Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa

Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa (ACT) have been shown to have antidiabetes activity. The present study was aimed to investigate the antidiabetic potential of the mixture of ACT in vivo and in vitro. A set dose of ACT (500, 125 and 75 mg/kg b.w. respectively) were a...

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Main Authors:	Bakhtiar, M. Taher, Mohamed Amiroudine, Mohamed Zaffar Ali, Tg Zakaria, Tg Muhammad Faris Syafiq, Wan Sulaiman, Wan Mohd. Azizi, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari
Format:	Article
Language:	English
Published:	Penerbit USM 2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/26473/ http://irep.iium.edu.my/26473/ http://irep.iium.edu.my/26473/1/Malaysian_Journal_of_Pharmacy.pdf

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recordtype	eprints
spelling	iium-264732014-12-09T00:25:18Z http://irep.iium.edu.my/26473/ Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tg Zakaria, Tg Muhammad Faris Syafiq Wan Sulaiman, Wan Mohd. Azizi Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari RS Pharmacy and materia medica Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa (ACT) have been shown to have antidiabetes activity. The present study was aimed to investigate the antidiabetic potential of the mixture of ACT in vivo and in vitro. A set dose of ACT (500, 125 and 75 mg/kg b.w. respectively) were administered to both normal and streptozotocin induced diabetic male Sprague-Dawley rats. The blood glucose levels were measured at 0, 3, 7, and 10 days after oral administration of ACT. Comparison was made with glibenclamide and placebo group. In in vitro method, the effect of ACT on lipid accumulation of 3T3-L1 adipocytes was analyzed by using Oil Red O staining. 2-Deoxy-D-[3H] glucose was used to measure glucose uptake activity. As the results, ACT exhibited blood lowering activity in streptozotocin induced diabetic rats. In cell based study, ACT was found to induce triglyceride accumulation in 3T3-L1 cells at relatively small concentration in a dose-dependent manner. In addition, 2-deoxy-D-[3H] glucose uptake activities were significantly different at dose 50 µg/mL (P < 0.05 compared to basal). Gene expression analysis using quantitative RT-PCR showed that adipocytes-treated with ACT significantly increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake when compared to the basal. Taken together, the results of this experimental study indicate that ACT possesses antihyperglycemic effects in vivo and in vitro. Penerbit USM 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/26473/1/Malaysian_Journal_of_Pharmacy.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Tg Zakaria, Tg Muhammad Faris Syafiq and Wan Sulaiman, Wan Mohd. Azizi and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari (2012) Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa. Malaysian Journal of Pharmaceutical Sciences, 1 (10). p. 116. ISSN 2180-429X http://www.mps.org.my/index.cfm?&menuid=146
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tg Zakaria, Tg Muhammad Faris Syafiq Wan Sulaiman, Wan Mohd. Azizi Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa
description	Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa (ACT) have been shown to have antidiabetes activity. The present study was aimed to investigate the antidiabetic potential of the mixture of ACT in vivo and in vitro. A set dose of ACT (500, 125 and 75 mg/kg b.w. respectively) were administered to both normal and streptozotocin induced diabetic male Sprague-Dawley rats. The blood glucose levels were measured at 0, 3, 7, and 10 days after oral administration of ACT. Comparison was made with glibenclamide and placebo group. In in vitro method, the effect of ACT on lipid accumulation of 3T3-L1 adipocytes was analyzed by using Oil Red O staining. 2-Deoxy-D-[3H] glucose was used to measure glucose uptake activity. As the results, ACT exhibited blood lowering activity in streptozotocin induced diabetic rats. In cell based study, ACT was found to induce triglyceride accumulation in 3T3-L1 cells at relatively small concentration in a dose-dependent manner. In addition, 2-deoxy-D-[3H] glucose uptake activities were significantly different at dose 50 µg/mL (P < 0.05 compared to basal). Gene expression analysis using quantitative RT-PCR showed that adipocytes-treated with ACT significantly increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake when compared to the basal. Taken together, the results of this experimental study indicate that ACT possesses antihyperglycemic effects in vivo and in vitro.
format	Article
author	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tg Zakaria, Tg Muhammad Faris Syafiq Wan Sulaiman, Wan Mohd. Azizi Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari
author_facet	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tg Zakaria, Tg Muhammad Faris Syafiq Wan Sulaiman, Wan Mohd. Azizi Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari
author_sort	Bakhtiar, M. Taher
title	Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa
title_short	Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa
title_full	Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa
title_fullStr	Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa
title_full_unstemmed	Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa
title_sort	study on antidiabetic plants cocktail containing andrographis paniculata, cinnamomum burmannii and tinospora crispa
publisher	Penerbit USM
publishDate	2012
url	http://irep.iium.edu.my/26473/ http://irep.iium.edu.my/26473/ http://irep.iium.edu.my/26473/1/Malaysian_Journal_of_Pharmacy.pdf
first_indexed	2023-09-18T20:39:26Z
last_indexed	2023-09-18T20:39:26Z
_version_	1777409266118819840

Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa

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