The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the tes...

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Main Authors:	Salman , Mohammad Oda, Al-Wasiti , Estabraq A., Thamir , Khalid A., Al-Ani, Imad Matloub Dally, Al-Salihi , Anam R.
Format:	Article
Language:	English
Published:	The Faculty of Medicine, International Islamic University Malaysia 2012
Subjects:	QL Zoology QM Human anatomy QP Physiology RB Pathology
Online Access:	http://irep.iium.edu.my/28035/ http://irep.iium.edu.my/28035/1/Vol11No2%2520Pg11-17.pdf

id	iium-28035
recordtype	eprints
spelling	iium-280352015-12-18T02:42:38Z http://irep.iium.edu.my/28035/ The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir , Khalid A. Al-Ani, Imad Matloub Dally Al-Salihi , Anam R. QL Zoology QM Human anatomy QP Physiology RB Pathology Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation. The Faculty of Medicine, International Islamic University Malaysia 2012-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28035/1/Vol11No2%2520Pg11-17.pdf Salman , Mohammad Oda and Al-Wasiti , Estabraq A. and Thamir , Khalid A. and Al-Ani, Imad Matloub Dally and Al-Salihi , Anam R. (2012) The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study. The International Medical Journal Malaysia, 11 (2). pp. 11-17. ISSN 1823-4631
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology QM Human anatomy QP Physiology RB Pathology
spellingShingle	QL Zoology QM Human anatomy QP Physiology RB Pathology Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir , Khalid A. Al-Ani, Imad Matloub Dally Al-Salihi , Anam R. The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
description	Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.
format	Article
author	Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir , Khalid A. Al-Ani, Imad Matloub Dally Al-Salihi , Anam R.
author_facet	Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir , Khalid A. Al-Ani, Imad Matloub Dally Al-Salihi , Anam R.
author_sort	Salman , Mohammad Oda
title	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_short	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_full	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_fullStr	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_full_unstemmed	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_sort	effects of vasectomy on testicular tissue of mice: histological changes and dna fragmentation study
publisher	The Faculty of Medicine, International Islamic University Malaysia
publishDate	2012
url	http://irep.iium.edu.my/28035/ http://irep.iium.edu.my/28035/1/Vol11No2%2520Pg11-17.pdf
first_indexed	2023-09-18T20:41:30Z
last_indexed	2023-09-18T20:41:30Z
_version_	1777409395471155200

The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study

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