Bromelain production: Current trends and perspective
Bromelain is a mixture of proteolytic enzymes derived from the stems and juice of pineapples. It has been extensively used in food industry; for meat tenderization, baking processes, and in prevention of browning of apple juice. Stem bromelain is a highly accepted phytotherapeutic agent. It has an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Museum Hist Naturelle
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/28364/ http://irep.iium.edu.my/28364/1/Bromelain_review.pdf |
| Summary: | Bromelain is a mixture of proteolytic enzymes derived from the stems and juice of pineapples. It has been
extensively used in food industry; for meat tenderization, baking processes, and in prevention of browning
of apple juice. Stem bromelain is a highly accepted phytotherapeutic agent. It has anti-tumor and antiinflammatory
effects, use in wound healing, as digestive aids, etc. This review aims at presenting a detailed
account on the level of achievement on bromelain production from the conventional methods. Specifically,
the isolation, purification and the biochemical properties of bromelain are discussed. The study also
focused on the current trends and the progress made in the production of recombinant stem bromelain in
Escherichia coli. This involved cloning of stem bromelain gene and its expression, optimization of labscale
fermentation conditions for E. coli harboring recombinant bromelain as well as the enzyme thermal
and storage stability measured using differential scanning calorimetry. It is hoped that this review would
provide relevant information to contemporary researchers who are active in the field of bromelain and other
related proteases. |
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