Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia

The oil extract of Nigella sativa seeds (NSO) was found to preserve viability of cerebellar neurons in vitro. Recent in vivo experimental study proved that NSO daily oral treatment was able to prevent memory and learning deterioration in a murine model of mild global cerebral ischemia (MGCI) that w...

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Main Authors:	Azzubaidi, Marwan Saad, Talib, Norlelawati A., Saxena, Anil Kumar, Ahmed, Qamar Uddin, Al-Ani, Imad Matloub Dally
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	QP Physiology R Medicine (General) RB Pathology RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/29175/ http://irep.iium.edu.my/29175/ http://irep.iium.edu.my/29175/4/Preservation_of_hippocampal_ultrastructural_constituents_with_nigella_sativa_oil_treatment_to_rats_challenged_by_global_cerebral_oligemia.pdf

id	iium-29175
recordtype	eprints
spelling	iium-291752015-12-18T03:02:04Z http://irep.iium.edu.my/29175/ Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Ahmed, Qamar Uddin Al-Ani, Imad Matloub Dally QP Physiology R Medicine (General) RB Pathology RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action The oil extract of Nigella sativa seeds (NSO) was found to preserve viability of cerebellar neurons in vitro. Recent in vivo experimental study proved that NSO daily oral treatment was able to prevent memory and learning deterioration in a murine model of mild global cerebral ischemia (MGCI) that was created with permanent bilateral occlusion of the common carotid arteries (2VO) in rats. The current study’s object ive was to assess the effect of NSO treatment on pyramidal and other neighboring neurons within CA1 hippocampal subfield of rats with MGCI that was achieved through 2VO procedure. The assessment was ultrastructural using transmission electron microscopy (TEM). Nine rats were equally divided into three groups: sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment). By the end of the 10th postoperative week coronal sections of the hippocampus were collected and processed for transmission electron microscopy (TEM). The neurodegenerative changes observed in pyramidal cells, astrocytes, endothelial basement membrane and axoplasms of CA1 hippocampal region of 2VO group were completely absent in sham operated control group. Meanwhile, NSO treated groups were almost free of ultrastructural neurodegenerative changes as those detected in untreated 2VO group. This adds another supporting evidence of the promising neuroprotective activity provided by NSO treatment to prevent age related neurodegenerative diseases especially those associated with chronic global cerebral hypoperfusion such as Alzheimer’s disease. 2013-02-20 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/29175/4/Preservation_of_hippocampal_ultrastructural_constituents_with_nigella_sativa_oil_treatment_to_rats_challenged_by_global_cerebral_oligemia.pdf Azzubaidi, Marwan Saad and Talib, Norlelawati A. and Saxena, Anil Kumar and Ahmed, Qamar Uddin and Al-Ani, Imad Matloub Dally (2013) Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2013, 19-20 Feb 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM. http://www.iium.edu.my/irie/13/index.php/8-iriie/1-iium-research-invention-and-innovation-exhibition-2013
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RB Pathology RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action
spellingShingle	QP Physiology R Medicine (General) RB Pathology RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Ahmed, Qamar Uddin Al-Ani, Imad Matloub Dally Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia
description	The oil extract of Nigella sativa seeds (NSO) was found to preserve viability of cerebellar neurons in vitro. Recent in vivo experimental study proved that NSO daily oral treatment was able to prevent memory and learning deterioration in a murine model of mild global cerebral ischemia (MGCI) that was created with permanent bilateral occlusion of the common carotid arteries (2VO) in rats. The current study’s object ive was to assess the effect of NSO treatment on pyramidal and other neighboring neurons within CA1 hippocampal subfield of rats with MGCI that was achieved through 2VO procedure. The assessment was ultrastructural using transmission electron microscopy (TEM). Nine rats were equally divided into three groups: sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment). By the end of the 10th postoperative week coronal sections of the hippocampus were collected and processed for transmission electron microscopy (TEM). The neurodegenerative changes observed in pyramidal cells, astrocytes, endothelial basement membrane and axoplasms of CA1 hippocampal region of 2VO group were completely absent in sham operated control group. Meanwhile, NSO treated groups were almost free of ultrastructural neurodegenerative changes as those detected in untreated 2VO group. This adds another supporting evidence of the promising neuroprotective activity provided by NSO treatment to prevent age related neurodegenerative diseases especially those associated with chronic global cerebral hypoperfusion such as Alzheimer’s disease.
format	Conference or Workshop Item
author	Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Ahmed, Qamar Uddin Al-Ani, Imad Matloub Dally
author_facet	Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Ahmed, Qamar Uddin Al-Ani, Imad Matloub Dally
author_sort	Azzubaidi, Marwan Saad
title	Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia
title_short	Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia
title_full	Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia
title_fullStr	Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia
title_full_unstemmed	Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia
title_sort	preservation of hippocampal ultrastructural constituents with nigella sativa oil treatment to rats challenged by global cerebral oligemia
publishDate	2013
url	http://irep.iium.edu.my/29175/ http://irep.iium.edu.my/29175/ http://irep.iium.edu.my/29175/4/Preservation_of_hippocampal_ultrastructural_constituents_with_nigella_sativa_oil_treatment_to_rats_challenged_by_global_cerebral_oligemia.pdf
first_indexed	2023-09-18T20:42:48Z
last_indexed	2023-09-18T20:42:48Z
_version_	1777409477973114880

Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia

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