Localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies
A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP an...
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Elsevier
2009
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| Online Access: | http://irep.iium.edu.my/29154/ http://irep.iium.edu.my/29154/1/paper_raha_RVSV.pdf |
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iium-29154 |
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iium-291542013-04-24T07:57:23Z http://irep.iium.edu.my/29154/ Localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies Ahmad Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Eshagi, Majid Yusoff, Khatijah QH426 Genetics QR180 Immunology QR355 Virology A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located at the C-terminal end (residues 441 to 489) of the NP protein. Two other antigenic sites were located within the N-terminal end (residues 26–121 and 122–375). This study demonstrates that the N- and C-terminal ends of the NP proteins are responsible in eliciting immune response, thus it is most likely that these ends are exposed on the NP. Elsevier 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29154/1/paper_raha_RVSV.pdf Ahmad Raus, Raha and Ali, Abdul Manaf and Tan, Wen Siang and Mohd. Salleh, Hamzah and Eshagi, Majid and Yusoff, Khatijah (2009) Localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies. Research in Veterinary Science, 86. pp. 174-182. ISSN 0034-5288 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QH426 Genetics QR180 Immunology QR355 Virology |
| spellingShingle |
QH426 Genetics QR180 Immunology QR355 Virology Ahmad Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Eshagi, Majid Yusoff, Khatijah Localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| description |
A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli.
One of the antigenic sites was located at the C-terminal end (residues 441 to 489) of the NP protein. Two other antigenic sites were located within the N-terminal end (residues 26–121 and 122–375). This study demonstrates that the N- and C-terminal ends of the NP proteins are responsible in eliciting immune response, thus it is most likely that these ends are exposed on the NP. |
| format |
Article |
| author |
Ahmad Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Eshagi, Majid Yusoff, Khatijah |
| author_facet |
Ahmad Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Eshagi, Majid Yusoff, Khatijah |
| author_sort |
Ahmad Raus, Raha |
| title |
Localization of the antigenic sites of newcastle disease
virus nucleocapsid using a panel of monoclonal antibodies |
| title_short |
Localization of the antigenic sites of newcastle disease
virus nucleocapsid using a panel of monoclonal antibodies |
| title_full |
Localization of the antigenic sites of newcastle disease
virus nucleocapsid using a panel of monoclonal antibodies |
| title_fullStr |
Localization of the antigenic sites of newcastle disease
virus nucleocapsid using a panel of monoclonal antibodies |
| title_full_unstemmed |
Localization of the antigenic sites of newcastle disease
virus nucleocapsid using a panel of monoclonal antibodies |
| title_sort |
localization of the antigenic sites of newcastle disease
virus nucleocapsid using a panel of monoclonal antibodies |
| publisher |
Elsevier |
| publishDate |
2009 |
| url |
http://irep.iium.edu.my/29154/ http://irep.iium.edu.my/29154/1/paper_raha_RVSV.pdf |
| first_indexed |
2023-09-18T20:42:46Z |
| last_indexed |
2023-09-18T20:42:46Z |
| _version_ |
1777409475827728384 |