Cresyl violet staining to assess neuroprotective and euroregenerative effects of Haruan traditional extract against eurodegenerative damage of ketamine
Ketamine abuse is on the increase in Malaysia. Neurodegenerative change following ketamine use results in debilitating behavioural and cognitive dysfunctions. These changes are thought to be the consequences of network and cellular disorganization and degeneration in the brain involving areas such...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Sciences
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/27822/ http://irep.iium.edu.my/27822/ http://irep.iium.edu.my/27822/1/Cresyl_violet_staining_to_assess_neuroprotective.pdf |
| Summary: | Ketamine abuse is on the increase in Malaysia. Neurodegenerative change following ketamine use results in debilitating behavioural and cognitive
dysfunctions. These changes are thought to be the consequences of network and cellular disorganization and degeneration in the brain involving
areas such as hippocampus. Ketamine works by blocking NMDA receptor and hence ketamine research has value in study on NMDA-related
neurological disorders such as schizophrenia. This work study the histological change in rats brain which was exposed to ketamine and the effect of
treating ketamine-exposed rats with haruan traditional extract (HTE) a substance which has been shown to have positive neuroregenerative
function in cell culture study. The changes were studied using cresyl violet and parameters such as pathological score, dead cell count and
degenerative change were evaluated. It was demonstrated that ketamine influences these parameters in exposure length-dependent manners and
degenerative change could be ameliorated by supplementation with haruan therapeutic extract (HTE) pre-damage induction but not post-damage
induction. The result adds to the growing number of evidence on neuroplasticity, and the possible role of non-endogenous substances in protecting
as well as inducing genesis of neurons for diseases that share similar pattern of neurological change as ketamine-abused subjects. |
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