Focused ion beam machining of cell culture dish

Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads...

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Bibliographic Details
Main Author:	Ali, Mohammad Yeakub
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	T Technology (General) TJ Mechanical engineering and machinery TS Manufactures
Online Access:	http://irep.iium.edu.my/28914/ http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf

id	iium-28914
recordtype	eprints
spelling	iium-289142018-12-05T08:30:49Z http://irep.iium.edu.my/28914/ Focused ion beam machining of cell culture dish Ali, Mohammad Yeakub T Technology (General) TJ Mechanical engineering and machinery TS Manufactures Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads of the pillars then multiply by division supported on top of the nanopillars. The nanopillar structure enabled easy subculture of the cells without conventional trypsinization where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric nanopillars of few hundred nanometer of diameter. This research presents focused ion beam (FIB) maskless sputtering technique for the fabrication of nanopillars sheet to be used in cell culture dish. Nanopore sheet on metallic materials is also machined using FIB which can be used as a master microtool for the mass production of polymer nanopillars sheets by nanoprinting. The typical diameter and height of the nanopillars are 400 nm and 2 ?m respectively. 2008 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf Ali, Mohammad Yeakub (2008) Focused ion beam machining of cell culture dish. In: ISE Seminar 2008, 3rd March 2008, Thailand. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General) TJ Mechanical engineering and machinery TS Manufactures
spellingShingle	T Technology (General) TJ Mechanical engineering and machinery TS Manufactures Ali, Mohammad Yeakub Focused ion beam machining of cell culture dish
description	Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads of the pillars then multiply by division supported on top of the nanopillars. The nanopillar structure enabled easy subculture of the cells without conventional trypsinization where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric nanopillars of few hundred nanometer of diameter. This research presents focused ion beam (FIB) maskless sputtering technique for the fabrication of nanopillars sheet to be used in cell culture dish. Nanopore sheet on metallic materials is also machined using FIB which can be used as a master microtool for the mass production of polymer nanopillars sheets by nanoprinting. The typical diameter and height of the nanopillars are 400 nm and 2 ?m respectively.
format	Conference or Workshop Item
author	Ali, Mohammad Yeakub
author_facet	Ali, Mohammad Yeakub
author_sort	Ali, Mohammad Yeakub
title	Focused ion beam machining of cell culture dish
title_short	Focused ion beam machining of cell culture dish
title_full	Focused ion beam machining of cell culture dish
title_fullStr	Focused ion beam machining of cell culture dish
title_full_unstemmed	Focused ion beam machining of cell culture dish
title_sort	focused ion beam machining of cell culture dish
publishDate	2008
url	http://irep.iium.edu.my/28914/ http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf
first_indexed	2023-09-18T20:42:28Z
last_indexed	2023-09-18T20:42:28Z
_version_	1777409456622010368

Focused ion beam machining of cell culture dish

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