Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion

Curry leaves VIZ. Murraya koenigii leaves (MKL) extract was found to possess robust antioxidant and anticholinesterase activities. Its memory enhancing effect in preclinical studies has also been reported. The current study was designed to evaluate the ultrastructural neurodegenerative changes withi...

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Main Authors:	Azzubaidi, Marwan Saad, Mohd Noor, Noriah, A.Talib, Norlelawati, Saxena, Anil Kumar, Al-Ani, Imad Matloub Dally, Maideen, Mohd Maizam
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/27084/ http://irep.iium.edu.my/27084/ http://irep.iium.edu.my/27084/1/Our_2012_EMSM_conference_Kota_Bharu.pdf

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recordtype	eprints
spelling	iium-270842015-12-18T03:02:59Z http://irep.iium.edu.my/27084/ Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion Azzubaidi, Marwan Saad Mohd Noor, Noriah A.Talib, Norlelawati Saxena, Anil Kumar Al-Ani, Imad Matloub Dally Maideen, Mohd Maizam RB Pathology Curry leaves VIZ. Murraya koenigii leaves (MKL) extract was found to possess robust antioxidant and anticholinesterase activities. Its memory enhancing effect in preclinical studies has also been reported. The current study was designed to evaluate the ultrastructural neurodegenerative changes within CA1 subfield of the hippocampus that result from chronic cerebral hypoperfusion (CCH) in rats, and to assess the possible preventive potential of MKL treatment to these changes. Rats were divided into sham-control, CCH and CCH+MKL treated groups. Ten weeks after surgical induction of CCH rats’ hippocampal sections were processed for transmission electron microscopical examination of CA1 subfield. Sham-control group exhibited normal pyramidal neurons with intact nuclei and abundant cytoplasmic organelles. CCH group hippocampi showed a mixture of necrotic and apoptotic neurodegeneration of pyramidal neurons with extensive loss of cytoplasmic organelles. Thickening of the endothelial basement membrane was observed with evident hypertrophy of astrocytic processes which constitute the vascular end-feet. CCH+MKL treated group demonstrated predominant apoptotic neurodegeneration. The pyramidal cells’ nuclei were shrunken with occasional formation of apoptotic bodies. Distorted intracellular organelles and abnormally thickened capillary endothelial basement membrane was noticed too. In conclusion, MKL treatment could not protect the CA1 hippocampal subfield from the CCH induced ultrastructural neurodegenerative changes. 2012-11-22 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/27084/1/Our_2012_EMSM_conference_Kota_Bharu.pdf Azzubaidi, Marwan Saad and Mohd Noor, Noriah and A.Talib, Norlelawati and Saxena, Anil Kumar and Al-Ani, Imad Matloub Dally and Maideen, Mohd Maizam (2012) Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion. In: 21st Scientific Conference of the Microscopy Society Malaysia, 22nd-24th November 2012, Renaissance Hotel, Kota Bharu, Kelantan, Malaysia. http://www.emsm2012.kk.usm.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Azzubaidi, Marwan Saad Mohd Noor, Noriah A.Talib, Norlelawati Saxena, Anil Kumar Al-Ani, Imad Matloub Dally Maideen, Mohd Maizam Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
description	Curry leaves VIZ. Murraya koenigii leaves (MKL) extract was found to possess robust antioxidant and anticholinesterase activities. Its memory enhancing effect in preclinical studies has also been reported. The current study was designed to evaluate the ultrastructural neurodegenerative changes within CA1 subfield of the hippocampus that result from chronic cerebral hypoperfusion (CCH) in rats, and to assess the possible preventive potential of MKL treatment to these changes. Rats were divided into sham-control, CCH and CCH+MKL treated groups. Ten weeks after surgical induction of CCH rats’ hippocampal sections were processed for transmission electron microscopical examination of CA1 subfield. Sham-control group exhibited normal pyramidal neurons with intact nuclei and abundant cytoplasmic organelles. CCH group hippocampi showed a mixture of necrotic and apoptotic neurodegeneration of pyramidal neurons with extensive loss of cytoplasmic organelles. Thickening of the endothelial basement membrane was observed with evident hypertrophy of astrocytic processes which constitute the vascular end-feet. CCH+MKL treated group demonstrated predominant apoptotic neurodegeneration. The pyramidal cells’ nuclei were shrunken with occasional formation of apoptotic bodies. Distorted intracellular organelles and abnormally thickened capillary endothelial basement membrane was noticed too. In conclusion, MKL treatment could not protect the CA1 hippocampal subfield from the CCH induced ultrastructural neurodegenerative changes.
format	Conference or Workshop Item
author	Azzubaidi, Marwan Saad Mohd Noor, Noriah A.Talib, Norlelawati Saxena, Anil Kumar Al-Ani, Imad Matloub Dally Maideen, Mohd Maizam
author_facet	Azzubaidi, Marwan Saad Mohd Noor, Noriah A.Talib, Norlelawati Saxena, Anil Kumar Al-Ani, Imad Matloub Dally Maideen, Mohd Maizam
author_sort	Azzubaidi, Marwan Saad
title	Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
title_short	Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
title_full	Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
title_fullStr	Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
title_full_unstemmed	Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
title_sort	ultrastructural assessment of the ca1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion
publishDate	2012
url	http://irep.iium.edu.my/27084/ http://irep.iium.edu.my/27084/ http://irep.iium.edu.my/27084/1/Our_2012_EMSM_conference_Kota_Bharu.pdf
first_indexed	2023-09-18T20:40:16Z
last_indexed	2023-09-18T20:40:16Z
_version_	1777409317906939904

Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion

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