Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient

The aim of the study was to elucidate the full-length characteristics of HCV genome (genotype 3) in an acute HCV infection. Serum samples were obtained from an acute patient (MAL 43) and one confirmed chronic HCV infected patient (MAL 22). Reverse transcription polymerase chain reaction (RT-PCR) was...

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Main Authors:	Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Hasmoni, Mohamed Hadzri, Seman, Ramli, Abdullah, Nasuruddin
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/27173/ http://irep.iium.edu.my/27173/1/Poster_7th_NIDSAW.pdf

id	iium-27173
recordtype	eprints
spelling	iium-271732013-04-11T05:00:58Z http://irep.iium.edu.my/27173/ Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Hasmoni, Mohamed Hadzri Seman, Ramli Abdullah, Nasuruddin R Medicine (General) The aim of the study was to elucidate the full-length characteristics of HCV genome (genotype 3) in an acute HCV infection. Serum samples were obtained from an acute patient (MAL 43) and one confirmed chronic HCV infected patient (MAL 22). Reverse transcription polymerase chain reaction (RT-PCR) was employed to amplify the conserve regions of 5’UTR and NS5B for pre-genotyping purposes. Novel primers were designed to amplify multiple segments of HCV genome. Base RNA sequences were elucidated by cloning and sequencing methods. A series of overlapping clones was generated that provided a continuous stretch of 9430 and 9429 nucleotides of MAL 22 and MAL 43 isolates, respectively. Both sequences showed a single ORF encoding a polyprotein of 3021 amino acids. In this study, three hypervariable regions (HVRs) were found in E2 region, named HVR1, HVR2 and HVR3, which HVR3 was highly represented in MAL 43. Generally, all the functional regions were well maintained in the two isolates. However, novel HVR3 in the acutely infected patient indicated that HVR3 plays an important role in developing an infection. Further studies associated with HVR3 mutation in the antibody response should be evaluated to understand the mechanism of HCV replication in a sustained HCV infection, particularly for HCV genotype 3. 2012-11-06 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/27173/1/Poster_7th_NIDSAW.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Hasmoni, Mohamed Hadzri and Seman, Ramli and Abdullah, Nasuruddin (2012) Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient. In: 7th International Infectious Diseases Seminar and Workshop (NIDSAW 2012), 6th - 8th November 2012, UPM, Serdang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Hasmoni, Mohamed Hadzri Seman, Ramli Abdullah, Nasuruddin Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient
description	The aim of the study was to elucidate the full-length characteristics of HCV genome (genotype 3) in an acute HCV infection. Serum samples were obtained from an acute patient (MAL 43) and one confirmed chronic HCV infected patient (MAL 22). Reverse transcription polymerase chain reaction (RT-PCR) was employed to amplify the conserve regions of 5’UTR and NS5B for pre-genotyping purposes. Novel primers were designed to amplify multiple segments of HCV genome. Base RNA sequences were elucidated by cloning and sequencing methods. A series of overlapping clones was generated that provided a continuous stretch of 9430 and 9429 nucleotides of MAL 22 and MAL 43 isolates, respectively. Both sequences showed a single ORF encoding a polyprotein of 3021 amino acids. In this study, three hypervariable regions (HVRs) were found in E2 region, named HVR1, HVR2 and HVR3, which HVR3 was highly represented in MAL 43. Generally, all the functional regions were well maintained in the two isolates. However, novel HVR3 in the acutely infected patient indicated that HVR3 plays an important role in developing an infection. Further studies associated with HVR3 mutation in the antibody response should be evaluated to understand the mechanism of HCV replication in a sustained HCV infection, particularly for HCV genotype 3.
format	Conference or Workshop Item
author	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Hasmoni, Mohamed Hadzri Seman, Ramli Abdullah, Nasuruddin
author_facet	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Hasmoni, Mohamed Hadzri Seman, Ramli Abdullah, Nasuruddin
author_sort	Hamzah, Hairul Aini
title	Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient
title_short	Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient
title_full	Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient
title_fullStr	Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient
title_full_unstemmed	Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient
title_sort	full-length characteristics of hepatitis c virus genotype 3 in acutely infected patient
publishDate	2012
url	http://irep.iium.edu.my/27173/ http://irep.iium.edu.my/27173/1/Poster_7th_NIDSAW.pdf
first_indexed	2023-09-18T20:40:24Z
last_indexed	2023-09-18T20:40:24Z
_version_	1777409326242070528

Full-length characteristics of hepatitis C virus genotype 3 in acutely infected patient

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