Characterization of drug-release kinetics in trabecular bone from titania nanotube implants

Purpose: The aim of this study was to investigate the application of the three-dimensional bone bioreactor for studying drug-release kinetics and distribution of drugs in the ex vivo cancellous bone environment, and to demonstrate the application of nanoengineered titanium (Ti) wires generated with...

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Main Authors:	Moom, Sin Aw, Khalid, Kamarul Ariffin, Gulati, Karan, J Atkins, Gerald, Pivonka, Peter, Findlay, David M., Losic, Dusan
Format:	Article
Language:	English
Published:	DOVE Medical Press, Auckland 2012
Subjects:	R Medicine (General) RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/26255/ http://irep.iium.edu.my/26255/ http://irep.iium.edu.my/26255/1/f_IJN-33655-characterization-of-drug-release-kinetics-in-trabecular-bone_091112_13927.pdf

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spelling	iium-262552012-11-06T05:36:28Z http://irep.iium.edu.my/26255/ Characterization of drug-release kinetics in trabecular bone from titania nanotube implants Moom, Sin Aw Khalid, Kamarul Ariffin Gulati, Karan J Atkins, Gerald Pivonka, Peter Findlay, David M. Losic, Dusan R Medicine (General) RM300 Drugs and their action Purpose: The aim of this study was to investigate the application of the three-dimensional bone bioreactor for studying drug-release kinetics and distribution of drugs in the ex vivo cancellous bone environment, and to demonstrate the application of nanoengineered titanium (Ti) wires generated with titania nanotube (TNT) arrays as drug-releasing implants for local drug delivery Methods: Nanoengineered Ti wires covered with a layer of TNT arrays implanted in bone were used as a drug-releasing implant. Viable bovine trabecular bone was used as the ex vivo bone substrate embedded with the implants and placed in the bone reactor. A hydrophilic fluorescent dye (rhodamine B) was used as the model drug, loaded inside the TNT–Ti implants, to monitor drug release and transport in trabecular bone. The distribution of released model drug in the bone was monitored throughout the bone structure, and concentration profiles at different vertical (0–5 mm) and horizontal (0–10 mm) distances from the implant surface were obtained at a range of release times from 1 hour to 5 days. Results: Scanning electron microscopy confirmed that well-ordered, vertically aligned nanotube arrays were formed on the surface of prepared TNT–Ti wires. Thermogravimetric analysis proved loading of the model drug and fluorescence spectroscopy was used to show drug-release characteristics in-vitro. The drug release from implants inserted into bone ex vivo showed a consistent gradual release of model drug from the TNT–Ti implants, with a characteristic three-dimensional distribution into the surrounding bone, over a period of 5 days. The parameters including the flow rate of bone culture medium, differences in trabecular microarchitecture between bone samples, and mechanical loading were found to have the most significant influence on drug distribution in the bone. Conclusion: These results demonstrate the utility of the Zetos™ system for ex vivo drug-release studies in bone, which can be applied to optimize the delivery of specific therapies and to assist in the design of new drug delivery systems. This method has the potential to provide new knowledge to understand drug distribution in the bone environment and to considerably improve existing technologies for local administration in bone, including solving some critical problems in bone therapy and orthopedic implants. DOVE Medical Press, Auckland 2012-09-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/26255/1/f_IJN-33655-characterization-of-drug-release-kinetics-in-trabecular-bone_091112_13927.pdf Moom, Sin Aw and Khalid, Kamarul Ariffin and Gulati, Karan and J Atkins, Gerald and Pivonka, Peter and Findlay, David M. and Losic, Dusan (2012) Characterization of drug-release kinetics in trabecular bone from titania nanotube implants. International Journal of Nanomedicine (7). pp. 4883-4892. ISSN 1178-2013 http://www.dovepress.com/characterization-of-drug-release-kinetics-in-trabecular-bone-from-tita-peer-reviewed-article-IJN
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RM300 Drugs and their action
spellingShingle	R Medicine (General) RM300 Drugs and their action Moom, Sin Aw Khalid, Kamarul Ariffin Gulati, Karan J Atkins, Gerald Pivonka, Peter Findlay, David M. Losic, Dusan Characterization of drug-release kinetics in trabecular bone from titania nanotube implants
description	Purpose: The aim of this study was to investigate the application of the three-dimensional bone bioreactor for studying drug-release kinetics and distribution of drugs in the ex vivo cancellous bone environment, and to demonstrate the application of nanoengineered titanium (Ti) wires generated with titania nanotube (TNT) arrays as drug-releasing implants for local drug delivery Methods: Nanoengineered Ti wires covered with a layer of TNT arrays implanted in bone were used as a drug-releasing implant. Viable bovine trabecular bone was used as the ex vivo bone substrate embedded with the implants and placed in the bone reactor. A hydrophilic fluorescent dye (rhodamine B) was used as the model drug, loaded inside the TNT–Ti implants, to monitor drug release and transport in trabecular bone. The distribution of released model drug in the bone was monitored throughout the bone structure, and concentration profiles at different vertical (0–5 mm) and horizontal (0–10 mm) distances from the implant surface were obtained at a range of release times from 1 hour to 5 days. Results: Scanning electron microscopy confirmed that well-ordered, vertically aligned nanotube arrays were formed on the surface of prepared TNT–Ti wires. Thermogravimetric analysis proved loading of the model drug and fluorescence spectroscopy was used to show drug-release characteristics in-vitro. The drug release from implants inserted into bone ex vivo showed a consistent gradual release of model drug from the TNT–Ti implants, with a characteristic three-dimensional distribution into the surrounding bone, over a period of 5 days. The parameters including the flow rate of bone culture medium, differences in trabecular microarchitecture between bone samples, and mechanical loading were found to have the most significant influence on drug distribution in the bone. Conclusion: These results demonstrate the utility of the Zetos™ system for ex vivo drug-release studies in bone, which can be applied to optimize the delivery of specific therapies and to assist in the design of new drug delivery systems. This method has the potential to provide new knowledge to understand drug distribution in the bone environment and to considerably improve existing technologies for local administration in bone, including solving some critical problems in bone therapy and orthopedic implants.
format	Article
author	Moom, Sin Aw Khalid, Kamarul Ariffin Gulati, Karan J Atkins, Gerald Pivonka, Peter Findlay, David M. Losic, Dusan
author_facet	Moom, Sin Aw Khalid, Kamarul Ariffin Gulati, Karan J Atkins, Gerald Pivonka, Peter Findlay, David M. Losic, Dusan
author_sort	Moom, Sin Aw
title	Characterization of drug-release kinetics in trabecular bone from titania nanotube implants
title_short	Characterization of drug-release kinetics in trabecular bone from titania nanotube implants
title_full	Characterization of drug-release kinetics in trabecular bone from titania nanotube implants
title_fullStr	Characterization of drug-release kinetics in trabecular bone from titania nanotube implants
title_full_unstemmed	Characterization of drug-release kinetics in trabecular bone from titania nanotube implants
title_sort	characterization of drug-release kinetics in trabecular bone from titania nanotube implants
publisher	DOVE Medical Press, Auckland
publishDate	2012
url	http://irep.iium.edu.my/26255/ http://irep.iium.edu.my/26255/ http://irep.iium.edu.my/26255/1/f_IJN-33655-characterization-of-drug-release-kinetics-in-trabecular-bone_091112_13927.pdf
first_indexed	2023-09-18T20:39:08Z
last_indexed	2023-09-18T20:39:08Z
_version_	1777409246600626176

Characterization of drug-release kinetics in trabecular bone from titania nanotube implants

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