Optimization of auto-introduction based fermentation media components for biomass production of Escherichia coli BL21-AI expressing recombinant bromelain
In this study, bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli strain BL21-AI using auto-inductionbased fermentation media. Recently, many recombinant proteins efficiently expressed in soluble form in E. coli using this auto-indu...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/26148/ http://irep.iium.edu.my/26148/1/BPE-103_Mohd_Jamil_Aizat_Mocotribe_2012.pdf |
| Summary: | In this study, bromelain, a naturally available therapeutic protease enzyme from
pineapple stem, was expressed in Escherichia coli strain BL21-AI using auto-inductionbased
fermentation media. Recently, many recombinant proteins efficiently expressed in
soluble form in E. coli using this auto-induction approach. Indeed, our preliminary
experiment showed that this media was proven to be able in producing a high-density
batch culture as claimed. Still, since overall soluble expression level is thought to be
both protein and host’s strain specific depending on the type of media used, further
optimization step for legitimate optimal production of recombinant bromelain is
therefore required. Moreover, to our knowledge, as of now optimization study on
performance of the production of recombinant bromelain in E. coli using the autoinduction
media has yet been initiated elsewhere in the literature. Statistical analysis of
results shows that complex nitrogen sources, carbon sources, buffering salts were
indeed significant in increasing the biomass production. The optimized values of these
three factors were obtained by RSM based on the result of a face-centered central
composite design. Under these proposed optimized medium, the model predicted a
biomass productivity of 5.567 g/l and its corresponding recombinant bromelain specific
activity was reasonably well at 0.1976 U/mg. |
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