Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers

Ruta angustifolia (L.) Pers., which is known as Garuda in Malaysia or Inggu in Indonesia is the only species in the genus Ruta of Rutaceae family that can be found in tropical climate. In Malaysia, it is usually grown as pot plant and use medicinally to treat skin diseases, boils and ear infection....

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Main Authors:	Mohd Hassan, Norazian, Abdol Latif, Fatimah, Tuan Aziz, Wan Hazni, Mohd Noor, Siti Sarah, Mohd Kamal, Laina Zarisa, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English English English English
Published:	2012
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/27124/ http://irep.iium.edu.my/27124/2/ISNPM_2012-ORAL_PRESENTATION-NORAZIAN.pdf http://irep.iium.edu.my/27124/7/ISNPM_2012-ABSTRACT_BOOK_COVER.pdf http://irep.iium.edu.my/27124/9/ISNPM_2012-ABSTRACTS.pdf http://irep.iium.edu.my/27124/10/OPT-037_ACCEPTANCE_LETTER.pdf http://irep.iium.edu.my/27124/11/22_NOV_2012.jpg

id	iium-27124
recordtype	eprints
spelling	iium-271242013-03-26T01:49:37Z http://irep.iium.edu.my/27124/ Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers Mohd Hassan, Norazian Abdol Latif, Fatimah Tuan Aziz, Wan Hazni Mohd Noor, Siti Sarah Mohd Kamal, Laina Zarisa Darnis, Deny Susanti QD Chemistry RS Pharmacy and materia medica Ruta angustifolia (L.) Pers., which is known as Garuda in Malaysia or Inggu in Indonesia is the only species in the genus Ruta of Rutaceae family that can be found in tropical climate. In Malaysia, it is usually grown as pot plant and use medicinally to treat skin diseases, boils and ear infection. In-vitro callus and shoot cultures have been established from its stem explants. Both tissues were multiplied, harvested and freeze dried for consecutive extraction with hexane, dichloromethane and methanol. The crude extracts from both tissues and intact plant were screened for their antimicrobial activity by disk diffusion assay against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 90028 and Candida parapsilosis ATCC 22019. The antimicrobial active compounds were isolated following bioassay-guided isolation approach. Agar overlay bioautography assay was performed to screen for the antimicrobial active compounds and the targeted active compounds were isolated by column chromatography. The antimicrobial activity of the isolated compound was evaluated quantitatively by using broth microdilution assay. The crude extracts of the in vitro cultures showed various degree of activity against the tested microbes and better activity as compared to that of intact plant. Three antimicrobial active alkaloids were isolated and identified as arborinine, graveoline and skimmianine. The alkaloids showed activity against all tested microbes with minimum inhibitory concentration (MIC) ranged between 62.5 µg/ml and 1000 µg/ml. 2012-11-22 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/27124/2/ISNPM_2012-ORAL_PRESENTATION-NORAZIAN.pdf application/pdf en http://irep.iium.edu.my/27124/7/ISNPM_2012-ABSTRACT_BOOK_COVER.pdf application/pdf en http://irep.iium.edu.my/27124/9/ISNPM_2012-ABSTRACTS.pdf application/pdf en http://irep.iium.edu.my/27124/10/OPT-037_ACCEPTANCE_LETTER.pdf application/pdf en http://irep.iium.edu.my/27124/11/22_NOV_2012.jpg Mohd Hassan, Norazian and Abdol Latif, Fatimah and Tuan Aziz, Wan Hazni and Mohd Noor, Siti Sarah and Mohd Kamal, Laina Zarisa and Darnis, Deny Susanti (2012) Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers. In: International Seminar on Natural Product Medicines 2012: Current Issues on Future Researchers and Applications of Natural Product Medicines, 22-23 Nov 2012, Bandung, Indonesia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Mohd Hassan, Norazian Abdol Latif, Fatimah Tuan Aziz, Wan Hazni Mohd Noor, Siti Sarah Mohd Kamal, Laina Zarisa Darnis, Deny Susanti Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers
description	Ruta angustifolia (L.) Pers., which is known as Garuda in Malaysia or Inggu in Indonesia is the only species in the genus Ruta of Rutaceae family that can be found in tropical climate. In Malaysia, it is usually grown as pot plant and use medicinally to treat skin diseases, boils and ear infection. In-vitro callus and shoot cultures have been established from its stem explants. Both tissues were multiplied, harvested and freeze dried for consecutive extraction with hexane, dichloromethane and methanol. The crude extracts from both tissues and intact plant were screened for their antimicrobial activity by disk diffusion assay against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 90028 and Candida parapsilosis ATCC 22019. The antimicrobial active compounds were isolated following bioassay-guided isolation approach. Agar overlay bioautography assay was performed to screen for the antimicrobial active compounds and the targeted active compounds were isolated by column chromatography. The antimicrobial activity of the isolated compound was evaluated quantitatively by using broth microdilution assay. The crude extracts of the in vitro cultures showed various degree of activity against the tested microbes and better activity as compared to that of intact plant. Three antimicrobial active alkaloids were isolated and identified as arborinine, graveoline and skimmianine. The alkaloids showed activity against all tested microbes with minimum inhibitory concentration (MIC) ranged between 62.5 µg/ml and 1000 µg/ml.
format	Conference or Workshop Item
author	Mohd Hassan, Norazian Abdol Latif, Fatimah Tuan Aziz, Wan Hazni Mohd Noor, Siti Sarah Mohd Kamal, Laina Zarisa Darnis, Deny Susanti
author_facet	Mohd Hassan, Norazian Abdol Latif, Fatimah Tuan Aziz, Wan Hazni Mohd Noor, Siti Sarah Mohd Kamal, Laina Zarisa Darnis, Deny Susanti
author_sort	Mohd Hassan, Norazian
title	Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers
title_short	Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers
title_full	Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers
title_fullStr	Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers
title_full_unstemmed	Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers
title_sort	antimicrobial activity of extracts and alkaloids from callus and shoot cultures of ruta angustifolia (l.) pers
publishDate	2012
url	http://irep.iium.edu.my/27124/ http://irep.iium.edu.my/27124/2/ISNPM_2012-ORAL_PRESENTATION-NORAZIAN.pdf http://irep.iium.edu.my/27124/7/ISNPM_2012-ABSTRACT_BOOK_COVER.pdf http://irep.iium.edu.my/27124/9/ISNPM_2012-ABSTRACTS.pdf http://irep.iium.edu.my/27124/10/OPT-037_ACCEPTANCE_LETTER.pdf http://irep.iium.edu.my/27124/11/22_NOV_2012.jpg
first_indexed	2023-09-18T20:40:20Z
last_indexed	2023-09-18T20:40:20Z
_version_	1777409322154721280

Antimicrobial activity of extracts and alkaloids from callus and shoot cultures of Ruta angustifolia (L.) Pers

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