The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage

This study was designed to verify the optimal· basic culture media that promote chondrocytes proliferation in vitro in order to facilitate adequate amount of chondrocytes for cartilage reconstructionas well as maintaining cartilage specific phenotype. Human articular chondrocytes were cultured in th...

Full description

Bibliographic Details
Main Authors:	Sha'ban, Munirah, Saim, Aminuddin, Casim, Samsudin Osman, Kienhui, Chua, Nor Hussien, Fuzina, Idrus, Ruszymah
Format:	Article
Language:	English
Published:	John Wiley & Sons, Ltd. 2005
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/26448/ http://irep.iium.edu.my/26448/1/KTERMS2005_Munirah_et_al.pdf

id	iium-26448
recordtype	eprints
spelling	iium-264482013-07-12T01:50:16Z http://irep.iium.edu.my/26448/ The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage Sha'ban, Munirah Saim, Aminuddin Casim, Samsudin Osman Kienhui, Chua Nor Hussien, Fuzina Idrus, Ruszymah R Medicine (General) This study was designed to verify the optimal· basic culture media that promote chondrocytes proliferation in vitro in order to facilitate adequate amount of chondrocytes for cartilage reconstructionas well as maintaining cartilage specific phenotype. Human articular chondrocytes were cultured in three types of basic culture media Ham's F12, DMEM and the equivalent mixture of F12:DMEM. Cultured chondrocytes were trypsinized as they reached confluency. The viability and total number of cell were recorded at every passage. Large-scale culture expansion was used to reconstruct tissue-engineered cartilage. Quantitative RT-PCR analysis was used to evaluate the expression of collagen Type II, collagen Type I, aggrecan and Sox 9 gene, both in monolayer culture and in the engineered cartilage. The mixture of F12:DMEM promotes significantly greater (p<0.05) chondrocytes proliferation at every passage compared to the individual medium. Monolayer cultured chondrocytes exhibited down-regulation expression pattern of collagen Type II gene, aggrecan and Sox 9, whilst the expression of collagen Type I is up-regulated. Tissue-engineered cartilage morphologically and histologically resembled normal hyaline cartilage. Moreover, tissue-engineered cartilage re-expressed the specific chondrogenesis markers; collagen Type II, aggrecan and Sox 9. In conclusion, the mixture of F12:DMEM enhanced human articular chondrocytes proliferation thus provided adequate amount of chondrocytes for cartilage reconstruction. The new cartilage formed phenotypically resembles native cartilage. This results hold promise for the use of tissue-engineered cartilage implant for future orthopaedic reconstructive surgery John Wiley & Sons, Ltd. 2005 Article PeerReviewed application/pdf en http://irep.iium.edu.my/26448/1/KTERMS2005_Munirah_et_al.pdf Sha'ban, Munirah and Saim, Aminuddin and Casim, Samsudin Osman and Kienhui, Chua and Nor Hussien, Fuzina and Idrus, Ruszymah (2005) The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage. Tissue Engineering and Regenerative Medicine, 2 (4). pp. 347-355. ISSN 1932-7005
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Sha'ban, Munirah Saim, Aminuddin Casim, Samsudin Osman Kienhui, Chua Nor Hussien, Fuzina Idrus, Ruszymah The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
description	This study was designed to verify the optimal· basic culture media that promote chondrocytes proliferation in vitro in order to facilitate adequate amount of chondrocytes for cartilage reconstructionas well as maintaining cartilage specific phenotype. Human articular chondrocytes were cultured in three types of basic culture media Ham's F12, DMEM and the equivalent mixture of F12:DMEM. Cultured chondrocytes were trypsinized as they reached confluency. The viability and total number of cell were recorded at every passage. Large-scale culture expansion was used to reconstruct tissue-engineered cartilage. Quantitative RT-PCR analysis was used to evaluate the expression of collagen Type II, collagen Type I, aggrecan and Sox 9 gene, both in monolayer culture and in the engineered cartilage. The mixture of F12:DMEM promotes significantly greater (p<0.05) chondrocytes proliferation at every passage compared to the individual medium. Monolayer cultured chondrocytes exhibited down-regulation expression pattern of collagen Type II gene, aggrecan and Sox 9, whilst the expression of collagen Type I is up-regulated. Tissue-engineered cartilage morphologically and histologically resembled normal hyaline cartilage. Moreover, tissue-engineered cartilage re-expressed the specific chondrogenesis markers; collagen Type II, aggrecan and Sox 9. In conclusion, the mixture of F12:DMEM enhanced human articular chondrocytes proliferation thus provided adequate amount of chondrocytes for cartilage reconstruction. The new cartilage formed phenotypically resembles native cartilage. This results hold promise for the use of tissue-engineered cartilage implant for future orthopaedic reconstructive surgery
format	Article
author	Sha'ban, Munirah Saim, Aminuddin Casim, Samsudin Osman Kienhui, Chua Nor Hussien, Fuzina Idrus, Ruszymah
author_facet	Sha'ban, Munirah Saim, Aminuddin Casim, Samsudin Osman Kienhui, Chua Nor Hussien, Fuzina Idrus, Ruszymah
author_sort	Sha'ban, Munirah
title	The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
title_short	The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
title_full	The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
title_fullStr	The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
title_full_unstemmed	The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
title_sort	re-expression of collagen type ii, aggrecan and sox 9 in tissue-engineered human articular cartilage
publisher	John Wiley & Sons, Ltd.
publishDate	2005
url	http://irep.iium.edu.my/26448/ http://irep.iium.edu.my/26448/1/KTERMS2005_Munirah_et_al.pdf
first_indexed	2023-09-18T20:39:25Z
last_indexed	2023-09-18T20:39:25Z
_version_	1777409264242917376

The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage

Similar Items