Fabrication of micropillar sheet for cell culture dish

Cell culture is one of the major tools in life sciences. Conventionally, cells are placed in a flat petri dish made of glass or plastic. It is used to monitor cells behavior under controlled conditions. As the cells are strongly adhered to petri dish, an additional process of trypsinization is appl...

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Main Authors:	Ali, Mohammad Yeakub, Mohd Fuad, Nurul Hajar
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TJ Mechanical engineering and machinery TS Manufactures
Online Access:	http://irep.iium.edu.my/28907/ http://irep.iium.edu.my/28907/1/082_MTE_2011_Poster_Fabrication_of_Micro.pdf

id	iium-28907
recordtype	eprints
spelling	iium-289072013-03-27T02:52:25Z http://irep.iium.edu.my/28907/ Fabrication of micropillar sheet for cell culture dish Ali, Mohammad Yeakub Mohd Fuad, Nurul Hajar TJ Mechanical engineering and machinery TS Manufactures Cell culture is one of the major tools in life sciences. Conventionally, cells are placed in a flat petri dish made of glass or plastic. It is used to monitor cells behavior under controlled conditions. As the cells are strongly adhered to petri dish, an additional process of trypsinization is applied to remove the cells from the dish. Alternatively, by using micropillar sheet, the contact surface between the cells and the pillars is smaller than traditional two dimensional petri dish. Cells were found to grow in a different way on the micropillars because of these differences in adherence to the support materials. The micropillars structure enables the cells to be removed without any additional process where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric micropillar sheets of about 1 μm or less of the micropillar diameters. This research presents the fabrication process using focused ion beam (FIB) a maskless sputtering technique for the fabrication of micropillar sheet to be used in cell culture dish. Micropore sheet on metallic materials is also machined using FIB sputtering which can be used as a master microtool for the mass production of polymer micropillar sheets by micro/nano hot embossing. The typical diameter of the micropillars is 1 μm with 3-10 aspect ratio. This FIB based process is direct, easy to fabricate, and less expensive compared to LIGA (lithography, electroplating and molding) and lithography based techniques as reported. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/28907/1/082_MTE_2011_Poster_Fabrication_of_Micro.pdf Ali, Mohammad Yeakub and Mohd Fuad, Nurul Hajar (2011) Fabrication of micropillar sheet for cell culture dish. In: Malaysia Technology Expo (MTE 2011), 17-19 February 2011, KLCC.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TJ Mechanical engineering and machinery TS Manufactures
spellingShingle	TJ Mechanical engineering and machinery TS Manufactures Ali, Mohammad Yeakub Mohd Fuad, Nurul Hajar Fabrication of micropillar sheet for cell culture dish
description	Cell culture is one of the major tools in life sciences. Conventionally, cells are placed in a flat petri dish made of glass or plastic. It is used to monitor cells behavior under controlled conditions. As the cells are strongly adhered to petri dish, an additional process of trypsinization is applied to remove the cells from the dish. Alternatively, by using micropillar sheet, the contact surface between the cells and the pillars is smaller than traditional two dimensional petri dish. Cells were found to grow in a different way on the micropillars because of these differences in adherence to the support materials. The micropillars structure enables the cells to be removed without any additional process where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric micropillar sheets of about 1 μm or less of the micropillar diameters. This research presents the fabrication process using focused ion beam (FIB) a maskless sputtering technique for the fabrication of micropillar sheet to be used in cell culture dish. Micropore sheet on metallic materials is also machined using FIB sputtering which can be used as a master microtool for the mass production of polymer micropillar sheets by micro/nano hot embossing. The typical diameter of the micropillars is 1 μm with 3-10 aspect ratio. This FIB based process is direct, easy to fabricate, and less expensive compared to LIGA (lithography, electroplating and molding) and lithography based techniques as reported.
format	Conference or Workshop Item
author	Ali, Mohammad Yeakub Mohd Fuad, Nurul Hajar
author_facet	Ali, Mohammad Yeakub Mohd Fuad, Nurul Hajar
author_sort	Ali, Mohammad Yeakub
title	Fabrication of micropillar sheet for cell culture dish
title_short	Fabrication of micropillar sheet for cell culture dish
title_full	Fabrication of micropillar sheet for cell culture dish
title_fullStr	Fabrication of micropillar sheet for cell culture dish
title_full_unstemmed	Fabrication of micropillar sheet for cell culture dish
title_sort	fabrication of micropillar sheet for cell culture dish
publishDate	2011
url	http://irep.iium.edu.my/28907/ http://irep.iium.edu.my/28907/1/082_MTE_2011_Poster_Fabrication_of_Micro.pdf
first_indexed	2023-09-18T20:42:27Z
last_indexed	2023-09-18T20:42:27Z
_version_	1777409456041099264

Fabrication of micropillar sheet for cell culture dish

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