Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition

In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) w...

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Main Authors:	Bimakr, Mandana, Rahman, Russly, Taip, Farah Saleena, Mohd Adzahan, Noranizan, Sarker, Md. Zaidul Islam, Ganjloo, Ali
Format:	Article
Language:	English
Published:	MDPI 2013
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/28631/ http://irep.iium.edu.my/28631/ http://irep.iium.edu.my/28631/ http://irep.iium.edu.my/28631/2/Molecules-Zaidul-Mandana.pdf

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recordtype	eprints
spelling	iium-286312016-02-24T10:30:54Z http://irep.iium.edu.my/28631/ Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali QD Chemistry In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) were studied through response surface methodology (RSM). The SC-CO2 extraction process was modified using ethanol (99.9%)as co-solvent. Perturbation plot revealed the significant effect of all process variables on the CEY. A central composite design (CCD) was used to optimize the process conditions to achieve maximum CEY. The optimum conditions were 244 bar pressure, 46 °C temperature and 97 min dynamic extraction time. Under these optimal conditions, the CEY was predicted to be 176.30 mg-extract/g-dried sample. The validation experiment results agreed with the predicted value. The antioxidant activity and fatty acid composition of crude oil obtained under optimized conditions were determined and compared with published results using Soxhlet extraction (SE) and ultrasound assisted extraction (UAE). It was found that the antioxidant activity of the extract obtained by SC-CO2 extraction was strongly higher than those obtained by SE and UAE. Identification of fatty acid composition using gas chromatography (GC) showed that all the extracts were rich in unsaturated fatty acids with the most being linoleic acid. In contrast, the amount of saturated fatty acids extracted by SE was higher than that extracted under optimized SC-CO2 extraction conditions. MDPI 2013-01-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28631/2/Molecules-Zaidul-Mandana.pdf Bimakr, Mandana and Rahman, Russly and Taip, Farah Saleena and Mohd Adzahan, Noranizan and Sarker, Md. Zaidul Islam and Ganjloo, Ali (2013) Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition. Molecules, 18 (1). pp. 997-1014. ISSN 1420-3049 http://www.mdpi.com/journal/molecules doi:10.3390/molecules18010997
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
description	In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) were studied through response surface methodology (RSM). The SC-CO2 extraction process was modified using ethanol (99.9%)as co-solvent. Perturbation plot revealed the significant effect of all process variables on the CEY. A central composite design (CCD) was used to optimize the process conditions to achieve maximum CEY. The optimum conditions were 244 bar pressure, 46 °C temperature and 97 min dynamic extraction time. Under these optimal conditions, the CEY was predicted to be 176.30 mg-extract/g-dried sample. The validation experiment results agreed with the predicted value. The antioxidant activity and fatty acid composition of crude oil obtained under optimized conditions were determined and compared with published results using Soxhlet extraction (SE) and ultrasound assisted extraction (UAE). It was found that the antioxidant activity of the extract obtained by SC-CO2 extraction was strongly higher than those obtained by SE and UAE. Identification of fatty acid composition using gas chromatography (GC) showed that all the extracts were rich in unsaturated fatty acids with the most being linoleic acid. In contrast, the amount of saturated fatty acids extracted by SE was higher than that extracted under optimized SC-CO2 extraction conditions.
format	Article
author	Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali
author_facet	Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali
author_sort	Bimakr, Mandana
title	Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
title_short	Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
title_full	Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
title_fullStr	Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
title_full_unstemmed	Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
title_sort	supercritical carbon dioxide extraction of seed oil from winter melon (benincasa hispida) and its antioxidant activity and fatty acid composition
publisher	MDPI
publishDate	2013
url	http://irep.iium.edu.my/28631/ http://irep.iium.edu.my/28631/ http://irep.iium.edu.my/28631/ http://irep.iium.edu.my/28631/2/Molecules-Zaidul-Mandana.pdf
first_indexed	2023-09-18T20:42:10Z
last_indexed	2023-09-18T20:42:10Z
_version_	1777409437939531776

Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition

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