BT-201: Investigation of the spider web for antibacterial activity

Spiders build their webs with a material called silk. Spider silk contain protein fiber that have many advantages and functions. One of them is to capture their prey such as flies, insects, and others. The needs on the research of antibacterial activity are important for human health because of impo...

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Main Authors:	Mirghani, Mohamed Elwathig Saeed, Kabbashi, Nassereldeen Ahmed, Elfaki, Faiz Ahmed Mohamed, Zulkifli, M. Z. Fahmi
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/26101/ http://irep.iium.edu.my/26101/ http://irep.iium.edu.my/26101/1/%5BBT-201%5D_Mohamed_Elwathiq.pdf

id	iium-26101
recordtype	eprints
spelling	iium-261012012-12-06T06:29:08Z http://irep.iium.edu.my/26101/ BT-201: Investigation of the spider web for antibacterial activity Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Elfaki, Faiz Ahmed Mohamed Zulkifli, M. Z. Fahmi QR Microbiology Spiders build their webs with a material called silk. Spider silk contain protein fiber that have many advantages and functions. One of them is to capture their prey such as flies, insects, and others. The needs on the research of antibacterial activity are important for human health because of importance of finding a new cure for some diseases that occur because of microorganisms. Some of the microorganisms, especially bacteria are becoming resistant to many antibacterial agents. The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted using different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, and concentration used for optimization). These extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria were used in the antibacterial assay namely Bacillus subtilis, and Escherichia coli. The determination of spider webs exhibiting antibacterial properties was based, at least in this study, solely upon the definite appearance of inhibition zone around the well of plates. In screening, acetone solvent was shown the best for antibacterial activity compare to other solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition zone on the Bacillus subtilis was 15 mm at a time of 48 hours and concentration of 0.035 g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was 12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study showed that spider webs could be potential source of new antibacterial agents. 2012-07-04 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/26101/1/%5BBT-201%5D_Mohamed_Elwathiq.pdf Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed and Elfaki, Faiz Ahmed Mohamed and Zulkifli, M. Z. Fahmi (2012) BT-201: Investigation of the spider web for antibacterial activity. In: 2nd Malaysian International Conference on Trends In Bioprocess Engineering (MICOTriBE 2012), 3–5 July 2012., Meritus Pelangi Beach Resort & Spa Langkawi, Kedah, Malaysia. http://micotribe2012.unimap.edu.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Elfaki, Faiz Ahmed Mohamed Zulkifli, M. Z. Fahmi BT-201: Investigation of the spider web for antibacterial activity
description	Spiders build their webs with a material called silk. Spider silk contain protein fiber that have many advantages and functions. One of them is to capture their prey such as flies, insects, and others. The needs on the research of antibacterial activity are important for human health because of importance of finding a new cure for some diseases that occur because of microorganisms. Some of the microorganisms, especially bacteria are becoming resistant to many antibacterial agents. The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted using different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, and concentration used for optimization). These extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria were used in the antibacterial assay namely Bacillus subtilis, and Escherichia coli. The determination of spider webs exhibiting antibacterial properties was based, at least in this study, solely upon the definite appearance of inhibition zone around the well of plates. In screening, acetone solvent was shown the best for antibacterial activity compare to other solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition zone on the Bacillus subtilis was 15 mm at a time of 48 hours and concentration of 0.035 g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was 12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study showed that spider webs could be potential source of new antibacterial agents.
format	Conference or Workshop Item
author	Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Elfaki, Faiz Ahmed Mohamed Zulkifli, M. Z. Fahmi
author_facet	Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Elfaki, Faiz Ahmed Mohamed Zulkifli, M. Z. Fahmi
author_sort	Mirghani, Mohamed Elwathig Saeed
title	BT-201: Investigation of the spider web for antibacterial activity
title_short	BT-201: Investigation of the spider web for antibacterial activity
title_full	BT-201: Investigation of the spider web for antibacterial activity
title_fullStr	BT-201: Investigation of the spider web for antibacterial activity
title_full_unstemmed	BT-201: Investigation of the spider web for antibacterial activity
title_sort	bt-201: investigation of the spider web for antibacterial activity
publishDate	2012
url	http://irep.iium.edu.my/26101/ http://irep.iium.edu.my/26101/ http://irep.iium.edu.my/26101/1/%5BBT-201%5D_Mohamed_Elwathiq.pdf
first_indexed	2023-09-18T20:38:54Z
last_indexed	2023-09-18T20:38:54Z
_version_	1777409232524541952

BT-201: Investigation of the spider web for antibacterial activity

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