426782720

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