Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION

Recent advancements in recombinant technology have proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of protein in a nonnative host microorganism, the overall production...

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Main Authors:	Othman, Muhd. Ezza Faiez, Amid, Azura, Jimat, Dzun Noraini, Jamaluddin, Mohd Jamil Aizat
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	T175 Industrial research. Research and development
Online Access:	http://irep.iium.edu.my/26150/ http://irep.iium.edu.my/26150/1/%5BBPE-106%5D__Muhd_Ezza_Faiez_Othman_Microtribe_2012.pdf

id	iium-26150
recordtype	eprints
spelling	iium-261502013-01-04T03:00:21Z http://irep.iium.edu.my/26150/ Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat T175 Industrial research. Research and development Recent advancements in recombinant technology have proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of protein in a nonnative host microorganism, the overall production processes are not quite that straightforward as several common challenges, such as protein degradation, especially during cell disruption stage. As the process has been subjected to both protein and hostspecific, a systematic process “conditioning” for maximal production of recombinant protein is therefore required. In this study, a simple approach to determine optimal conditions for cell disruption using high-pressure homogenizer method to isolate recombinant bromelian from E. coli BL21-AI is reported. One-Factor-At-A-Time (OFAT) approach was initially used to determine optimal test range for each parameter, namely, pressure (10-50 MPa), flow rate (10-20 psi) and number of passes (1-3 cycles). Next, by response surface methodology (RSM) a three factors face-centered central composite design (FCCD) was applied to obtain optimal process conditions Then the prediction model was validated. Under the optimal conditions, the specific enzyme activity of the recombinant bromelain was found to be functionally and reproducibly acceptable at 0.037 U/mg. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/26150/1/%5BBPE-106%5D__Muhd_Ezza_Faiez_Othman_Microtribe_2012.pdf Othman, Muhd. Ezza Faiez and Amid, Azura and Jimat, Dzun Noraini and Jamaluddin, Mohd Jamil Aizat (2012) Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION. In: Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE 2009), 12-13 December 2012, Langkawi, Kedah, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T175 Industrial research. Research and development
spellingShingle	T175 Industrial research. Research and development Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
description	Recent advancements in recombinant technology have proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of protein in a nonnative host microorganism, the overall production processes are not quite that straightforward as several common challenges, such as protein degradation, especially during cell disruption stage. As the process has been subjected to both protein and hostspecific, a systematic process “conditioning” for maximal production of recombinant protein is therefore required. In this study, a simple approach to determine optimal conditions for cell disruption using high-pressure homogenizer method to isolate recombinant bromelian from E. coli BL21-AI is reported. One-Factor-At-A-Time (OFAT) approach was initially used to determine optimal test range for each parameter, namely, pressure (10-50 MPa), flow rate (10-20 psi) and number of passes (1-3 cycles). Next, by response surface methodology (RSM) a three factors face-centered central composite design (FCCD) was applied to obtain optimal process conditions Then the prediction model was validated. Under the optimal conditions, the specific enzyme activity of the recombinant bromelain was found to be functionally and reproducibly acceptable at 0.037 U/mg.
format	Conference or Workshop Item
author	Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat
author_facet	Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat
author_sort	Othman, Muhd. Ezza Faiez
title	Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
title_short	Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
title_full	Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
title_fullStr	Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
title_full_unstemmed	Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
title_sort	optimization of cell discruption for recombinant bromelain using high pressure homogenizer (hph) for commercial production commercial production
publishDate	2012
url	http://irep.iium.edu.my/26150/ http://irep.iium.edu.my/26150/1/%5BBPE-106%5D__Muhd_Ezza_Faiez_Othman_Microtribe_2012.pdf
first_indexed	2023-09-18T20:38:59Z
last_indexed	2023-09-18T20:38:59Z
_version_	1777409237704507392

Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION

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