Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

Background: In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities. Methods: Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of Orthosi...

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Main Authors:	Mohamed, Elsnoussi Ali Hussin, Siddiqui, Mohammad Jamshed Ahmad, Lee, Fung Ang, Sadikun, Amirin, Sue, Hay Chan, Soo , Choon Tan, Asmawi, Mohd Zaini, Mun , Fei Yam
Format:	Article
Language:	English
Published:	BioMed Central 2012
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/28353/ http://irep.iium.edu.my/28353/1/BMC_CAM-2012.pdf

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recordtype	eprints
spelling	iium-283532014-07-17T05:11:18Z http://irep.iium.edu.my/28353/ Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism Mohamed, Elsnoussi Ali Hussin Siddiqui, Mohammad Jamshed Ahmad Lee, Fung Ang Sadikun, Amirin Sue, Hay Chan Soo , Choon Tan Asmawi, Mohd Zaini Mun , Fei Yam QD Chemistry Background: In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities. Methods: Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of Orthosiphon stamineus. The structure of this pure compound was determined on the NMR data and the α-glucosidase and α-amylase inhibitory activities of isolated sinensetin and 50% ethanolic extract of Orthosiphon stamineus were evaluated. Results: In vitro studies of a 50% ethanolic extract of O. stamineus and the isolated sinensetin compound showed inhibitory activity on α-glucosidase (IC50: 4.63 and 0.66 mg/ml, respectively) and α-amylase (IC50: 36.70 mg/ml and 1.13 mg/ml, respectively). Inhibition of these enzymes provides a strong biochemical basis for the management of type 2 diabetes via the control of glucose absorption. Conclusion: Alpha-glucosidase and α-amylase inhibition could the mechanisms through which the 50% ethanolic extract of O. stamineus and sinensetin exert their antidiabetic activity, indicating that it could have potential use in the management of non-insulin-dependent diabetes BioMed Central 2012-10-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28353/1/BMC_CAM-2012.pdf Mohamed, Elsnoussi Ali Hussin and Siddiqui, Mohammad Jamshed Ahmad and Lee, Fung Ang and Sadikun, Amirin and Sue, Hay Chan and Soo , Choon Tan and Asmawi, Mohd Zaini and Mun , Fei Yam (2012) Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism. BMC Complimentary and Alternative Medicine, 12 (176). pp. 1-7. ISSN 14726882
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Mohamed, Elsnoussi Ali Hussin Siddiqui, Mohammad Jamshed Ahmad Lee, Fung Ang Sadikun, Amirin Sue, Hay Chan Soo , Choon Tan Asmawi, Mohd Zaini Mun , Fei Yam Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
description	Background: In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities. Methods: Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of Orthosiphon stamineus. The structure of this pure compound was determined on the NMR data and the α-glucosidase and α-amylase inhibitory activities of isolated sinensetin and 50% ethanolic extract of Orthosiphon stamineus were evaluated. Results: In vitro studies of a 50% ethanolic extract of O. stamineus and the isolated sinensetin compound showed inhibitory activity on α-glucosidase (IC50: 4.63 and 0.66 mg/ml, respectively) and α-amylase (IC50: 36.70 mg/ml and 1.13 mg/ml, respectively). Inhibition of these enzymes provides a strong biochemical basis for the management of type 2 diabetes via the control of glucose absorption. Conclusion: Alpha-glucosidase and α-amylase inhibition could the mechanisms through which the 50% ethanolic extract of O. stamineus and sinensetin exert their antidiabetic activity, indicating that it could have potential use in the management of non-insulin-dependent diabetes
format	Article
author	Mohamed, Elsnoussi Ali Hussin Siddiqui, Mohammad Jamshed Ahmad Lee, Fung Ang Sadikun, Amirin Sue, Hay Chan Soo , Choon Tan Asmawi, Mohd Zaini Mun , Fei Yam
author_facet	Mohamed, Elsnoussi Ali Hussin Siddiqui, Mohammad Jamshed Ahmad Lee, Fung Ang Sadikun, Amirin Sue, Hay Chan Soo , Choon Tan Asmawi, Mohd Zaini Mun , Fei Yam
author_sort	Mohamed, Elsnoussi Ali Hussin
title	Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
title_short	Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
title_full	Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
title_fullStr	Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
title_full_unstemmed	Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
title_sort	potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from orthosiphon stamineus benth as anti-diabetic mechanism
publisher	BioMed Central
publishDate	2012
url	http://irep.iium.edu.my/28353/ http://irep.iium.edu.my/28353/1/BMC_CAM-2012.pdf
first_indexed	2023-09-18T20:41:51Z
last_indexed	2023-09-18T20:41:51Z
_version_	1777409417559408640

Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

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