Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract

Moringa Oleifera is one of the most extensively studied plant materials for water treatment. It is usually referred to as a miracle plant because of its multipurpose uses. Several studies have revealed that it possesses antibacterial activity against harmful bacteria. This study investigates the eff...

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Main Authors:	Muyibi, Suleyman Aremu, Idris Abolore, Munirat, Jamal, Parveen, Jami, Mohammed Saedi, Abdulkarim, Mohd Ismail
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	TD201 Water supply for domestic and industrial purposes
Online Access:	http://irep.iium.edu.my/27548/ http://irep.iium.edu.my/27548/1/ICEN2012.pdf

id	iium-27548
recordtype	eprints
spelling	iium-275482013-01-02T06:05:09Z http://irep.iium.edu.my/27548/ Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract Muyibi, Suleyman Aremu Idris Abolore, Munirat Jamal, Parveen Jami, Mohammed Saedi Abdulkarim, Mohd Ismail TD201 Water supply for domestic and industrial purposes Moringa Oleifera is one of the most extensively studied plant materials for water treatment. It is usually referred to as a miracle plant because of its multipurpose uses. Several studies have revealed that it possesses antibacterial activity against harmful bacteria. This study investigates the effect of two extraction methods; aqueous extraction and 1M salt extraction of Moringa Oleifera seed extracts against Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus strains using the agar well diffusion method. The effects of various concentrations of the seed extracts against the selected bacterial strains were carried out and an attempt was made to find the Minimum Inhibitory Concentration (MIC) as well as the Minimum Bactericidal Concentration (MBC) of the extracts. The results of both extraction methods showed that Moringa Oleifera seed extracts have a strong antibacterial activity against all the bacterial strains. The diameter of inhibition zone ranges from 13mm to 30mm for the two extraction methods. However, the salt extract showed a higher inhibition of 30mm at extract concentration of 5mg/ml against all the bacterial strains. The MIC of the salt extract was obtained at 1.562mg/ml for Escherichia coli, 3.125mg/ml for Pseudosomonas aeruginosa, 1.562mg/ml for Bacillus subtilis and 3.125mg/ml for Staphylococcus aureus while the MBC for the salt extract was obtained at 6.25mg/ml, 6.25mg/ml, 12.5mg/ml, 12.5mg/ml for Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus respectively. The results of these findings show that both aqueous and salt extracts of Moringa Oleifera seed have a significant antibacterial property that can be utilized for disinfection purposes for drinking water. Keywords: Agar well diffusion, antibacterial activity, Moringa Oleifera, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/27548/1/ICEN2012.pdf Muyibi, Suleyman Aremu and Idris Abolore, Munirat and Jamal, Parveen and Jami, Mohammed Saedi and Abdulkarim, Mohd Ismail (2012) Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract. In: International Conference on Environment 2012, 11th - 13th December 2012, Penang, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TD201 Water supply for domestic and industrial purposes
spellingShingle	TD201 Water supply for domestic and industrial purposes Muyibi, Suleyman Aremu Idris Abolore, Munirat Jamal, Parveen Jami, Mohammed Saedi Abdulkarim, Mohd Ismail Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract
description	Moringa Oleifera is one of the most extensively studied plant materials for water treatment. It is usually referred to as a miracle plant because of its multipurpose uses. Several studies have revealed that it possesses antibacterial activity against harmful bacteria. This study investigates the effect of two extraction methods; aqueous extraction and 1M salt extraction of Moringa Oleifera seed extracts against Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus strains using the agar well diffusion method. The effects of various concentrations of the seed extracts against the selected bacterial strains were carried out and an attempt was made to find the Minimum Inhibitory Concentration (MIC) as well as the Minimum Bactericidal Concentration (MBC) of the extracts. The results of both extraction methods showed that Moringa Oleifera seed extracts have a strong antibacterial activity against all the bacterial strains. The diameter of inhibition zone ranges from 13mm to 30mm for the two extraction methods. However, the salt extract showed a higher inhibition of 30mm at extract concentration of 5mg/ml against all the bacterial strains. The MIC of the salt extract was obtained at 1.562mg/ml for Escherichia coli, 3.125mg/ml for Pseudosomonas aeruginosa, 1.562mg/ml for Bacillus subtilis and 3.125mg/ml for Staphylococcus aureus while the MBC for the salt extract was obtained at 6.25mg/ml, 6.25mg/ml, 12.5mg/ml, 12.5mg/ml for Escherichia coli, Pseudosomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus respectively. The results of these findings show that both aqueous and salt extracts of Moringa Oleifera seed have a significant antibacterial property that can be utilized for disinfection purposes for drinking water. Keywords: Agar well diffusion, antibacterial activity, Moringa Oleifera, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration
format	Conference or Workshop Item
author	Muyibi, Suleyman Aremu Idris Abolore, Munirat Jamal, Parveen Jami, Mohammed Saedi Abdulkarim, Mohd Ismail
author_facet	Muyibi, Suleyman Aremu Idris Abolore, Munirat Jamal, Parveen Jami, Mohammed Saedi Abdulkarim, Mohd Ismail
author_sort	Muyibi, Suleyman Aremu
title	Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract
title_short	Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract
title_full	Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract
title_fullStr	Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract
title_full_unstemmed	Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract
title_sort	investigation of extraction methods on the antibacterial activity of moringa oleifera seed extract
publishDate	2012
url	http://irep.iium.edu.my/27548/ http://irep.iium.edu.my/27548/1/ICEN2012.pdf
first_indexed	2023-09-18T20:40:54Z
last_indexed	2023-09-18T20:40:54Z
_version_	1777409358411333632

Investigation of extraction methods on the antibacterial activity of Moringa oleifera seed extract

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