Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood

Investigation on the total phenolic content (TPC) and free-radical scavenging activity of Nauclea subdita (Korth) Steud. were carried out. Information on these activities might reflect the potency of the plant extract as an antioxidant. Young and matured trees of N. subdita were harvested and cut in...

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Main Authors:	Jamaluddin, Fatin Ruzanna, Wahab, Razak, Mohd Daud, Jamaluddin, Rahman, Shafiqur
Format:	Article
Language:	English
Published:	AENSI 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/27905/ http://irep.iium.edu.my/27905/ http://irep.iium.edu.my/27905/1/ANAS_2012_1116-1124.pdf

id	iium-27905
recordtype	eprints
spelling	iium-279052016-02-22T07:24:21Z http://irep.iium.edu.my/27905/ Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood Jamaluddin, Fatin Ruzanna Wahab, Razak Mohd Daud, Jamaluddin Rahman, Shafiqur Q Science (General) Investigation on the total phenolic content (TPC) and free-radical scavenging activity of Nauclea subdita (Korth) Steud. were carried out. Information on these activities might reflect the potency of the plant extract as an antioxidant. Young and matured trees of N. subdita were harvested and cut into three heartwood parts (top, middle and bottom) and grounded into powdered forms. Soxhlet extraction of the heartwood samples using polar (methanol) and non-polar (hexane) solvents, and phytochemical screening tests were carried out. The total phenolic contents of the methanolic crude extracts from heartwood parts were determined by colorimetry at 760 nm using Folin-Ciocalteau’s reagent, and gallic acid was used as standard. The methanolic extracts were also checked for primary antioxidant activities using an in vitro method, measured by free-radical scavenging activity of 2, 2’-diphenyl-1- picrylhydrazyl (DPPH) radical. The absorbances were measured at 517 nm by UV/VIS spectrophotometer and compared with standards Trolox and butylated hydroxyanisole (BHA). The results from this study suggest that N. subdita heartwood extract may serve as a potential source of natural antioxidant for future development in food and nutraceutical applications. AENSI 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/27905/1/ANAS_2012_1116-1124.pdf Jamaluddin, Fatin Ruzanna and Wahab, Razak and Mohd Daud, Jamaluddin and Rahman, Shafiqur (2012) Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood. Advances in Natural and Applied Sciences, 6 (7). pp. 1116-1124. ISSN 1995-0772 http://www.aensiweb.com/anas.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Jamaluddin, Fatin Ruzanna Wahab, Razak Mohd Daud, Jamaluddin Rahman, Shafiqur Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood
description	Investigation on the total phenolic content (TPC) and free-radical scavenging activity of Nauclea subdita (Korth) Steud. were carried out. Information on these activities might reflect the potency of the plant extract as an antioxidant. Young and matured trees of N. subdita were harvested and cut into three heartwood parts (top, middle and bottom) and grounded into powdered forms. Soxhlet extraction of the heartwood samples using polar (methanol) and non-polar (hexane) solvents, and phytochemical screening tests were carried out. The total phenolic contents of the methanolic crude extracts from heartwood parts were determined by colorimetry at 760 nm using Folin-Ciocalteau’s reagent, and gallic acid was used as standard. The methanolic extracts were also checked for primary antioxidant activities using an in vitro method, measured by free-radical scavenging activity of 2, 2’-diphenyl-1- picrylhydrazyl (DPPH) radical. The absorbances were measured at 517 nm by UV/VIS spectrophotometer and compared with standards Trolox and butylated hydroxyanisole (BHA). The results from this study suggest that N. subdita heartwood extract may serve as a potential source of natural antioxidant for future development in food and nutraceutical applications.
format	Article
author	Jamaluddin, Fatin Ruzanna Wahab, Razak Mohd Daud, Jamaluddin Rahman, Shafiqur
author_facet	Jamaluddin, Fatin Ruzanna Wahab, Razak Mohd Daud, Jamaluddin Rahman, Shafiqur
author_sort	Jamaluddin, Fatin Ruzanna
title	Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood
title_short	Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood
title_full	Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood
title_fullStr	Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood
title_full_unstemmed	Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood
title_sort	total phenolic contents and free-radical scavenging activities from methanolic extracts of nauclea subdita (korth) steud. heartwood
publisher	AENSI
publishDate	2012
url	http://irep.iium.edu.my/27905/ http://irep.iium.edu.my/27905/ http://irep.iium.edu.my/27905/1/ANAS_2012_1116-1124.pdf
first_indexed	2023-09-18T20:41:20Z
last_indexed	2023-09-18T20:41:20Z
_version_	1777409385183576064

Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood

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