Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain

In this study, bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli strain BL21-AI using auto-inductionbased fermentation media. Recently, many recombinant proteins efficiently expressed in soluble form in E. coli using this auto-indu...

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Main Authors:	Jamaluddin, Mohd Jamil Aizat, Amid, Azura, Azmi, Azlin Suhaida, Othman, Muhd. Ezza Faiez
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	T175 Industrial research. Research and development
Online Access:	http://irep.iium.edu.my/26148/ http://irep.iium.edu.my/26148/1/BPE-103_Mohd_Jamil_Aizat_Mocotribe_2012.pdf

id	iium-26148
recordtype	eprints
spelling	iium-261482013-01-04T03:02:05Z http://irep.iium.edu.my/26148/ Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez T175 Industrial research. Research and development In this study, bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli strain BL21-AI using auto-inductionbased fermentation media. Recently, many recombinant proteins efficiently expressed in soluble form in E. coli using this auto-induction approach. Indeed, our preliminary experiment showed that this media was proven to be able in producing a high-density batch culture as claimed. Still, since overall soluble expression level is thought to be both protein and host’s strain specific depending on the type of media used, further optimization step for legitimate optimal production of recombinant bromelain is therefore required. Moreover, to our knowledge, as of now optimization study on performance of the production of recombinant bromelain in E. coli using the autoinduction media has yet been initiated elsewhere in the literature. Statistical analysis of results shows that complex nitrogen sources, carbon sources, buffering salts were indeed significant in increasing the biomass production. The optimized values of these three factors were obtained by RSM based on the result of a face-centered central composite design. Under these proposed optimized medium, the model predicted a biomass productivity of 5.567 g/l and its corresponding recombinant bromelain specific activity was reasonably well at 0.1976 U/mg. 2009 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/26148/1/BPE-103_Mohd_Jamil_Aizat_Mocotribe_2012.pdf Jamaluddin, Mohd Jamil Aizat and Amid, Azura and Azmi, Azlin Suhaida and Othman, Muhd. Ezza Faiez (2009) Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain. In: Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE 2009), 12-13 December 2009, PWTC, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T175 Industrial research. Research and development
spellingShingle	T175 Industrial research. Research and development Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
description	In this study, bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli strain BL21-AI using auto-inductionbased fermentation media. Recently, many recombinant proteins efficiently expressed in soluble form in E. coli using this auto-induction approach. Indeed, our preliminary experiment showed that this media was proven to be able in producing a high-density batch culture as claimed. Still, since overall soluble expression level is thought to be both protein and host’s strain specific depending on the type of media used, further optimization step for legitimate optimal production of recombinant bromelain is therefore required. Moreover, to our knowledge, as of now optimization study on performance of the production of recombinant bromelain in E. coli using the autoinduction media has yet been initiated elsewhere in the literature. Statistical analysis of results shows that complex nitrogen sources, carbon sources, buffering salts were indeed significant in increasing the biomass production. The optimized values of these three factors were obtained by RSM based on the result of a face-centered central composite design. Under these proposed optimized medium, the model predicted a biomass productivity of 5.567 g/l and its corresponding recombinant bromelain specific activity was reasonably well at 0.1976 U/mg.
format	Conference or Workshop Item
author	Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez
author_facet	Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez
author_sort	Jamaluddin, Mohd Jamil Aizat
title	Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
title_short	Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
title_full	Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
title_fullStr	Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
title_full_unstemmed	Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
title_sort	optimization of auto-introduction based fermentation media components for biomass production of escherichia coli bl21-ai expressing recombinant bromelain
publishDate	2009
url	http://irep.iium.edu.my/26148/ http://irep.iium.edu.my/26148/1/BPE-103_Mohd_Jamil_Aizat_Mocotribe_2012.pdf
first_indexed	2023-09-18T20:38:59Z
last_indexed	2023-09-18T20:38:59Z
_version_	1777409237419294720

Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain

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